2 research outputs found
Biomolecular Analysis and Biological Tissue Diagnostics by Electrospray Ionization with a Metal Wire Inserted Gel-Loading Tip
A metal
wire-inserted disposable gel-loading tip was examined as
an electrospray emitter. Its performance was similar to that of conventional
electrospray ionization (ESI) with a relatively low flow rate (∼100
nL/min) and without the need for solvent pumps. It was also used as
an emitter for solid probe-assisted ESI (SPA-ESI) (e.g., biofluid
was sampled from the biological tissue by a needle and was inserted
into the solvent-preloaded gel-loading tip). Selective detection of
lipids and proteins, such α and β chains of hemoglobin
could be accomplished by choosing appropriate solvents. A suitable
protocol for cancer diagnosis was established by this method. A good
figure of merit of this method is its applicability to biological
tissue diagnostics with high cost efficiency and on a disposable basis
Development of Sheath-Flow Probe Electrospray Ionization Mass Spectrometry and Its Application to Real Time Pesticide Analysis
For
the real time and direct analysis of chemical constituents
from living beings and dry sample, sheath-flow probe electrospray
ionization mass spectrometry (SF-PESI-MS) has been newly developed.
The components from dry or semidry biological tissues can be extracted
using the solvent and picked up by the needle for electrospray. This
technique was applied to real-time pesticide analysis of living plants.
The results have been validated with that of a well-known system,
liquid extraction surface analysis mass spectrometry (LESA-MS). It
is demonstrated that SF-PESI-MS can produce reasonable ionization
efficiency, which is confirmed by LESA-MS