Developement And Evaluation Of A Nasba System For The Diagnosis Of Cholera Using Elisa And Biosensor Methods

Taun (kolera) ialah penyakit ciri-birit yang disebabkan Vibrio cholerae. Cholera is a diarrheal disease caused by Vibrio cholerae. Cholera is potentially lethal if not diagnosed on time

Development And Evaluation Of A Nasba System For The Diagnosis Of Cholera Using Elisa And Biosensor Methods [RA644.C3 L481 2008 f rb].

Taun (kolera) ialah penyakit ciri-birit yang disebabkan Vibrio cholerae. Taun boleh membawa maut jika tidak dikesan dengan serta-merta. Cholera is a diarrheal disease caused by Vibrio cholerae. Cholera is potentially lethal if not diagnosed on time

The widths of quarkonia in quark gluon plasma

Recent lattice calculations showed that the quarkonia will survive beyond the phase transition temperature, and will dissolve at different temperatures depending on the type of the quarkonium. In this work, we calculate the thermal width of the quarkonium at finite temperature before it dissolves into open heavy quarks. The input of the calculation are the parton quarkonium dissociation cross section to NLO in QCD, the quarkonium wave function in a temperature-dependent potential from lattice QCD, and a thermal distribution of partons with thermal masses. We find that for the J/psi, the total thermal width above 1.4 Tc becomes larger than 100 to 250 MeV, depending on the effective thermal masses of the quark and gluon, which we take between 400 to 600 MeV. Such a width corresponds to an effective dissociation cross section by gluons between 1.5 to 3.5 mb and by quarks 1 to 2 mb at 1.4 Tc. However, at similar temperatures, we find a much smaller thermal width and effective cross section for the upsilon.Comment: 7 pages, 13 figures, 2 tables, version to be published in Phys. Rev.

A nanoplex PCR assay for the rapid detection of vancomycin and bifunctional aminoglycoside resistance genes in Enterococcus species

<p>Abstract</p> <p>Background</p> <p>Enterococci have emerged as a significant cause of nosocomial infections in many parts of the world over the last decade. The most common enterococci strains present in clinical isolates are <it>E. faecalis </it>and <it>E. faecium </it>which have acquired resistant to either gentamicin or vancomycin. The conventional culture test takes 2–5 days to yield complete information of the organism and its antibiotic sensitivity pattern. Hence our present study was focused on developing a nanoplex PCR assay for the rapid detection of vancomycin and bifunctional aminoglycoside resistant enterococci (V-BiA-RE). This assay simultaneously detects 8 genes namely 16S rRNA of <it>Enterococcus </it>genus, <it>ddl </it>of <it>E. faecalis </it>and <it>E. faecium</it>, <it>aac</it>A-<it>aph</it>D that encodes high level gentamicin resistance (HLGR), multilevel vancomycin resistant genotypes such as <it>van</it>A, <it>van</it>B, <it>van</it>C and <it>van</it>D and one internal control gene.</p> <p>Results</p> <p>Unique and specific primer pairs were designed to amplify the 8 genes. The specificity of the primers was confirmed by DNA sequencing of the nanoplex PCR products and BLAST analysis. The sensitivity and specificity of V-BiA-RE nanoplex PCR assay was evaluated against the conventional culture method. The analytical sensitivity of the assay was found to be 1 ng at the DNA level while the analytical specificity was evaluated with 43 reference enterococci and non-enterococcal strains and was found to be 100%. The diagnostic accuracy was determined using 159 clinical specimens, which showed that 97% of the clinical isolates belonged to <it>E. faecalis</it>, of which 26% showed the HLGR genotype, but none were vancomycin resistant. The presence of an internal control in the V-BiA-RE nanoplex PCR assay helped us to rule out false negative cases.</p> <p>Conclusion</p> <p>The nanoplex PCR assay is robust and can give results within 4 hours about the 8 genes that are essential for the identification of the most common <it>Enterococcus </it>spp. and their antibiotic sensitivity pattern. The PCR assay developed in this study can be used as an effective surveillance tool to study the prevalence of enterococci and their antibiotic resistance pattern in hospitals and farm animals.</p

In Situ Measurement of the Junction Temperature of Light Emitting Diodes Using a Flexible Micro Temperature Sensor

This investigation aimed to fabricate a flexible micro resistive temperature sensor to measure the junction temperature of a light emitting diode (LED). The junction temperature is typically measured using a thermal resistance measurement approach. This approach is limited in that no standard regulates the timing of data capture. This work presents a micro temperature sensor that can measure temperature stably and continuously, and has the advantages of being lightweight and able to monitor junction temperatures in real time. Micro-electro-mechanical-systems (MEMS) technologies are employed to minimize the size of a temperature sensor that is constructed on a stainless steel foil substrate (SS-304 with 30 μm thickness). A flexible micro resistive temperature sensor can be fixed between the LED chip and the frame. The junction temperature of the LED can be measured from the linear relationship between the temperature and the resistance. The sensitivity of the micro temperature sensor is 0.059 ± 0.004 Ω/°C. The temperature of the commercial CREE® EZ1000 chip is 119.97 °C when it is thermally stable, as measured using the micro temperature sensor; however, it was 126.9 °C, when measured by thermal resistance measurement. The micro temperature sensor can be used to replace thermal resistance measurement and performs reliably

Sensor Fabrication Method for in Situ Temperature and Humidity Monitoring of Light Emitting Diodes

In this work micro temperature and humidity sensors are fabricated to measure the junction temperature and humidity of light emitting diodes (LED). The junction temperature is frequently measured using thermal resistance measurement technology. The weakness of this method is that the timing of data capture is not regulated by any standard. This investigation develops a device that can stably and continually measure temperature and humidity. The device is light-weight and can monitor junction temperature and humidity in real time. Using micro-electro-mechanical systems (MEMS), this study minimizes the size of the micro temperature and humidity sensors, which are constructed on a stainless steel foil substrate (40 μm-thick SS-304). The micro temperature and humidity sensors can be fixed between the LED chip and frame. The sensitivities of the micro temperature and humidity sensors are 0.06 ± 0.005 (Ω/°C) and 0.033 pF/%RH, respectively

Nickel hydroxide/chemical vapor deposition-grown graphene/nickel hydroxide/nickel foam hybrid electrode for high performance supercapacitors

Rational design of electrode structures has been recognized as an effective strategy to improve the electrochemical performance of electrode materials. Herein, we demonstrate an integrated electrode in which nickel hydroxide (Ni(OH)2) nanosheets are deposited on both sides of chemical vapor deposition-grown graphene on Ni foam, which not only effectively optimizes electrical conductivity of Ni(OH)2, but also accommodates the structural deformation assciated with the large volume change upon cycling. The synthesized Ni(OH)2/graphene/Ni(OH)2/Ni foam electrode exhibits a high specific capacity of 991 C g−1 at a current density of 1 A g−1, which is higher than the theoretical specific capacity of additive sum of Ni(OH)2 and graphene, and retains 95.4% of the initial capacity after 5000 cycles. A hybrid supercapacitor is constructed by using Ni(OH)2/graphene/Ni(OH)2/Ni foam as the positive electrode and activated carbon on Ni foam as the negative electrode, which achieves a maximum energy density of 49.5 W h kg−1 at a power density of 750 W kg−1, and excellent cycling lifespans with 89.3% retention after 10000 cycles at 10 A g−1