Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring.

The new and rapid advancement in the complexity of biologics drug discovery has been driven by a deeper understanding of biological systems combined with innovative new therapeutic modalities, paving the way to breakthrough therapies for previously intractable diseases. These exciting times in biomedical innovation require the development of novel technologies to facilitate the sophisticated, multifaceted, high-paced workflows necessary to support modern large molecule drug discovery. A high-level aspiration is a true integration of "lab-on-a-chip" methods that vastly miniaturize cellulmical experiments could transform the speed, cost, and success of multiple workstreams in biologics development. Several microscale bioprocess technologies have been established that incrementally address these needs, yet each is inflexibly designed for a very specific process thus limiting an integrated holistic application. A more fully integrated nanoscale approach that incorporates manipulation, culture, analytics, and traceable digital record keeping of thousands of single cells in a relevant nanoenvironment would be a transformative technology capable of keeping pace with today's rapid and complex drug discovery demands. The recent advent of optical manipulation of cells using light-induced electrokinetics with micro- and nanoscale cell culture is poised to revolutionize both fundamental and applied biological research. In this review, we summarize the current state of the art for optical manipulation techniques and discuss emerging biological applications of this technology. In particular, we focus on promising prospects for drug discovery workflows, including antibody discovery, bioassay development, antibody engineering, and cell line development, which are enabled by the automation and industrialization of an integrated optoelectronic single-cell manipulation and culture platform. Continued development of such platforms will be well positioned to overcome many of the challenges currently associated with fragmented, low-throughput bioprocess workflows in biopharma and life science research

Self-efficacy care model helps self-care efficacy and physical activity in older people with hip fracture

1.The self-efficacy Care Model (SCM) nursing intervention can significantly improve self-care efficacy of older patients after receiving hip fracture surgery. 2.The self-efficacy Care Model (SCM) nursing intervention can significantly increase physical activity in older patients with femoral intertrochanteric fracture receiving hip fracture surgery

Activities of daily living and quality of life of older patients undergoing hip fracture surgery

The study aimed to evaluate the relationships of activities of daily living and quality of life in Chinese older patients undergoing hip fracture surgery. The older and longer hospital stays of the patients, the lower the daily activity and physical quality of life

Practices of Market Making for Sustaining Electronic Auction

This article examines how technology may be used continuously in organizations. Particularly, it investigates the organizing practices that support continuous technology-use. Exploring such organizing practices is meaningful because they could sustain ongoing organizational innovation. A field study on the enterprise application of e-auction (electronic auction, or otherwise known as online reverse auction and electronic bidding) is used to illustrate how a marketplace is effectively maintained over a span of seven years. Our findings identify a set of market-making practices and its principles that underscore sustained use of e-auction. This research contributes to literature on technology-use and adoption, as well as adds to studies on the electronic marketplace. It also offers practical lessons to implementers who seek to adopt the e-auction to achieve significant cost-savings and streamline the supply chain

Patients\u27 coping process of having acute myocardial infraction attack and receiving urgent percutaneous coronary interventions (PCIs): A grounded theory study

Session presented on Monday, November 9, 2015 and Tuesday, November 10, 2015: Background: Cardiovascular diseases represent the second cause of death in Taiwan, resulting in annually 4,000 to 5,000 people die by AMI. However, limited studies focus on investigating AMI patients\u27 coping process of PCIs. Objective: This study explored coping process of AMI patients underwent emergent PCIs. Methods: A total of 29 AMI patients receiving emergent PCIs were recruited from 2 hospitals in Taiwan and participated in the semi-structured interviews. Data were analysed through a multi-step synthesized grounded theory analysis developed by the researchers. Methods of asking questions, reviewing references, constant comparison, and theoretical sampling were utilised throughout the data collection process for achieving theoretical saturation and research integrity. Results: Hypertension, diabetes mellitus, hyperlipidemia, hardworking, huge stress, no exercise, poor life style, were the high risks to induce AMI. When AMI attacks, it causes physical, psychological, social, and economic problems for patients and their families. Blank mind, dying, powerless, regretful, goodbye, and helpless were patients\u27 experiences of AMI attack. Pain, nervous, unconscious, difficult times, or no idea, were their perceptions of receiving emergent PCIs. AMI patients regretted to live inappropriately and tried to have good life styles in getting healthy back. They used positive coping strategies, such as life style change, medication compliance, and exercise, to face AMI. Conclusions: Patients only perceived disease seriousness as they had AMI attack. We suggest that early prevention and enough education of AMI diseases are necessary in clinical practice and community care. However, due to small number of participants, we suggest further study is needed

The development and psychometric testing of the Cognitive of Information Literacy Inventory (CILI)

Purpose: This is two-year research project, one of the aims was to develop and test two instruments, including the CILI and attitude toward information literacy inventory (ATILI). This study was to develop and test validity and reliability of the CILI to measure the cognitive of information literacy of nursing students. Methods: This study was conducted in 3 phases. In Phase 1, based on a review of the literature, the researchers developed an instrument to measure CILI. In Phase 2, two rounds of the Delphi study were conducted to determine the content validity of the instrument. In Phase 3, a convenience sample of 983 nursing students was recruited to test the validity and reliability of the CILI. Exploratory factor analysis was used to determine the factor structure of the inventory. Results: The resulting CILI consists of 14 items. Construct validity was determined by exploratory factor analysis using varimax rotation. The result revealed that KMO was greater than .90 and Bartlett\u27s test of Sphericity showed significant, these information indicates an appropriateness to do factor analysis. Factor loading greater than .40 was selected, two important categories were revealed, including information management and databases search , the total explained variance was 58.28%. The Cronbach\u27s alpha level of the inventory was 0.92. Conclusions: The CILI has good psychometric properties and can be used to understand the cognitive of information literacy for nursing students. Faculty members can assess nursing students\u27 cognitive of information literacy to design appropriate lesson plans and implement appropriate teaching strategies for nursing students in order foster the growth of lifelong learning abilities

7-Dimethyl­amino-2-phenyl-1,2,4-triazolo[1,5-a][1,3,5]triazin-5-amine methanol solvate1

7-Dimethyl­amino-2-phenyl-1,2,4-triazolo[1,5-a][1,3,5]triazin-5-amine crystallized with one mol­ecule of methanol to give the title compound, C12H13N7·CH3OH. The triazolo[1,5-a][1,3,5]triazine heterocyclic core is essentially planar as are both amino groups that are involved in π-electron delocalization with the triazolo[1,5-a][1,3,5]triazine nucleus. The methyl groups of the dimethyl­amino fragment are involved in the formation of weak intra­molecular C—H⋯N hydrogen bonds with the N atoms of the heterocyclic system. The crystal packing is stabilized by inter­molecular N—H⋯N hydrogen bonds between the triazolo[1,5-a][1,3,5]triazine mol­ecules. The methanol solvent mol­ecule also participates in the formation of the crystal structure via inter­molecular O—H⋯N, N—H⋯O and weak C—H⋯O hydrogen bonds, linking the layers of triazolo[1,5-a][1,3,5]triazine mol­ecules

Seabed gas emissions and submarine landslides off SW Taiwan

Methane emissions out of the seabed could seriously affect Earthâs climate and are usually associated with the dissociation of gas hydrates stored in marine sediments on the continental margins. Spatially, gas emissions out of the seafloor are not evenly distributed in continental margins. Gas emissions out of the seabed generally occur through submarine mud volcanoes and gas seeps. To understand the seabed gas emissions off SW Taiwan, we investigate the distributions of active submarine mud volcanoes, gas seeps, and gas plumes off SW Taiwan. We examine all of the available sub-bottom profiler and EK echo sounder data. We identified 19 submarine mud volcanoes, 220 gas seeps, and 295 gas plumes. The gas emissions are generally distributed at the crests of mud diapiric ridges. Most of the active mud volcanoes and gas seeps cluster at the KASMVG (Kaoping submarine mud volcanoes group) area. We speculate that the intensive mud volcanism and gas seepage at the KASMVG area are ascribed to submarine channel erosion along the continental slope base. The erosion causes a deep V-shaped channel and a steep BSR (Bottom-Simulating Reflector) slope curve across the continental margin. The upward migration rate of free gas beneath the BSR is thus increased and intensifies mud volcanism and gas seepage at the KASMVG area. The gas seeps can reduce the slope stability and generate small-scale slides. The development of mud volcanoes in an area could effectively disturb the seabed morphology so that large-scale submarine landslides cannot easily happen