1,351 research outputs found
The Efficacy of Commercial Tooth Storage Media for Maintaining the Viability of Human Periodontal Ligament Fibroblasts
Aim
To evaluate SaveâAâTooth (SAT), EMT Toothsaver (EMT) and Hank\u27s Balanced Salt Solution (HBSS) for their influence on the viability and proliferative capacity of human periodontal ligament fibroblasts (HPDLFs). Methodology
Primary HPDLFs were seeded into 96âwell cell culture plates and exposed to SAT, EMT, HBSS and water (negative control) for 0.5, 1, 3, 6, 12 and 24 h at room temperature (22 °C). After each exposure time, cell viability was measured through quantifying adenosine triphosphate (ATP) using a luminescent dye. The proliferative capacity was also quantified using the PrestoBlue assay after 12 or 24 h storage in each medium. The data were analysed statistically by twoâway anova and post hoc Least Significant Difference (LSD) test (P \u3c 0.05). The morphology of the cells after 12 h storage was also investigated through live/dead viability/cytotoxicity kit together with fluorescence microscopy. Results
There was no significant difference in cell viability amongst HBSS, SAT and EMT groups up to 6 h. SAT was effective in maintaining cell viability only up to 12 h and then became detrimental to HPDLF; after 24 h, the effectiveness of SAT in maintaining cell viability was similar to that of water (P \u3e 0.05). Amongst all the media, only EMT could maintain the proliferative capacity of HPDLFs significantly higher than the negative control, that is water (P \u3c 0.05) after 24 h storage. Conclusion
EMT maintained the proliferative capacity of HPDLFs after 24 h storage
New genera and species of early Tertiary palynomorphs from Gulf Coast
16 p., 5 pl.http://paleo.ku.edu/contributions.htm
Endophytic Agrobacterium in crown gall-resistant and -susceptible Vitis genotypes
Several methods were used to study endophytic colonization of Vitis genotypes by Agrobacterium vitis (AV). AV was seldom detected except at inoculated sites, indicating little systemic movement of the bacterium under the conditions of these experiments. AV populations at inoculated sites were evaluated for 10 months following inoculation of crown gall-resistant and -susceptible genotypes. Two months after inoculation, V. amurensis selections had significantly smaller populations than V. vinifera (Cabernet Sauvignon) or V. riparia x V. rupestris (C3309). All crown gall-resistant genotypes had significantly lower populations than Cabernet Sauvignon 10 months after inoculation. Examination of vines one year after inoculation indicated that AV populations were much higher at inoculated sites when crown galls developed. However, even when no galls were apparent, Cabernet Sauvignon had significantly higher AV populations than V. amurensis 689 (6-fold higher) and C3309 (70-fold higher). Crown gall-resistant genotypes appear to support lower populations of AV than the crow gall-susceptible Cabernet Sauvignon. Freezing followed by a two-day incubation significantly increased recovery of Agrobacterium using vascular fluid displacement in naturally-infected and artifically-inoculated vines and therefore increased the sensitivity of indexing for AV in grapevines
Transformation of crown gall resistant and susceptible Vitis genotypes by Agrobacterium vitis
Transformation of crown gall-susceptible and -resistant Vitis genotypes by Agrobacterium vitis strain CG49 was studied using uidA (GUS) in the p35SGUSINT construct. When greenhouse-grown material propagated through tissue culture was inoculated with CG49(p35SGUSINT) in vitro, the highly crown gall-susceptible V. vinifera Cabernet Sauvignon displayed GUS activity on 53 % of inoculated explants vs. 5 % for the resistant V. amurensis and 0 % for the resistant Couderc 3309. Response of Cabernet Sauvignon suggested a strong effect of shoot polarity on transformation. Inoculation of basal vs. apical explant surface in Cabernet Sauvignon indicated transformation in 88 % of basal inoculated explants with no transformation from apical inoculation. Basal inoculations indicated no transformation of V. amurensis and transformation in 10 % of Couderc 3309 explants. Inoculation of intact plants with CG49(p35SGUSINT) produced GUS-positive sites at 56 % of inoculated sites in Cabernet Sauvignon, 10 % of V. amurensis inoculated sites and 9 % of Couderc 3309 inoculated sites. Resistance to crown gall in these genotypes appears to be due to reduced susceptibility to transformation by A. vitis rather than post-transformation phenomena. These studies were complicated by production of GUS-positive spots from in vitro inoculations using wild-type CG49. Resident microorganisms producing b-glucuronidase may proliferate after tissue degradation by A. vitis-induced cell disruption. Use of in vitro internodal explants from tissue culture-propagated vines greatly reduced GUS expression from control CG49 inoculations and these were readily distinguished (by appearance and location) from GUS-positive spots resulting from transformation with uidA
II-BGlc, a glucose receptor of the bacterial phosphotransferase system: molecular cloning of ptsG and purification of the receptor from an overproducing strain of Escherichia coli.
Asteroseismological Observations of the Central Star of the Planetary Nebula NGC 1501
We report on a global CCD time-series photometric campaign to decode the
pulsations of the nucleus of the planetary nebula NGC1501. The star is hot and
hydrogen-deficient, similar to the pre-white-dwarf PG 1159 stars. NGC1501 shows
pulsational brightness variations of a few percent with periods ranging from 19
to 87 minutes. The variations are very complex, suggesting a pulsation spectrum
that requires a long unbroken time series to resolve. Our CCD photometry of the
star covers a two-week period in 1991 November, and used a global network of
observatories. We obtained nearly continuous coverage over an interval of one
week in the middle of the run. We have identified 10 pulsation periods, ranging
from 5235 s down to 1154 s. We find strong evidence that the modes are indeed
nonradial g-modes. The ratios of the frequencies of the largest-amplitude modes
agree with those expected for modes that are trapped by a density discontinuity
in the outer layers. We offer a model for the pulsation spectrum that includes
a common period spacing of 22.3 s and a rotation period of 1.17 days; the
period spacing allows us to assign a seismological mass of 0.55+/-0.03 Msun.Comment: 12 pages, AASTEX, 7 tables, 6 EPS figures, to appear in AJ, 12/96
Corrected version repairs table formatting and adds missing Table
An atlas of line profile studies for SU UMa type cataclysmic variables
We present H-alpha line-profile analyses for the seven SU UMa type dwarf
novae AK Cnc, WX Cet, AQ Eri, VW Hyi, RZ Leo, TU Men, and HS Vir. All data sets
are treated in the same manner, applying a sequence of techniques for each
system. The basic ingredients of this sequence are the diagnostic diagram to
determine the zero point of the orbital phase, and Doppler tomography to
visualise the emission distribution. We furthermore introduce a new qualitative
way of to evaluate the Doppler fit, by comparing the line profile of the
reconstructed with the original spectrum in the form of the V/R plot. We
present the results of the analysis in the compact form of an atlas, allowing a
direct comparison of the emission distribution in our targets. Although most of
the data sets were not taken with the intention of a line-profile analysis, we
obtain significant results and are able to indicate the type of the additional
emission in these systems. Our objects should have in principle very similar
physical properties, i.e. they cover only a small range in orbital periods,
mass ratios, and mass-transfer rates. Nevertheless, we find a large variety of
phenomena both with respect to the individual systems and also within
individual data sets of the same object. This includes `canonical' additional
emission components from the secondary star and the bright spot, but also
emission from the leading side of the accretion disc.Comment: 20 pages, 25 figures, accepted for publication in A&A, figures have
been diminished in size and qualit
Star Clusters in the Nearby Late-Type Galaxy NGC 1311
Ultraviolet, optical and near infrared images of the nearby (D ~ 5.5 Mpc) SBm
galaxy NGC 1311, obtained with the Hubble Space Telescope, reveal a small
population of 13 candidate star clusters. We identify candidate star clusters
based on a combination of their luminosity, extent and spectral energy
distribution. The masses of the cluster candidates range from ~1000 up to
~100000 Solar masses, and show a strong positive trend of larger mass with
increasing with cluster age. Such a trend follows from the fading and
dissolution of old, low-mass clusters, and the lack of any young super star
clusters of the sort often formed in strong starbursts. The cluster age
distribution is consistent with a bursting mode of cluster formation, with
active episodes of age ~10 Myr, ~100 Myr and ~1 Gyr. The ranges of age and mass
we probe are consistent with those of the star clusters found in quiescent
Local Group dwarf galaxies.Comment: 21 pages, 11 figures, accepted by A
An evaluation of metal removal during wastewater treatment: The potential to achieve more stringent final effluent standards
This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2011 Taylor & Francis.Metals are of particular importance in relation to water quality, and concern regarding the impact of these contaminants on biodiversity is being encapsulated within the latest water-related legislation such as the Water Framework Directive in Europe and criteria revisions to the Clean Water Act in the United States. This review undertakes an evaluation of the potential of 2-stage wastewater treatment consisting of primary sedimentation and biological treatment in the form of activated sludge processes, to meet more stringent discharge consents that are likely to be introduced as a consequence. The legislation, sources of metals, and mechanisms responsible for their removal are discussed, to elucidate possible pathways by which the performance of conventional processes may be optimized or enhanced. Improvements in effluent quality, achievable by reducing concentrations of suspended solids or biochemical oxygen demand, may also reduce metal concentrations although meeting possible requirements for the removal of copper my be challenging
Irradiation of the secondary star in X-ray Nova Scorpii 1994 (=GRO J1655--40)
We have obtained intermediate resolution optical spectra of the black-hole
candidate Nova Sco 1994 in June 1996, when the source was in an X-ray/optical
active state (R~15.05). We measure the radial velocity curve of the secondary
star and obtain a semi-amplitude of 279+/-10 km/s; a value which is 30 per cent
larger than the value obtained when the source is in quiescence. Our large
value for K_2 is consistent with 60 +9,-7 per cent of the secondary star's
surface being heated; compared to 35 per cent, which is what one would expect
if only the inner face of the secondary star were irradiated. Effects such as
irradiation-induced flows on the secondary star may be important in explaining
the observed large value for K_2.Comment: 5 pages, 2 figures, accepted by MNRA
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