9 research outputs found
Development of HankelâSVD hybrid technique for multiple noise removal from PD signature
No full textHANKELâEMâSVD: a hybrid data dropout estimation technique for high voltage partial discharge signals
No full textQTL Mapping of Adventitious Root Formation under Flooding Conditions in Tropical Maize (Zea mays L.) Seedlings
No full textChanges in morphological and physiological traits and stress-related enzyme activities of green bean (Phaseolus vulgaris L.) genotypes in response to waterlogging stress and recovery treatment
No full textPlant regeneration from cotyledonary explants of Eucalyptus camaldulensis dehn and histological study of organogenesis in vitro
No full textThe present work aimed at regenerating plants of Eucalyptus camaldulensis from the cotyledonary explants and describing the anatomy of the tissues during callogenesis and organogenesis processes, in order to determine the origin of the buds. The cotyledonary leaves of E. camaldulensis were cultured in Murashige and Skoog (MS), WPM and JADS media supplemented with 2.7 ”M NAA and 4.44 ”M BAP. The best results for bud regeneration were obtained on MS and WPM media (57.5 and 55% of calluses formed buds, respectively). Shoot elongation and rooting (80%) were obtained on MS/2 medium (with half-strength salt concentration) with 0.2% activated charcoal. Acclimatization was performed in the growth chamber for 48 h and then the plants were transferred to a soil:vermiculite mixture and cultured in a greenhouse. Histological studies revealed that the callogenesis initiated in palisade parenchyma cells and that the adventitious buds were formed from the calluses, indicating indirect organogenesis.<br>Este trabalho teve como objetivo a obtenção de plantas de Eucalyptus camaldulensis a partir de folhas cotiledonares e o estudo da anatomia dos tecidos durante a calogĂȘnese e organogĂȘnese para determinar a origem das gemas. Folhas cotiledonares foram cultivadas em meios de cultura MS, WPM e JADS suplementados com 2,7 ”M de ANA e 4,44 ”M de BAP. Os melhores resultados para a regeneração de gemas foram obtidos com os meios MS e WPM. Para o alongamento e enraizamento, o meio de cultura MS/2 contendo 0,2% de carvĂŁo ativado apresentou-se eficiente para ambas as etapas. A aclimatização foi realizada mediante a abertura dos frascos na sala de crescimento por 48 horas, seguido da transferĂȘncia para casa-de-vegetação com nebulização intermitente. Estudos histolĂłgicos foram conduzidos e revelaram que a calogĂȘnese teve inĂcio nas cĂ©lulas do parĂȘnquima paliçådico e que as gemas adventĂcias formaram-se a partir dos calos, indicando a organogĂȘnese indireta
