Prenatal inhibition of the tryptophan–kynurenine pathway alters synaptic plasticity and protein expression in the rat hippocampus

Glutamate receptors sensitive to N-methyl-d-aspartate (NMDA) are important in early brain development, influencing cell proliferation and migration, neuritogenesis, axon guidance and synapse formation. The kynurenine pathway of tryptophan metabolism includes an agonist (quinolinic acid) and an antagonist (kynurenic acid) at these receptors. Rats were treated in late gestation with 3,4-dimethoxy-N-[4-(3-nitrophenyl)thiazol-2-yl]-benzene-sulphonamide (Ro61-8048), an inhibitor of kynurenine-3-monoxygenase which diverts kynurenine metabolism to kynurenic acid. Within 5 h of drug administration, there was a significant decrease in GluN2A expression and increased GluN2B in the embryo brains, with changes in sonic hedgehog at 24 h. When injected dams were allowed to litter normally, the brains of offspring were removed at postnatal day 21 (P21). Recordings of hippocampal field excitatory synaptic potentials (fEPSPs) showed that prenatal exposure to Ro61-8048 increased neuronal excitability and paired-pulse facilitation. Long-term potentiation was also increased, with no change in long-term depression. At this time, levels of GluN2A, GluN2B and postsynaptic density protein PSD-95 were all increased. Among several neurodevelopmental proteins, the expression of sonic hedgehog was increased, but DISC1 and dependence receptors were unaffected, while raised levels of doublecortin and Proliferating Cell Nuclear Antigen (PCNA) suggested increased neurogenesis. The results reveal that inhibiting the kynurenine pathway in utero leads to molecular and functional synaptic changes in the embryos and offspring, indicating that the pathway is active during gestation and plays a significant role in the normal early development of the embryonic and neonatal nervous system

Techniques for the realization of ultrareliable spaceborne computers Interim scientific report

Error-free ultrareliable spaceborne computer

Field Dependent Phase Diagram of the Quantum Spin Chain (CH3)2NH2CuCl3

Although (CH3)2NH2CuCl3 (MCCL) was first examined in the 1930's [1], there are open questions regarding the magnetic dimensionality and nature of the magnetic properties. MCCL is proposed to be a S=1/2 alternating ferromagnetic antiferromagnetic spin chain alternating along the crystalline a-axis [2,3]. Proposed ferromagnetic (JFM =1.3 meV) and antiferromagnetic (JAFM =1.1 meV) exchange constants make this system particularly interesting for experimental study. Because JFM and JAFM are nearly identical, the system should show competing behavior between S=1/2 (AFM) and S=1(FM) effects. We report low temperature magnetic field dependent susceptibility, chi(H), and specific heat, Cp, of MCCL. These provide an initial magnetic-field versus temperature phase diagram. A zero-field phase transition consistent with long range magnetic order is observed at T=0.9 K. The transition temperature can be reduced via application of a magnetic field. We also present comparisons to a FM/AFM dimer model that accounts for chi(T,H=0) and Cp(H,T).Comment: 2 pages, 1 figure included in text. Submitted to proceedings of 24th International Conference on Low Temperature Physics, August 200

The Energy Spectrum of Jovian Electrons in Interplanetary Space

The energy spectrum of electrons with energies approx 10 to approx 180 MeV measured with the electron telescope on the Voyager 1 and 2 spacecraft in interplanetary space from 1978 to 1983 is studied. The kinetic energy of electrons is determined by double dE/dx measurements from the first two detectors (D sub 1, D sub 2) of a stack of eight solid state detectors and by the range of particle penetration into the remaining six detectors (D sub 3 to D sub 8) which are interleaved with tungsten absorbers. From 1978 to 1983 (radial range approximately 2 to a pproximately 12 AU) electrons of Jovian origin were clearly observable for electrons stopping in D(sub 3(E approximately greater than 4 MeV)) and in D(sub 4 (E approximately greater than 8 MeV)). For electrons stopping in D(sub 5(E approximately greather than 12 MeV)), the jovian flux dominated the galactic electron flux for a period of approximately one year near the encounter with Jupiter. Jovian electrons were also observed in D(sub 6(E approximately greater than 21 MeV)) but not in D(sub 7(E approximately greater than 28 MeV)). A detailed interpretation of the electron variations in all energy channels depends on an accurate subtraction of background induced by energetic protons of a few 100 MeV. This substraction is facilitated by laboratory calibration results at several energies. Further results on the differential energy spectrum of Jovian electrons and limits on the maximum detected energies will be reported

Two-Particle Microrheology of quasi-2D Viscous Systems

We study the correlated motions of colloidal particles in a quasi-2D system (Human Serum Albumin (HSA) protein molecules at an air-water interface) for different surface viscosities $\eta_{s}$. We observe a transition in the behavior of the correlated motion, from 2-D interface dominated at high $\eta_{s}$ to bulk fluid-dependent at low $\eta_{s}$. The correlated motions can be scaled onto a master curve which captures the features of this transition. This master curve also characterizes the spatial dependence of the flow field of a viscous interface in response to a force. From the flow field and the correlated particle motions, we calculate a two-particle MSD (mean square displacement) for direct comparison with rheological measurements.Comment: 4 pages, 4 figures, submitted to PR

Classical limit of transport in quantum kicked maps

We investigate the behavior of weak localization, conductance fluctuations, and shot noise of a chaotic scatterer in the semiclassical limit. Time resolved numerical results, obtained by truncating the time-evolution of a kicked quantum map after a certain number of iterations, are compared to semiclassical theory. Considering how the appearance of quantum effects is delayed as a function of the Ehrenfest time gives a new method to compare theory and numerical simulations. We find that both weak localization and shot noise agree with semiclassical theory, which predicts exponential suppression with increasing Ehrenfest time. However, conductance fluctuations exhibit different behavior, with only a slight dependence on the Ehrenfest time.Comment: 17 pages, 13 figures. Final versio

Effects of a Local Interstellar Magnetic Field on Voyager 1 and 2 Observations

We show that that an interstellar magnetic field can produce a north/south asymmetry in solar wind termination shock. Using Voyager 1 and 2 measurements, we suggest that the angle $\alpha$ between the interstellar wind velocity and magnetic field is $30^{\circ} < \alpha < 60^{\circ}$. The distortion of the shock is such that termination shock particles could stream outward along the spiral interplanetary magnetic field connecting Voyager 1 to the shock when the spacecraft was within $\sim 2~AU$ of the shock. The shock distortion is larger in the southern hemisphere, and Voyager 2 could be connected to the shock when it is within $\sim 5~AU$ of the shock, but with particles from the shock streaming inward along the field. Tighter constraints on the interstellar magnetic field should be possible when Voyager 2 crosses the shock in the next several years.Comment: 12 pages, 5 figure