リュツォ・ホルム湾，プリンスオラフ海岸，及び，エンダビーランド地質調査隊報告2016-2017（JARE-58）

第58次日本南極地域観測隊（JARE-58）では，2016−2017の夏期期間にリュツォ・ホルム湾，プリンスオラフ海岸，及び，エンダビーランドにおいて地質調査をおこなった．調査隊のメンバーは，日本人地質研究者4名とアジア地域（タイ，インドネシア，モンゴル）の交換科学者3名で構成され，本吉隊長が一部期間の調査に加わった．第58次夏期観測では，「しらせ」搭載の2機の大型ヘリコプター（CH101）とともに観測隊チャーターの小型ヘリコプター（AS350）1機による野外調査の支援がなされた．本稿では，観測計画を実施するための，主に設営面での計画，準備，そして行動経過について報告する．The 58th Japanese Antarctic Research Expedition (JARE-58) conducted geological field surveys in the regions of Lützow-Holm Bay, Prince Olav Coast, and Enderby Land during the 2016−2017 austral summer season. The field party consisted of four Japanese geologists and three Asian geologists (Thai, Indonesian, Mongolian), and was joined periodically by JARE-58 expedition leader, Prof. Motoyoshi. Field parties were supported throughout the summer season by a smaller secondary helicopter (AS350) in addition to two main helicopters (CH101) stationed on the icebreaker Shirase. This report summarizes field preparations and the geological work undertaken, and highlights several key points for future planning and research

Establishment of transient gene expression systems in protoplasts from <i>Liriodendron</i> hybrid mesophyll cells

<div><p><i>Liriodendron</i> is a genus of the magnolia family comprised of two flowering tree species that produce hardwoods of great ecological and economic value. However, only a limited amount of genetic research has been performed on the <i>Liriodendron</i> genus partly because transient or stable transgenic trees have been difficult to produce. In general, transient expression systems are indispensable for rapid, high-throughput screening and systematic characterization of gene functions at a low cost; therefore, development of such a system for <i>Liriodendron</i> would provide a necessary step forward for research on Magnoliaceae and other woody trees. Herein, we describe an efficient and rapid protocol for preparing protoplasts from the leaf mesophyll tissue of a <i>Liriodendron</i> hybrid and an optimized system for polyethylene glycol–mediated transient transfection of the protoplasts. Because the leaves of the <i>Liriodendron</i> hybrid are waxy, we formulated an enzyme mix containing 1.5% (w/v) Cellulase R-10, 0.5% (w/v) Macerozyme R-10, and 0.1% (w/v) Pectolyase Y-23 to efficiently isolate protoplasts from the <i>Liriodendron</i> hybrid leaf mesophyll tissue in 3 h. We optimized <i>Liriodendron</i> protoplast transfection efficiency by including 20 μg plasmid DNA per 10⁴ protoplasts, a transformation time of 20 min, and inclusion of 20% (w/v) polyethylene glycol 4000. After integrating the <i>Liriodendron WOX1</i> gene into pJIT166-GFP to produce a <i>WOX1</i>-GFP fusion product and transfecting it into isolated protoplasts, Lh<i>WOX1</i>-GFP was found to localize to the nucleus according to its green fluorescence.</p></div