Empowerment Program in Pesagi Village Community: Assistance in Compounding Innovative Peel-off Pain Reliever (UNO) Derived from Family Medicinal Plants

The abundance of family medicinal plants (TOGA) growing in the yards of the house can be utilized for one of the health products called boreh, a traditional medicine used from generation to generation by the Balinese. Boreh can be innovated into a peel-off preparation form that has some potential to increase public interest in traditional medicine. The contents of TOGA, such as ginger, turmeric, and galangal in the peel-off boreh preparation, may exhibit beneficial effects as a pain reliever. Therefore, the mentioned product can be used to overcome pain problems that are commonly found in the people of Pesagi Village. The ideas related to compounding certain innovative herbal preparations for overcoming health problems in Pesagi Village were carried out through community empowerment activities. A series of program activities took place during January-April 2022. The purpose of this community empowerment program was to provide training and assistance activities to the people of Pesagi Village in the practice of utilizing TOGA as an innovative boreh peel-off preparation, namely UNO, with empirical pain reliever properties. Guidance and assistance in compounding practice were based on the results of dosage formulation previously developed by the executive team in the School of Pharmacy Mahaganesha Laboratory. This empowerment program was executed through a learning-by-doing approach, integrated training, participatory practice, and guided mentoring by the executive team with knowledge and skills evaluation (pre-test and post-test, p=0.000, 95%). After the empowerment program, the community has been able to practice how to turn TOGA from the ground into a useful product. The innovative products in this empowerment program may offer some potential benefits to overcome certain health-related problems of the community in Pesagi Village, such as joint pain. The success of this program may encourage the sustainability of the program in the future

Total Flavonoid Levels in n-hexane and Ethyl Acetate Fractions of Rosmarinus officinalis L. Leaves and Their Antibacterial and Antioxidant Activities

The rosemary (Rosmarinus officinalis L.) is a plant of the Lamiaceae tribe that has not been widely studied regarding its pharmacological activity, known from previous studies to contain secondary metabolites of flavonoids. Flavonoids are phenol compounds with many pharmacological activities, including antibacterials and antioxidants. This study aims to determine the total flavonoid levels in R. officinalis leaves and their effect on antibacterial and antioxidant activities. This research began with the preparation of ethanol extract from R. officinalis leaves, then the fractionation of the extract produced n-hexane and ethyl acetate fractions. Total flavonoid levels were determined against both fractions by UV-Vis spectrophotometry. A test of the fraction’s antibacterial activity against Staphylococcus aureus was performed using the disc diffusion method. The antioxidant test is carried out by the DPPH method. The total flavonoid content of the ethyl acetate fraction is 47.437 ± 1.947%, higher than the n-hexane fraction. Test antibacterial and antioxidant activity showed more significant results in the ethyl acetate fraction than in the n-hexane fraction. In conclusion, the total flavonoid levels of ethyl acetate fraction are directly proportional to the antibacterial and antioxidant activities of R. officinalis leaves