MOESM1 of Host, pathogen and the environment: the case of Macrobrachium rosenbergii, Vibrio parahaemolyticus and magnesium

Additional file 1. Fasta sequence of the Vibrio parahaemolyticus GbpA protein

Maximum likelihood-based phylogenetic analysis generated using MEGA X software (Bootstrap: 1000) for total cDNA sequences.

Sample groups: M. rosenbergii and P. monodon. Genes: C-type Lectin, HMGB, STAT, ALF3 (ALF), and ATPase 8/6 (ATPase).</p

Display of genetic differentiation between different genes of total cDNA sequences.

Mr: M. rosenbergii; Pm: P. monodon. Genes: C-type Lectin (CTL), HMGB, STAT, ALF3 (ALF), ATPase 8/6 (ATP).</p

Heavy ion physics with the ATLAS detector

The ATLAS experiment at the Large Hadron Collider has undertaken a broad physics program to probe and characterize the hot nuclear matter created in relativistic lead-lead collisions. This talk presents recent results on production of jet, electroweak bosons and quarkonium, electromagnetic processes in ultra-peripheral collisions, and bulk particle collectivity from Pb+Pb and p+Pb collisions

Neighbour-joining tree showing all available DNA barcodes for species in family Hipposideridae reported from Peninsular Malaysia.

<p>The percentage of pseudoreplicate trees (≥70%) in which the DNA barcodes clustered together in the bootstrap test (500 pseudoreplicates) are shown above the branches. Abbreviation as follows: PM = Peninsular Malaysia, VN = Vietnam, BN = Borneo (including Sabah & Sarawak of East Malaysia, Brunei and Kalimantan Indonesia), TH = Thailand, LA = Laos, SM = Sumatera Indonesia, CH = China, CM = Cambodia.</p

Neighbour-joining tree showing all available DNA barcodes for species in families Emballonuridae, Megadermatidae, Molossidae and Nycteridae reported from Peninsular Malaysia.

<p>The percentage of pseudoreplicate trees (≥70%) in which the DNA barcodes clustered together in the bootstrap test (500 pseudoreplicates) are shown above the branches. Abbreviation as follows: PM = Peninsular Malaysia, VN = Vietnam, BN = Borneo (including Sabah & Sarawak of East Malaysia, Brunei and Kalimantan Indonesia), TH = Thailand, LA = Laos, SM = Sumatera Indonesia, CH = China.</p

Bat species with recent (dated during or after the year 2000) and old (dated before year 2000) records from Peninsular Malaysia.

<p>Bat species with recent (dated during or after the year 2000) and old (dated before year 2000) records from Peninsular Malaysia.</p

Limited dispersal and geographic barriers cause population differentiation and structuring in <i>Begonia maxwelliana</i> at both large and small scales

<p><b><i>Background</i></b>: Genetic divergence is one of the key processes in speciation. In the Begoniaceae, genetic divergence caused by limited gene flow may explain its high species diversity and endemicity. This hypothesis has been supported by past genetic work but there is a lack of empirical studies on the causes of limited gene flow.</p> <p><b><i>Aim</i></b>: To identify the causes of limited gene flow in <i>Begonia</i>.</p> <p><b><i>Methods</i></b>: We examined the genetic structure among the populations of <i>Begonia maxwelliana</i> at the macro- and micro-spatial scales using microsatellites, measured seed dispersal range and observed flowering phenology.</p> <p><b><i>Results</i></b>: Population differentiation and structuring were detected at both the macro- and micro-scales. Dispersal range was short, and all populations showed similar reproductive behaviour.</p> <p><b><i>Conclusions</i></b>: The strong population differentiation and structuring among the populations studied imply that they are evolutionarily significant units and possible candidates for speciation. Geographical barriers and limited seed dispersal restrict gene flow in the populations, and these factors may be responsible for the rapid speciation and large diversity in the family.</p

In-Depth Tanscriptomic Analysis on Giant Freshwater Prawns

<div><p>Gene discovery in the Malaysian giant freshwater prawn (<i>Macrobrachium rosenbergii</i>) has been limited to small scale data collection, despite great interest in various research fields related to the commercial significance of this species. Next generation sequencing technologies that have been developed recently and enabled whole transcriptome sequencing (RNA-seq), have allowed generation of large scale functional genomics data sets in a shorter time than was previously possible. Using this technology, transcriptome sequencing of three tissue types: hepatopancreas, gill and muscle, has been undertaken to generate functional genomics data for <i>M. rosenbergii</i> at a massive scale. <i>De novo</i> assembly of 75-bp paired end Ilumina reads has generated 102,230 unigenes. Sequence homology search and <i>in silico</i> prediction have identified known and novel protein coding candidate genes (∼24%), non-coding RNA, and repetitive elements in the transcriptome. Potential markers consisting of simple sequence repeats associated with known protein coding genes have been successfully identified. Using KEGG pathway enrichment, differentially expressed genes in different tissues were systematically represented. The functions of gill and hepatopancreas in the context of neuroactive regulation, metabolism, reproduction, environmental stress and disease responses are described and support relevant experimental studies conducted previously in <i>M. rosenbergii</i> and other crustaceans. This large scale gene discovery represents the most extensive transcriptome data for freshwater prawn. Comparison with model organisms has paved the path to address the possible conserved biological entities shared between vertebrates and crustaceans. The functional genomics resources generated from this study provide the basis for constructing hypotheses for future molecular research in the freshwater shrimp.</p></div

Проект кафе "Новое время" на 54 места

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