Comparison of Phenolics and Phenolic Acid Profiles in Conjunction with Oxygen Radical Absorbing Capacity (ORAC) in Berries of Vaccinium arctostaphylos L. and V. myrtillus L.

Strnad, Miroslav/0000-0002-2806-794X; GRUZ, Jiri/0000-0002-8546-9697; Gruz, Jiri/0000-0002-8546-9697WOS: 000375613200003Caucasian blueberry (Vaccinium arctostaphylos L.) and bilberry (V. myrtillus L.), both native to Turkey, were evaluated for their total phenolics (TP) and anthocyanin (TAC) contents. Individual compositions of free phenolic acids and phenolic acids liberated from ester and glycoside forms were analyzed using UPLC-MS/MS. Berry extracts of each species were separated into three different fractions (sugar/acid, polyphenolic and anthocyanin) by solid phase extraction (SPE). The anthocyanin fractions of each species had the highest level of TP and TAC contents and ORAC values. Each species contained 10 phenolic acids representing each fraction, but in different quantities. The phenolic acids liberated from the esters and glycoside forms were caffeic acid and p-coumaric acid. The fractions containing phenolic acids liberated from ester and glycoside forms had a higher antioxidant capacity than that from free phenolic acids. The data suggest that both berries have potential as good dietary sources of phenolic antioxidants.Research Fund of Karadeniz Technical University [2005.111.004.02]; Council of Higher Education of Turkey (CoHE)Ministry of National Education - Turkey; Ministry of Education, Youth and Sports of the Czech Republic through the National Program of Sustainability [LO1204]Financial support for this study was provided by the Research Fund of Karadeniz Technical University (Project No: 2005.111.004.02). The authors Faik Ahmet Ayaz and Huseyin Inceer also greatly appreciate the support from the Council of Higher Education of Turkey (CoHE). This work was also financially supported by the Ministry of Education, Youth and Sports of the Czech Republic through the National Program of Sustainability (grant no. LO1204)

Comparison of Phenolics and Phenolic Acid Profiles in Conjunction with Oxygen Radical Absorbing Capacity (ORAC) in Berries of Vaccinium arctostaphylos L. and V. myrtillus L.

Caucasian blueberry (Vaccinium arctostaphylos L.) and bilberry (V. myrtillus L.), both native to Turkey, were evaluated for their total phenolics (TP) and anthocyanin (TAC) contents. Individual compositions of free phenolic acids and phenolic acids liberated from ester and glycoside forms were analyzed using UPLC-MS/MS. Berry extracts of each species were separated into three different fractions (sugar/acid, polyphenolic and anthocyanin) by solid phase extraction (SPE). The anthocyanin fractions of each species had the highest level of TP and TAC contents and ORAC values. Each species contained 10 phenolic acids representing each fraction, but in different quantities. The phenolic acids liberated from the esters and glycoside forms were caffeic acid and p-coumaric acid. The fractions containing phenolic acids liberated from ester and glycoside forms had a higher antioxidant capacity than that from free phenolic acids. The data suggest that both berries have potential as good dietary sources of phenolic antioxidants