Regulation of miR394 in Response to Fusarium oxysporum f. sp. cepae (FOC) Infection in Garlic (Allium sativum L)

MicroRNAs (miRNAs) are a class of post transcriptional regulators that negatively regulate gene expression through target mRNA cleavage or translational inhibition and play important roles in plant development and stress response. In the present study, 6 conserved miRNAs from garlic (Allium sativum L.) were analysed to identify differentially expressed miRNAs in response to Fusarium oxysporum f. sp. cepae (FOC) infection. Stem-loop RT-PCR revealed that miR394 is significantly induced in garlic seedlings post treatment with FOC for 72 h. The induction of miR394 expression during FOC infection was restricted to the basal stem plate tissue, the primary site of infection. Garlic miR394 was also upregulated by exogenous application of jasmonic acid. Two putative targets of miR394 encoding F-box domain and cytochrome P450 (CYP450) family proteins were predicted and verified using 5’ RLM-RACE (RNA ligase mediated rapid amplification of cDNA ends) assay. Quantitative RT-PCR showed that the transcript levels of the predicted targets were significantly reduced in garlic plants exposed to FOC. When garlic cultivars with variable sensitivity to FOC were exposed to the pathogen, an upregulation of miR394 and down regulation of the targets were observed in both varieties. However, the expression pattern was delayed in the resistant genotypes. These results suggest that miR394 functions in negative modulation of FOC resistance and the difference in timing and levels of expression in variable genotypes could be examined as markers for selection of FOC resistant garlic cultivars

Isolation and characterization of gene encoding vicilin (7S) protein from cDNA clones of immature seeds of pigeon pea [<i style="">Cajanus cajan</i> (L.) Millsp.]

32-36cDNA library was constructed in lgt 11 expression vector from mRNA of immature seeds of pigeonpea.&nbsp; This cDNA was screened with specific non-radioactive, DIG-labelled heterologous pea vicilin cDNA probe (pRC-758). The positive vicilin (7S) encoding cDNA clones were isolated. One of the vicilin cDNA clones showed insert size of ~1.3 Kb, when analyzed by PCR using lgt 11 forward and reverse primers. This PCR product was subcloned in pUC-18 vector for confirmation of gene and product by Southern and Western hybridizations, respectively. The clone was named as pSL-1 and sequenced with M13 universal forward and reverse sequencing primers. The partial nucleotide sequence (1341 bp) has been indexed in NCBI gene bank with accession number AF348366. The complete gene has 1417 base pairs with 972 bp coding sequence. Hence, the predicted polypeptide chain of this gene was determined, which contained 323 amino acids. After post translation modification, the predicted polypeptide has 310 amino acids long with approximately mol wt of 34.1 kDa. This gene encoding vicilin (7S) protein provides the basic understanding of the gene structure of pigeonpea storage proteins

Abstracts of National Conference on Research and Developments in Material Processing, Modelling and Characterization 2020

This book presents the abstracts of the papers presented to the Online National Conference on Research and Developments in Material Processing, Modelling and Characterization 2020 (RDMPMC-2020) held on 26th and 27th August 2020 organized by the Department of Metallurgical and Materials Science in Association with the Department of Production and Industrial Engineering, National Institute of Technology Jamshedpur, Jharkhand, India. Conference Title: National Conference on Research and Developments in Material Processing, Modelling and Characterization 2020Conference Acronym: RDMPMC-2020Conference Date: 26–27 August 2020Conference Location: Online (Virtual Mode)Conference Organizer: Department of Metallurgical and Materials Engineering, National Institute of Technology JamshedpurCo-organizer: Department of Production and Industrial Engineering, National Institute of Technology Jamshedpur, Jharkhand, IndiaConference Sponsor: TEQIP-