4 research outputs found
Upravljanje resursima u HPLC: Otkrivanje zelenog lica farmaceutske analize
High-pressure liquid chromatography (HPLC) is a technique of paramount importance in
the analysis of pharmaceuticals because of its ability to separate moderately polar to less polar
compounds, such as drugs and related substances. The concept of green analytical chemistry
(GAC) aims to provide more environmentally friendly and safer analytical methods in terms of
reagents, energy, and waste. One of the major challenges of GAC is to find an appropriate
approach to evaluate the greenness of analytical methods. An extension of GAC, called white
analytical chemistry (WAC), has been introduced to consider not only environmental friendliness,
but also other aspects that contribute to the sustainability of methods, such as analytical and
economic or practical efficiency. HPLC methods are intrinsically not green, due to the high
consumption of toxic organic solvents and the resulting generation of large amounts of toxic
waste. Fortunately, there are many approaches to overcome the non-green character of HPLC
methods. In this article, various modifications of the HPLC methods that increase its
environmental friendliness are presented, as well as the various tools used to evaluate
environmental friendliness. In addition, the new concept of white analytical chemistry is
presented.TeÄna hromatografija pod visokim pritiskom (HPLC) je tehnika od ogromne važnosti u analitici lekova zbog svoje moguÄnosti da razdvoji umereno do manje polarna jedinjenja, kao Å”to su aktivne farmaceutske i srodne supstance. Koncept zelene analitiÄke hemije (GAC) ima za cilj da obezbedi ekoloÅ”ki prihvatljivije i bezbednije metode u pogledu reagenasa, energije i otpada. Jedan od glavnih izazova GAC je da pronaÄe odgovarajuÄi pristup za procenu "zelenosti" analitiÄkih metoda. ProÅ”irenje GAC, nazvano bela analitiÄka hemija (WAC), uvedeno je kako bi se uzela u obzir ne samo ekoloÅ”ka prihvatljivost, veÄ i drugi aspekti koji doprinose održivosti metoda, kao Å”to su analitiÄka i ekonomska ili praktiÄna efikasnost. HPLC metode suÅ”tinski nisu "zelene" zbog obimne potroÅ”nje toksiÄnih organskih rastvaraÄa i poslediÄnog stvaranja velikih koliÄina toksiÄnog otpada. SreÄom, postoji mnogo pristupa za prevazilaženje ne-zelene prirode HPLC metoda. U ovom radu su predstavljene razliÄite modifikacije HPLC metode koje poveÄavaju ekoloÅ”ku prihvatljivost, kao i razliÄiti alati koji se koriste za procenu ekoloÅ”ke prihvatljivosti. Pored toga, predstavljen je novi koncept bele analitiÄke hemije
Revealing Retention Mechanisms in Liquid Chromatography: QSRR Approach
One-factor-at-a-time experimentation was used for a long time as gold-standard optimization for liquid chromatographic (LC) method development. This approach has two downsides as it requires a needlessly great number of experimental runs and it is unable to identify possible factor interactions. At the end of the last century, however, this problem could be solved with the introduction of new chemometric strategies. This chapter aims at presenting quantitative structureāretention relationship (QSRR) models with structuring possibilities, from the point of feature selection through various machine learning algorithms that can be used in model building, for internal and external validation of the proposed models. The presented strategies of QSRR model can be a good starting point for analysts to use and adopt them as a good practice for their applications. QSRR models can be used in predicting the retention behavior of compounds, to point out the molecular features governing the retention, and consequently to gain insight into the retention mechanisms. In terms of these applications, special attention was drawn to modified chromatographic systems, characterized by mobile or stationary phase modifications. Although chromatographic methods are applied in a wide variety of fields, the greatest attention has been devoted to the analysis of pharmaceuticals
Primena eksperimentalnog dizajna za razdvajanje lekova HPLC metodom
Design of Experiments (DoE) is an indispensable tool in contemporary drug analysis as it
simultaneously balances a number of chromatographic parameters to ensure optimal separation
in High Pressure Liquid Chromatography (HPLC). This manuscript briefly outlines the theoretical
background of the DoE and provides step-by-step instruction for its implementation in HPLC
pharmaceutical practice. It particularly discusses the classification of various design types and
their possibilities to rationalize the different stages of HPLC method development workflow, such
as the selection of the most influential factors, factors optimization and assessment of the method
robustness. Additionally, the application of the DoE-based Analytical Quality by Design (AQbD)
concept in the LC method development has been summarized. Recent achievements in the use of
DoE in the development of stability-indicating LC and hyphenated LC-MS methods have also
been briefly reported. Performing of Quantitative structure retention relationship (QSRR) study
enhanced with DoE-based data collection was recomended as a future perspective in description
of retention in HPLC system.Dizajn eksperimenata (DoE) je nezaobilazan alat u savremenoj analizi lekova buduÄi da istovremeno balansira niz hromatografskih parametara kako bi se osiguralo optimalno razdvajanje u teÄnoj hromatografiji pod visokim pritiskom (HPLC). Prikazana je teorijska osnova DOE i data su detaljna uputstva za njegovu primenu u HPLC ispitivanjima u farmaciji. NaroÄito se govori o klasifikaciji brojnih tipova dizajna i njihovim moguÄnostima za racionalizaciju razliÄitih faza tokom procesa razvoja HPLC metode, kao Å”to su izbor najuticajnijih faktora, optimizacija faktora i procena robusnosti metode. Dodatno, sumirana je primena DOE kao sastavnog dela koncepta ugradnje kvaliteta u proizvod u domenu razvoja analitiÄkih metoda (AQbD) zasnovanih na HPLC tehnici. TakoÄe su prikazana i nedavna dostignuÄa u primeni DOE u razvoju LC metoda koje su pogodne za ispitivanje stabilnosti lekova, kao i LC-MS metoda. U buduÄoj perspektivi, preporuÄeno je izvoÄenje ispitivanja kvantitativnog odnosa izmeÄu strukture i retencionog ponaÅ”anja (QSRR) analita u HPLC sistemu na osnovu podataka dobijenih primenom DOE
266 Koncept ekoloÅ”ki prihvatljivih hromatografskih metoda: Studija sluÄaja na primeru razdvajanja dronedaron hidrohlorida i njegovih degradacionih proizvoda
Recently, concern about the environmental impact of drug analysis methods has increased
significantly. Reversed-phase high-performance liquid chromatography (RP-HPLC), the
predominant technique in drug analysis, relies heavily on organic solvents such as acetonitrile,
which is known for its chromatographic efficiency, but also for its toxicity and flammability. To
address these concerns, it is essential to minimize the use of toxic organic solvents. The aim of
this study is to explore greener RP-HPLC modifications and evaluate their applicability in the
pharmaceutical industry. Methods were developed for the separation of dronedarone
hydrochloride and its degradation products based on experimental design, including micellar
liquid chromatography (MLC), Ī²-cyclodextrin (CD) modified RP-HPLC and ultra-high
performance liquid chromatography (UHPLC). The eco-friendliness of these methods was
assessed using the analytical eco-scale score, green analytical procedure index (GAPI) and
analytical greenness (AGREE). AGREE appears to be the most suitable, as it revealed the greatest
differences between the compared methods, as well as insights into critical aspects of the methods.
UHPLC and Ī²-CD modified RP-HPLC have been shown to be superior to MLC, and both
methods can be a good choice, depending on whether the ease of implementation or energy
efficiency is considered to be a more important criterion.U poslednje vreme, zabrinutost za negativan uticaj metoda koje se koriste u analitici lekova
na životnu sredinu je u znaÄajnom porastu. Reverzno-fazna teÄna hromatografija visokih
performansi (RP-HPLC) kao dominantno koriÅ”Äena tehnika u velikoj meri se oslanja na primenu
organskih rastvaraÄa, poput acetonitrila, koji je poznat po hromatografskoj efikasnosti, ali i po
toksiÄnosti i zapaljivosti. Kako bi se ovi problemi reÅ”ili i zaÅ”titilo zdravlje ljudi i životna sredina,
neophodno je upotrebu toksiÄnih organskih rastvaraÄa svesti na minimum. Cilj ovog istraživanja
bio je da preporuÄi āzelenijeā modifikacije RP-HPLC metoda. Primenom eksperimentalnog
dizajna razvijene su metode za razdvajanje dronedaron-hidrohlorida i njegovih degradacionih
proizvoda, ukljuÄujuÄi micelarnu teÄnu hromatografiju (MLC), RP-HPLC metodu modifikovanu
Ī²-ciklodekstrinom (CD) i teÄnu hromatografiju ultra visokih performansi (UHPLC). EkoloÅ”ka
prihvatljivost ovih metoda je procenjena koriÅ”Äenjem analitiÄke eko-skale, indeksa zelene
analitiÄke procedure (GAPI) i pristupa analitiÄke zelenosti (AGREE). AGREE se izdvojio kao
najpogodniji, jer je pokazao najveÄe razlike izmeÄu navedenih metoda, kao i uvid u kritiÄne
aspekte metoda. UHPLC i Ī²-CD modifikovana RP-HPLC metoda su se pokazale superiornim u
odnosu na MLC. Koja metoda Äe biti metoda izbora zavisi od toga da li se lakoÄa implementacije
ili energetska efikasnost smatraju važnijim kriterijumom