1,446 research outputs found
New genera and species of early Tertiary palynomorphs from Gulf Coast
16 p., 5 pl.http://paleo.ku.edu/contributions.htm
The Efficacy of Commercial Tooth Storage Media for Maintaining the Viability of Human Periodontal Ligament Fibroblasts
Aim
To evaluate SaveâAâTooth (SAT), EMT Toothsaver (EMT) and Hank\u27s Balanced Salt Solution (HBSS) for their influence on the viability and proliferative capacity of human periodontal ligament fibroblasts (HPDLFs). Methodology
Primary HPDLFs were seeded into 96âwell cell culture plates and exposed to SAT, EMT, HBSS and water (negative control) for 0.5, 1, 3, 6, 12 and 24 h at room temperature (22 °C). After each exposure time, cell viability was measured through quantifying adenosine triphosphate (ATP) using a luminescent dye. The proliferative capacity was also quantified using the PrestoBlue assay after 12 or 24 h storage in each medium. The data were analysed statistically by twoâway anova and post hoc Least Significant Difference (LSD) test (P \u3c 0.05). The morphology of the cells after 12 h storage was also investigated through live/dead viability/cytotoxicity kit together with fluorescence microscopy. Results
There was no significant difference in cell viability amongst HBSS, SAT and EMT groups up to 6 h. SAT was effective in maintaining cell viability only up to 12 h and then became detrimental to HPDLF; after 24 h, the effectiveness of SAT in maintaining cell viability was similar to that of water (P \u3e 0.05). Amongst all the media, only EMT could maintain the proliferative capacity of HPDLFs significantly higher than the negative control, that is water (P \u3c 0.05) after 24 h storage. Conclusion
EMT maintained the proliferative capacity of HPDLFs after 24 h storage
Review of SERT 2 power conditioning
SERT 2 spacecraft power conditioner performanc
An atlas of line profile studies for SU UMa type cataclysmic variables
We present H-alpha line-profile analyses for the seven SU UMa type dwarf
novae AK Cnc, WX Cet, AQ Eri, VW Hyi, RZ Leo, TU Men, and HS Vir. All data sets
are treated in the same manner, applying a sequence of techniques for each
system. The basic ingredients of this sequence are the diagnostic diagram to
determine the zero point of the orbital phase, and Doppler tomography to
visualise the emission distribution. We furthermore introduce a new qualitative
way of to evaluate the Doppler fit, by comparing the line profile of the
reconstructed with the original spectrum in the form of the V/R plot. We
present the results of the analysis in the compact form of an atlas, allowing a
direct comparison of the emission distribution in our targets. Although most of
the data sets were not taken with the intention of a line-profile analysis, we
obtain significant results and are able to indicate the type of the additional
emission in these systems. Our objects should have in principle very similar
physical properties, i.e. they cover only a small range in orbital periods,
mass ratios, and mass-transfer rates. Nevertheless, we find a large variety of
phenomena both with respect to the individual systems and also within
individual data sets of the same object. This includes `canonical' additional
emission components from the secondary star and the bright spot, but also
emission from the leading side of the accretion disc.Comment: 20 pages, 25 figures, accepted for publication in A&A, figures have
been diminished in size and qualit
Properdin deficiency protects from 5-fluorouracil-induced small intestinal mucositis in a complement activation-independent, interleukin-10-dependent mechanism
Intestinal mucositis is a serious complication of chemotherapy that leads to significant morbidity that may require dose or drug adjustments. Specific mitigating strategies for mucositis are unavailable, due partly to an incomplete understanding of the pathogenic mechanisms. We have previously shown an effect of properdin, a positive regulator of complement activation, in models of colitis. Here we use properdin-deficient (P) mice to interrogate the role of properdin and complement in small intestinal mucositis. Mucositis was induced by five daily injections of 5-fluorouracil (5-FU) in wild-type (WT), P, interleukin (IL)-10and properdin/IL-10double knock-out (DKO) mice. At the time of euthanasia their jejunum was collected for histology, immunohistochemistry and cytokine and complement activation measurements. Complement became activated in mice receiving 5-FU, indicated by increased intestinal levels of C3a and C5a. Compared to WT, Pmice experienced significantly less mucositis, despite C3a levels as high as inflamed WT mice and slightly less C5a. Conversely, Pmice had higher intestinal levels of IL-10. IL-10 expression was mainly by epithelial cells in both uninflamed and inflamed Pmice. IL-10mice proved to be highly susceptible to mucositis and DKO mice were equally susceptible, demonstrating that a lack of properdin does not protect mice lacking IL-10. We interpret our findings to indicate that, to a significant extent, the inflammation of mucositis is properdin-dependent but complement activation-independent. Additionally, the benefit achieved in the absence of properdin is associated with increased IL-10 levels, and IL-10 is important in limiting mucositis
Star Clusters in the Nearby Late-Type Galaxy NGC 1311
Ultraviolet, optical and near infrared images of the nearby (D ~ 5.5 Mpc) SBm
galaxy NGC 1311, obtained with the Hubble Space Telescope, reveal a small
population of 13 candidate star clusters. We identify candidate star clusters
based on a combination of their luminosity, extent and spectral energy
distribution. The masses of the cluster candidates range from ~1000 up to
~100000 Solar masses, and show a strong positive trend of larger mass with
increasing with cluster age. Such a trend follows from the fading and
dissolution of old, low-mass clusters, and the lack of any young super star
clusters of the sort often formed in strong starbursts. The cluster age
distribution is consistent with a bursting mode of cluster formation, with
active episodes of age ~10 Myr, ~100 Myr and ~1 Gyr. The ranges of age and mass
we probe are consistent with those of the star clusters found in quiescent
Local Group dwarf galaxies.Comment: 21 pages, 11 figures, accepted by A
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