The Trouble with Jesus

We are turning back to God in great numbers, more than any other modern nation. But attending church and listening to sermons and Scripture can become a superficial exercise. Eventually we realize that we are not satisfied, and that our hearts hunger for a closer walk with Him. When your customers come seeking that greater understanding, be ready with Moody titles that will reveal biblical truths and lead to discussion and understanding.https://digitalcommons.cedarville.edu/alum_books/1180/thumbnail.jp

Radical Reliance

https://digitalcommons.cedarville.edu/alum_books/1255/thumbnail.jp

Intergenerational School Projects: Examples and Guidelines

The residential, educational, and recreational patterns of modern American society tend to isolate the young from the old. Because there is little interaction between the age groups, each generation tends to stereotype the other. Educators must realize that these age-related stereotypes affect our interpersonal relationships, our self-images, and, consequently, our potential to live a full, rewarding life

Delayed Decision-making in Real-time Beatbox Percussion Classification

This is an electronic version of an article published in Journal of New Music Research, 39(3), 203-213, 2010. doi:10.1080/09298215.2010.512979. Journal of New Music Research is available online at: www.tandfonline.com/openurl?genre=article&issn=1744-5027&volume=39&issue=3&spage=20

Bird detection in audio : a survey and a challenge

Many biological monitoring projects rely on acoustic detection of birds. Despite increasingly large datasets, this detection is often manual or semi-automatic, requiring manual tuning/postprocessing. We review the state of the art in automatic bird sound detection, and identify a widespread need for tuning-free and species-agnostic approaches. We introduce new datasets and an IEEE research challenge to address this need, to make possible the development of fully automatic algorithms for bird sound detection

Detection and Classification of Acoustic Scenes and Events

For intelligent systems to make best use of the audio modality, it is important that they can recognize not just speech and music, which have been researched as specific tasks, but also general sounds in everyday environments. To stimulate research in this field we conducted a public research challenge: the IEEE Audio and Acoustic Signal Processing Technical Committee challenge on Detection and Classification of Acoustic Scenes and Events (DCASE). In this paper, we report on the state of the art in automatically classifying audio scenes, and automatically detecting and classifying audio events. We survey prior work as well as the state of the art represented by the submissions to the challenge from various research groups. We also provide detail on the organization of the challenge, so that our experience as challenge hosts may be useful to those organizing challenges in similar domains. We created new audio datasets and baseline systems for the challenge; these, as well as some submitted systems, are publicly available under open licenses, to serve as benchmarks for further research in general-purpose machine listening

Down-regulation of human topoisomerase IIα expression correlates with relative amounts of specificity factors Sp1 and Sp3 bound at proximal and distal promoter regions

<p>Abstract</p> <p>Background</p> <p>Topoisomerase IIα has been shown to be down-regulated in doxorubicin-resistant cell lines. The specificity proteins Sp1 and Sp3 have been implicated in regulation of topoisomerase IIα transcription, although the mechanism by which they regulate expression is not fully understood. Sp1 has been shown to bind specifically to both proximal and distal GC elements of the human topoisomerase IIα promoter <it>in vitro</it>, while Sp3 binds only to the distal GC element unless additional flanking sequences are included. While Sp1 is thought to be an activator of human topoisomerase IIα, the functional significance of Sp3 binding is not known. Therefore, we sought to determine the functional relationship between Sp1 and Sp3 binding to the topoisomerase IIα promoter <it>in vivo</it>. We investigated endogenous levels of Sp1, Sp3 and topoisomerase IIα as well as binding of both Sp1 and Sp3 to the GC boxes of the topoisomerase IIα promoter in breast cancer cell lines <it>in vivo </it>after short term doxorubicin exposure.</p> <p>Results</p> <p>Functional effects of Sp1 and Sp3 were studied using transient cotransfection assays using a topoisomerase IIα promoter reporter construct. The <it>in vivo </it>interactions of Sp1 and Sp3 with the GC elements of the topoisomerase IIα promoter were studied in doxorubicin-treated breast cancer cell lines using chromatin immunoprecipitation assays. Relative amounts of endogenous proteins were measured using immunoblotting. <it>In vivo </it>DNA looping mediated by proteins bound at the GC1 and GC2 elements was studied using the chromatin conformation capture assay. Both Sp1 and Sp3 bound to the GC1 and GC2 regions. Sp1 and Sp3 were transcriptional activators and repressors respectively, with Sp3 repression being dominant over Sp1-mediated activation. The GC1 and GC2 elements are linked <it>in vivo </it>to form a loop, thus bringing distal regulatory elements and their cognate transcription factors into close proximity with the transcription start site.</p> <p>Conclusion</p> <p>These observations provide a mechanistic explanation for the modulation of topoisomerase IIα and concomitant down-regulation that can be mediated by topoisomerase II poisons. Competition between Sp1 and Sp3 for the same cognate DNA would result in activation or repression depending on absolute amounts of each transcription factor in cells treated with doxorubicin.</p