Association of dyslipidemia in second trimester of pregnancy with preeclampsia

Background: The study aimed to determine whether dyslipidemia in the second trimester of pregnancy is associated with pre-eclampsia and to investigate and compare the levels of serum lipids among preeclampsia and normal pregnant women.Methods: This prospective cohort study was done between March 2016 to February 2017. Fasting lipid profile was taken for about 200 selected antenatal women between 18-20 weeks of gestation. The patients who developed pre-eclampsia were grouped as preeclampsia group and the rest of the patients were grouped as normal group. The maternal characteristics compared between two groups were age, parity, BMI and socioeconomic class according to modified Kuppuswamy scale.Results: There is statistical significance among the two groups with respect to BMI and parity whereas no significance with age distribution. Mean total cholesterol in Preeclampsia was 240.24±46.63 mg/dl and normal pregnancy was 186.10±28.02 mg/dl. Mean HDL in preeclampsia was 47.49±4.40mg/dl and normal pregnancy was 52.11±8.918 mg/dl. Mean LDL in preeclampsia was 140.43±36.92 mg/ dl and normal pregnancy was 94.6±24.5 mg/dl. Mean VLDL in preeclampsia was 64.48±15.76mg/dl and in normal pregnancy was 33.54±9.38 mg/dl, Mean Triglycerides in preeclampsia was 291.95±82.33 mg/dl and normal pregnancy was 166.78±48.83 mg/dl. Total cholesterol, Low density lipoprotein, very low-density lipoprotein, triglycerides were increased in preeclampsia when compared to normal pregnancy, which is statistically significant.Conclusions: Detecting dyslipidemia before 20 weeks of gestation helps to recognise pregnancies at high risk for preeclampsia and to detect and treat the disease earlier for a better maternal and perinatal outcome

Perinatal outcome in relation to maternal glycaemic control in gestational diabetes mellitus

Background: Early diagnosis and treatment of gestational diabetes with adequate antenatal care are essential to reduce the adverse neonatal outcomes.Methods: All selected women were subjected to a detailed history taking comprising of age, parity, prepregnant body weight, medication history, family history, medical history, detailed obstetric history. Then they were subjected to clinical examination and routine laboratory investigations. Antenatal women were diagnosed with GDM, when 75gm GCT ≥140mg/dl based on DIPSI criteria. Fasting and postprandial blood sugars (2hours) were done and if FBS and PPBS were normal, the patient was labelled as GDM on meal plan. If fasting > 96 mg/dl and 2-hr postprandial blood sugar >121 mg/dl, Inj.insulin was started. Patients were managed according to the FBS and PPBS values throughout antenatal period and the perinatal outcomes were studied.Results: Among 150 patients, multiparous women constitute 65.3% and primi 34.7%. 60% of the patients were in age group of 26 to 30 years.59.3% of patients were in BMI of range 25 to 30.Family history of diabetes was present in 24.7% of the patients. Previous history of GDM was present in 27.3% of cases.98% of GDM were detected in 2nd trimester. The percentage of cases presented with macrosomia was 8.7%, RDS 3.3%, hypoglycaemia 6%, hyperbilirubinemia 3.3%, IUD 0.7%, perinatal injury 1.3%, NICU admission requiring ≥3 days of admission was 12%.Conclusions: There is association of elevated FBS and PPBS values with occurrence of adverse perinatal outcome

Binding of activated isoniazid with acetyl-CoA carboxylase from Mycobacterium tuberculosis

AccD6 (acetyl coenzyme A (CoA) carboxylase), plays an important role in mycolic acid synthesis of Mycobacterium tuberculosis (Mtb). Induced gene expression by isoniazid (isonicotinylhydrazine - INH), anti-tuberculosis drug) shows the expression of accD6. It is our interest to study the binding of activated INH with the AccD6 model using molecular docking procedures. The study predicts a primary binding site for activated INH (isonicotinyl acyl radical) in AccD6 as a potential target

Online toll payment system using android application

Now a day’s android application are emerging popular day by day.Online toll payment system will be very useful for the passengers and also the payment can be done in online. The toll payment system consists of an application that generates QR-Scanner code that will be displayed on the mobile device after the payment is done. The application also gives notification to the passenger before 2km or 3km before reaching the toll booth. The generated QR code will be scanned through a camera mobile device which is connected to the mySql server. The code generated is compared with server data and will be verified

Approximation theory in up-algebras based on intuitionistic fuzzy sets

This paper discusses the upper and lower approximations of Atanassov intuitionistic fuzzy sets in crisp and fuzzy approximation spaces in which both constructive and axiomatic approaches are used. In the constructive approach, concepts of intuitionistic fuzzy sets are defined, properties of intuitionistic fuzzy approximation operators are examined. Different classes of intuitionistic fuzzy set algebras are obtained from different types of fuzzy relations. In the axiomatic approach, an operator-oriented characterization of intuitionistic fuzzy sets is proposed

A nutrigenetic approach to investigate the relationship between metabolic traits and vitamin D status in an Asian Indian population

Studies in Asian Indians have examined the association of metabolic traits with vitamin D status. However, findings have been quite inconsistent. Hence, we aimed to explore the relationship between metabolic traits and 25-hydroxyvitamin D [25(OH)D] concentrations. We investigate whether this relationship was modified by lifestyle factors using a nutrigenetic approach in 545 Asian Indians randomly selected from the Chennai Urban Rural Epidemiology Study (219 normal glucose tolerant individuals, 151 with pre-diabetes and 175 individuals with type 2 diabetes). A metabolic genetic risk score (GRS) was developed using five common metabolic disease-related genetic variants. There was a significant interaction between metabolic GRS and carbohydrate intake (energy%) on 25(OH)D (Pinteraction = 0.047). Individuals consuming a low carbohydrate diet (≤62%) and those having lesser number of metabolic risk alleles (GRS ≤ 1) had significantly higher levels of 25(OH)D (p = 0.033). Conversely, individuals consuming a high carbohydrate diet despite having lesser number of risk alleles did not show a significant increase in 25(OH)D (p = 0.662). In summary, our findings show that individuals carrying a smaller number of metabolic risk alleles are likely to have higher 25(OH)D levels if they consume a low carbohydrate diet. These data support the current dietary carbohydrate recommendations of 50%–60% energy suggesting that reduced metabolic genetic risk increases 25(OH)D

Studies on the Impact of Semi-ripe along with Seedless Papaya (Carica papaya) Fruits Pulp and Seeds Extract on the Level of Sialic acid in male Albino Rat Reproductive tissue

The present research investigation intends at investigating the effect of on Sialic acid components in male albino rats of an aqueous extract of seeded papaya fruit pulp and seed extract specified as a combined dose and seedless variety papaya fruit pulp extract. The primary objective of the current research investigation was to evaluate the effects that occurred on male albino Wistar rats of semi-ripe fruit pulp extract from Carica papaya (fruits with and without seeds). The development of spermatozoa's ability to fertilise, the coating of spermatozoa with specific antigens, and changes in their membrane surface are all related to Sialic acid. Less spermatozoa cells in the testicular compartments and fewer of these cells are indicated by the decrease in Sialic acid contents in the testis and epididymis of both experimental groups

Evidence for the association between FTO gene variants and vitamin B12 concentrations in an Asian Indian population

Background Low vitamin B12 concentrations have been associated with major clinical outcomes, including adiposity, in Indian populations. The Fat mass and obesity-associated gene (FTO) is an established obesity-susceptibility locus; however, it remains unknown whether it influences vitamin B12 status. Hence, we investigated the association of two previously studied FTO polymorphisms with vitamin B12 concentrations and metabolic disease-related outcomes and examined whether these associations were modified by dietary factors and physical activity. Methods A total of 176 individuals with type 2 diabetes, 152 with pre-diabetes, and 220 normal glucose-tolerant individuals were randomly selected from the Chennai Urban Rural Epidemiology Study. Anthropometric, clinical, and biochemical investigations, which included body mass index (BMI), waist circumference, vitamin B12, homocysteine, and folic acid were measured. A validated food frequency questionnaire was used for dietary assessment and self-reported physical activity measures were collected. An unweighted genetic risk score (GRS) was calculated for two FTO single-nucleotide polymorphisms (rs8050136 and rs2388405) by summation of the number of risk alleles for obesity. Interaction analyses were performed by including the interaction terms in the regression model. Results The GRS was significantly associated with increased BMI (P = 0.009) and risk of obesity (P = 0.023). Individuals carrying more than one risk allele for the GRS had 13.13% lower vitamin B12 concentrations, compared to individuals carrying zero risk alleles (P = 0.018). No associations between the GRS and folic acid and homocysteine concentrations were observed. Furthermore, no statistically significant GRS-diet or GRS-physical activity interactions with vitamin B12, folic acid, homocysteine or metabolic-disease outcomes were observed. Conclusion The study shows for the first time that a genetic risk score using two FTO SNPs is associated with lower vitamin B12 concentrations; however, we did not identify any evidence for the influence of lifestyle factors on this association. Further replication studies in larger cohorts are warranted to investigate the association between the GRS and vitamin B12 concentrations

Paradoxical tuberculosis immune reconstitution inflammatory syndrome (TB-IRIS) in HIV patients with culture confirmed pulmonary tuberculosis in India and the potential role of IL-6 in prediction

Background: The incidence, manifestations, outcome and clinical predictors of paradoxical TB-IRIS in patients with HIV and culture confirmed pulmonary tuberculosis (PTB) in India have not been studied prospectively. Methods: HIV+ patients with culture confirmed PTB started on anti-tuberculosis therapy (ATT) were followed prospectively after anti-retroviral therapy (ART) initiation. Established criteria for IRIS diagnosis were used including decline in plasma HIV RNA at IRIS event. Pre-ART plasma levels of interleukin (IL)-6 and C-reactive protein (CRP) were measured. Univariate and multivariate logistic regression models were used to evaluate associations between baseline variables and IRIS. Results: Of 57 patients enrolled, 48 had complete follow up data. Median ATT-ART interval was 28 days (interquartile range, IQR 14–47). IRIS events occurred in 26 patients (54.2%) at a median of 11 days (IQR: 7–16) after ART initiation. Corticosteroids were required for treatment of most IRIS events that resolved within a median of 13 days (IQR: 9–23). Two patients died due to CNS TB-IRIS. Lower CD4+ T-cell counts, higher plasma HIV RNA levels, lower CD4/CD8 ratio, lower hemoglobin, shorter ATT to ART interval, extra-pulmonary or miliary TB and higher plasma IL-6 and CRP levels at baseline were associated with paradoxical TB-IRIS in the univariate analysis. Shorter ATT to ART interval, lower hemoglobin and higher IL-6 and CRP levels remained significant in the multivariate analysis. Conclusion: Paradoxical TB–IRIS frequently complicates HIV-TB therapy in India. IL-6 and CRP may assist in predicting IRIS events and serve as potential targets for immune interventions

Mycobacterial antigen driven activation of CD14++ CD16-monocytes is a predictor of tuberculosis-associated immune reconstitution inflammatory syndrome

Paradoxical tuberculosis-associated immune reconstitution inflammatory syndrome (TB-IRIS) is an aberrant inflammatory response occurring in a subset of TB-HIV co-infected patients initiating anti-retroviral therapy (ART). Here, we examined monocyte activation by prospectively quantitating pro-inflammatory plasma markers and monocyte subsets in TB-HIV co-infected patients from a South Indian cohort at baseline and following ART initiation at the time of IRIS, or at equivalent time points in non-IRIS controls. Pro-inflammatory biomarkers of innate and myeloid cell activation were increased in plasma of IRIS patients pre-ART and at the time of IRIS; this association was confirmed in a second cohort in South Africa. Increased expression of these markers correlated with elevated antigen load as measured by higher sputum culture grade and shorter duration of anti-TB therapy. Phenotypic analysis revealed the frequency of CD14++CD16− monocytes was an independent predictor of TB-IRIS, and was closely associated with plasma levels of CRP, TNF, IL-6 and tissue factor during IRIS. In addition, production of inflammatory cytokines by monocytes was higher in IRIS patients compared to controls pre-ART. These data point to a major role of mycobacterial antigen load and myeloid cell hyperactivation in the pathogenesis of TB-IRIS, and implicate monocytes and monocyte-derived cytokines as potential targets for TB-IRIS prevention or treatment