Modified univibrator compensates for output timing errors

One-stage, delay compensation amplifier, added to conventional univibrator circuitry time-synchronizes the trailing edge of the output pulse with the origin of the input pulse. The trailing edge is independent of the amplitude of the input pulse

Versatile analog pulse height computer performs real-time arithmetic operations

Multipurpose analog pulse height computer performs real-time arithmetic operations on relatively fast pulses. This computer can be used for identification of charged particles, pulse shape discrimination, division of signals from position sensitive detectors, and other on-line data reduction techniques

Pulse-height defect due to electron interaction in dead layers of Ge/Li/ gamma-ray detectors

Study shows the pulse-height degradation of gamma ray spectra in germanium/lithium detectors to be due to electron interaction in the dead layers that exist in all semiconductor detectors. A pulse shape discrimination technique identifies and eliminates these defective pulses

Mutagenesis of the conserved 51-nucleotide region of Sindbis virus

We have constructed 25 site-specific mutations in a domain of 51 nucleotides in Sindbis virus that is highly conserved among all alphaviruses sequenced to date. These 51 nucleotides are capable of forming two hairpin structures and are found from nucleotides 155 to 205 in Sindbis virus within the region encoding nsP1. Of the mutations, 21 were silent and did not lead to a change in the amino acid sequence encoded. These silent mutations changed not only the linear sequence but also the stability of the hairpins in most cases. Two double mutants that were constructed led to the replacement of one base pair by another so that the linear sequence was altered but the nature of the hairpins was not. All of the mutants with silent mutations were viable, but 19 of the 21 mutants were severely impaired for growth in both chicken and mosquito cells. Compared with the parental virus, they grew slowly and produced virus at rates of 10(-1) to 10(-4) times the parental rate. Surprisingly, however, the plaques produced by these mutants were indistinguishable from those produced by the parental virus. Two of the silent mutations, found within the first hairpin structure, produced virus at a faster rate than the parental virus. It is clear that the exact sequence of this region is important for some aspect of virus replication. We suggest that one or more proteins, either virus encoded or cellular, bind to the hairpin structures in a sequence-specific fashion in a step that promotes replication of the viral RNA. Of the mutations that resulted in a change of coding, only one of four was viable, suggesting that the amino acid sequence encoded in this domain is essential for virus replication

Defined mutations in the 5' nontranslated sequence of Sindbis virus RNA

We have constructed 24 deletion mutants which contain deletions of from 1 to 15 nucleotides in the 5' nontranslated region of Sindbis virus RNA and tested the effect of these mutations on virus replication. The results showed that the first 44 nucleotides, which are capable of forming a hairpin structure, are important for virus replication, as all deletions tested in this region were either lethal or resulted in virus that grew poorly in comparison to the parental virus. Many of these deletions had different effects in mosquito cells than in chicken cells, suggesting that cellular factors, presumably proteins, bind to this region. This domain may function in at least two processes in viral replication. It seems likely that in the minus strand, this sequence element is bound by the viral replicase and promotes RNA replication. In the plus strand, this element may modulate initiation of translation of the nonstructural proteins. The results suggest that the hairpin structure itself is important. All deletions within it had deleterious effects on virus replication, and in particular, deletion of one of the G residues at nucleotide 7 or 8 or of one of the C residues at nucleotide 36 or 37 which are theoretically base-paired with these G's resulted in temperature-sensitive viruses that behaved very similarly. In contrast, large deletions between the 44-nucleotide hairpin and the translation start site at nucleotides 60 to 62 resulted in virus that grew as well as or better than the parental virus in both chicken and mosquito cells. The A residue at position 5 of the HRSP strain used was examined in more detail. Deletion of this A was lethal, whereas substitution by G resulted in a virus that grew poorly, despite the fact that G is present at position 5 in the AR339 parent of HRSP. U at position 5 resulted in a virus that grew less well than the A5 strain but better than the G5 mutant

Sindbis virus ts103 has a mutation in glycoprotein E2 that leads to defective assembly of virions

Sindbis virus mutant ts103 is aberrant in the assembly of virus particles. During virus budding, proper nucleocapsid-glycoprotein interactions fail to occur such that particles containing many nucleocapsids are formed, and the final yield of virus is low. We have determined that a mutation in the external domain of glycoprotein E2, Ala-344-->Val, is the change that leads to this phenotype. Mapping was done by making recombinant viruses between ts103 and a parental strain of the virus, using a full-length cDNA clone of Sindbis virus from which infectious RNA can be transcribed, together with sequence analysis of the region of the genome shown in this way to contain the ts103 lesion. A partial revertant of ts103, called ts103R, was also mapped and sequenced and found to be a second-site revertant in which a change in glycoprotein E1 from lysine to methionine at position 227 partially suppresses the phenotypic effects of the change at E2 position 344. An analysis of revertants from ts103 mutants in which the Ala-->Val change had been transferred into a defined background showed that pseudorevertants were more likely to arise than were true revertants and that the ts103 change itself reverted very infrequently. The assembly defect in ts103 appeared to result from weakened interactions between the virus membrane glycoproteins or between these glycoproteins and the nucleocapsid during budding. Both the E2 mutation leading to the defect in virus assembly and the suppressor mutation in glycoprotein E1 are in the domains external to the lipid bilayer and thus in domains that cannot interact directly with the nucleocapsid. This suggests that in ts103, either the E1-E2 heterodimers or the trimeric spikes (consisting of three E1-E2 heterodimers) are unstable or have an aberrant configuration, and thus do not interact properly with the nucleocapsid, or cannot assembly correctly to form the proper icosahedral array on the surface of the virus

Sindbis virus proteins nsP1 and nsP2 contain homology to nonstructural proteins from several RNA plant viruses

Although the genetic organization of tobacco mosaic virus (TMV) differs considerably from that of the tripartite viruses (alfalfa mosaic virus [AlMV] and brome mosaic virus [BMV]), all of these RNA plant viruses share three domains of homology among their nonstructural proteins. One such domain, common to the AlMV and BMV 2a proteins and the readthrough portion of TMV p183, is also homologous to the readthrough protein nsP4 of Sindbis virus (Haseloff et al., Proc. Natl. Acad. Sci. U.S.A. 81:4358-4362, 1984). Two more domains are conserved among the AlMV and BMV 1a proteins and TMV p126. We show here that these domains have homology with portions of the Sindbis proteins nsP1 and nsP2, respectively. These results strengthen the view that the four viruses share mechanistic similarities in their replication strategies and may be evolutionarily related. These results also suggest that either the AlMV 1a, BMV 1a, and TMV p126 proteins are multifunctional or Sindbis proteins nsP1 and nsP2 function together as subunits in a single complex

Test of a Liquid Argon TPC in a magnetic field and investigation of high temperature superconductors in liquid argon and nitrogen

Tests with cosmic ray muons of a small liquid argon time projection chamber (LAr TPC) in a magnetic field of 0.55 T are described. No effect of the magnetic field on the imaging properties were observed. In view of a future large, magnetized LAr TPC, we investigated the possibility to operate a high temperature superconducting (HTS) solenoid directly in the LAr of the detector. The critical current $I_c$ of HTS cables in an external magnetic field was measured at liquid nitrogen and liquid argon temperatures and a small prototype HTS solenoid was built and tested.Comment: 5 pages, 5 figures, to appear in Proc. of 1st International Workshop towards the Giant Liquid Argon Charge Imaging Experiment (GLA2010), Tsukuba (Japan), March 201

Ultrastable reference pulser for high-resolution spectrometers

Solid-state double-pulse generator for a high resolution semiconductor detector meets specific requirements for resolution /0.05 percent/, amplitude range /0.1-13 MeV/, and repetition rate /0.1-1000 pulses per second/. A tag pulse is generated in coincidence with each reference pulse

Weak Lensing Determination of the Mass in Galaxy Halos

We detect the weak gravitational lensing distortion of 450,000 background galaxies (20<R<23) by 790 foreground galaxies (R<18) selected from the Las Campanas Redshift Survey (LCRS). This is the first detection of weak lensing by field galaxies of known redshift, and as such permits us to reconstruct the shear profile of the typical field galaxy halo in absolute physical units (modulo H_0), and to investigate the dependence of halo mass upon galaxy luminosity. This is also the first galaxy-galaxy lensing study for which the calibration errors are negligible. Within a projected radius of 200 \hkpc, the shear profile is consistent with an isothermal profile with circular velocity 164+-20 km/s for an L* galaxy, consistent with typical disk rotation at this luminosity. This halo mass normalization, combined with the halo profile derived by Fischer et al (2000) from lensing analysis SDSS data, places a lower limit of (2.7+-0.6) x 10^{12}h^{-1} solar masses on the mass of an L* galaxy halo, in good agreement with satellite galaxy studies. Given the known luminosity function of LCRS galaxies, and the assumption that $M\propto L^\beta$ for galaxies, we determine that the mass within 260\hkpc of normal galaxies contributes $\Omega=0.16\pm0.03$ to the density of the Universe (for $\beta=1$) or $\Omega=0.24\pm0.06$ for $\beta=0.5$. These lensing data suggest that $0.6<\beta<2.4$ (95% CL), only marginally in agreement with the usual $\beta\approx0.5$ Faber-Jackson or Tully-Fisher scaling. This is the most complete direct inventory of the matter content of the Universe to date.Comment: 18 pages, incl. 3 figures. Submitted to ApJ 6/7/00, still no response from the referee after four months