Ergebnisse von zytogenetischen Routineuntersuchungen an Bullen in Bayern

International audienc

Pig gene mapping: assignment of the genes for mannosephosphate isomerase (MPI) and nucleoside phosphorylase (NP) to chromosome no. 7

International audienc

C-band variants of telocentric chromosomes in swine: evidence and inheritance studies

International audienc

Provisional assignment of the G blood-group locus to chromosome 15 in swine: Gene mapping in swine using natural and induced marker chromosomes

Using the lod score test, 85 combinations of 19 biochemical and immunogenetic markers and 7 natural and radiation-induced chromosomal markers were checked with respect to linkage. Highly positive lod scores were obtained for the combination of the G blood-group locus with the rob (15;17) centric-fusion chromosome. Positive lod scores also were obtained for the G blood-group locus and the reciprocal translocation rcp (2p+;15q−) marker chromosome. Thus, it was concluded that the locus for the G blood-group system may be on chromosome 15 of swine. For several combinations of markers it was possible to exclude linkage at certain recombination frequencie

Die verwendung zytogenetischer verfahren in der tierzucht

International audienc

Bayesian inference on major loci in related multigeneration selection lines of laying hens

A mixed inheritance model, postulating a polygenic effect and differences between the 3 genotypes of a biallelic locus, was fitted separately to the data of 2 multigeneration selection lines and a control evolving from a common base population. Inferences about the model were drawn from the application of the Gibbs sampler. Body weight at 20 and 40 wk (BW20, BW40) and average egg weight to 40 wk (EW40) were included in the analyses. Significance of differences between posterior means of parameters was established by comparing their 95% highest probability density regions. Significant (P 0.05) differences of posterior means of any parameter could be observed between lines. No significant genotypic or polygenic selection response was found for BW40. On the contrary, both genetic responses were found significant for EW40 in the selected lines, but not in the control. No differences (P > 0.05) between lines could be observed for the derived frequencies of the allele causing the higher trait value and the frequencies of one homozygote and the heterozygote genotypes at the major locus. The detection of a major locus with relatively modest effect confirmed that this type of analysis with data from selected lines was indeed powerfu

Carrier Detection of Ovine Hemophilia A Using an RFLP Marker, and Mapping of the Factor VIII Gene on the Ovine X-Chromosome

Ovine hemophilia A is an X-linked recessive bleeding disorder. For diagnostic purposes, restriction fragment length polymorphism (RFLP) analysis in the region of the factor VIII (F-VIII) gene was carried out using human F-VIII gene probes. The probe St14, Known to detect a highly polymorphic region that is closely linked to the F-VIII gene in humans, hybridized nonspecifcally with DNA from sheep. Searching for Intragenic RFLPs, the entire 9.0-kb coding sequence of the human F-VIII was used as a probe. Using the 1.8-kb Sstl/Kpnl F-VIII cDNA probe for hybridization, an Mspl-RFLP with allelic bands of 5.8 Kb (A1) and 4.2 kb (A2) was detected. A1 was in linkage phase with the mutated allele responsible for hemophilia A. The F-VIII locus in the sheep genome was assigned to the long arm of the X-chromosome in the region Xq24-q33, Using In situ hybridization with a 3-Kb human F-VIII cDNA probe to QFQ banded sheep metaphase chromosome