Ultrastructural study on morphology of Schistosoma spindale by Scanning electron microscopy (SEM)

The present study was undertaken to investigate the detailed morphological features along with morphometry of different structures of Schistosoma spindale (Adult flukes) which were recovered by a perfusion technique and visualized by Scanning Electron Microscopy (SEM). The length of spines on the oral sucker and ventral suckers were 2.6 µm and 2.5 µm, respectively. The measured width of aspinose area beneath the ventral sucker, rim of the ventral sucker and tegumental papillae were 5.4, 22.5 µm and 3 µm, respectively. Males have a welldefined gynaecophoric canal, originating just below the ventral sucker and extending up to the posterior end of the body, continued as a marked conical projection. The ventral surface of the oral sucker was completely covered with numerous spines. The ventral sucker was pedunculated, round, thick-rimmed and the inner side contained numerously pointed spines directed towards the center of the ventral sucker. The tegument surface of S. spindale showed ridged layers with large uniciliated and pit like papillae which were recorded more in posterior end. Thus, Scanning Electron microscopy (SEM) provided indepth ultrastructural morphological details of Schistosoma spindale which was in accordance with that of previous studies, would be applicable for its differentiation with other species (S. mansoni, S. bovis, S. haematobium, S. japonicum)