TaqMan analysis of leptin receptor mRNA in shRNA transgenic versus wild-type control mice

<p><b>Copyright information:</b></p><p>Taken from "Single copy shRNA configuration for ubiquitous gene knockdown in mice"</p><p>Nucleic Acids Research 2005;33(7):e67-e67.</p><p>Published online 14 Apr 2005</p><p>PMCID:PMC1079974.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Percentages of leptin receptor mRNA expression in shRNA transgenic mice (grey bars) in comparison with expression levels found in wild-type mice (black bars) ± standard error of the mean are shown. All assays were performed with groups of 4–5 mice at age of 4 months from different organs as indicated

Analysis of shRNA-mediated β-galactisidase silencing at the single cell level

<p><b>Copyright information:</b></p><p>Taken from "Single copy shRNA configuration for ubiquitous gene knockdown in mice"</p><p>Nucleic Acids Research 2005;33(7):e67-e67.</p><p>Published online 14 Apr 2005</p><p>PMCID:PMC1079974.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Histological sections of organs from wt and CAGGS-lacZ transgenic mice, as well as two CAGGS-lacZ/shRNA transgenic animals are shown as indicated. Cryosections were incubated with X-gal (blue staining) and counterstained with Nuclear Fast Red (red staining). Magnification: 40×