Guo1 and "Guo2" in Chinese Temporal System

This paper aims to investigate the subtle nuances of meaning of two Chinese particles “guo1 ” and “guo2 ” as well as their different functions in Chinese temporal system. Two technical terms, “tense ” and “aspect”, in traditional Chinese grammar are reconsidered in terms of the nature of these two concepts and the criteria to distinguish them. It is argued that in traditional Chinese grammar, “tense” and “aspect ” are often mixed up by scholars, which has misled the study of “guo1 ” and “guo2”. Contrast to the traditional theory, this paper argues that “guo1 ” is the marker of the terminative aspect, while “guo2 ” is the marker of the past tense. Moreover, based on the markedness theory, the semantic and functional differences between “guo1 ” and “guo2 ” can be regarded as different usage of the particle “guo ” in the unmarked or the marked sense. 1

A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications.

RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology toward large-scale production of pre-miRNA agents in bacteria, but found the majority of target RNAs were not or negligibly expressed. We thus developed a novel strategy to achieve consistent high-yield biosynthesis of chimeric RNAs carrying various small RNAs (e.g. miRNAs, siRNAs and RNA aptamers), which was based upon an optimal noncoding RNA scaffold (OnRS) derived from tRNA fusion pre-miR-34a (tRNA/mir-34a). Multi-milligrams of chimeric RNAs (e.g. OnRS/miR-124, OnRS/GFP-siRNA, OnRS/Neg (scrambled RNA) and OnRS/MGA (malachite green aptamer)) were readily obtained from 1 l bacterial culture. Deep sequencing analyses revealed that mature miR-124 and target GFP-siRNA were selectively released from chimeric RNAs in human cells. Consequently, OnRS/miR-124 was active in suppressing miR-124 target gene expression and controlling cellular processes, and OnRS/GFP-siRNA was effective in knocking down GFP mRNA levels and fluorescent intensity in ES-2/GFP cells and GFP-transgenic mice. Furthermore, the OnRS/MGA sensor offered a specific strong fluorescence upon binding MG, which was utilized as label-free substrate to accurately determine serum RNase activities in pancreatic cancer patients. These results demonstrate that OnRS-based bioengineering is a common, robust and versatile strategy to assemble various types of small RNAs for broad applications

Model anisotropic quantum Hall states

Model quantum Hall states including Laughlin, Moore-Read and Read-Rezayi states are generalized into appropriate anisotropic form. The generalized states are exact zero-energy eigenstates of corresponding anisotropic two- or multi-body Hamiltonians, and explicitly illustrate the existence of geometric degrees of in the fractional quantum Hall effect. These generalized model quantum Hall states can provide a good description of the quantum Hall system with anisotropic interactions. Some numeric results of these anisotropic quantum Hall states are also presented.Comment: 10 pages, 5 figure