Chapter L’applicazione di tecniche innovative nel monitoraggio costiero degli habitat prioritari

The aim of the present paper is to define the advantage to use innovative techniques based on sperimental tool to supplement the traditional techniques in marine monitoring, through experience of CRSM-ARPACAL (Centro Regionale Strategia Marina) into two regional projects called "SIC Carlit" and "Musmap". Both projects have shown that in the monitoring of coastal marine ecosystems the sperimental techniques to supplement traditional methods can provide more accurate and data with reduced costs and times of work

A new proof of evidence of cysteamine quantification for therapeutic drug monitoring in patients with cystinosis

Background To date, measurement of intracellular cystine is used for the therapeutic monitoring of patients affected by cystinosis in treatment with cysteamine. Since this method is time and sample consuming, development of a faster method to quantify cysteamine would be extremely useful in order to help clinicians to adjust dosages of cysteamine and to define better the pharmacokinetic profile of this drug. The aim of the study was to develop a liquid chromatography tandem mass spectrometry method for the quantification of cysteamine in plasma samples and to test its applicability on plasma samples derived from patients with nephropathic infantile cystinosis in treatment with cysteamine. Results The percentage of accuracy of the developed method varied between 97.80 and 106.00% and CV% between 0.90 and 6.93%. There was no carry over. The calibration curves were built from 2.5 to 50 mu M. The limit of detection and the lower limit of quantification occurred at 0.25 and 1.25 mu M respectively. Cysteamine was stable up to 2 months at -20 degrees C. Concentrations of cysteamine and intracellular cystine of 4 patients were in line with data previously reported. Conclusion The proposed method showed an appropriate selectivity, specificity, linearity, sensibility, accuracy, precision and good applicability to samples

Chapter L’applicazione di tecniche innovative nel monitoraggio costiero degli habitat prioritari

The aim of the present paper is to define the advantage to use innovative techniques based on sperimental tool to supplement the traditional techniques in marine monitoring, through experience of CRSM-ARPACAL (Centro Regionale Strategia Marina) into two regional projects called "SIC Carlit" and "Musmap". Both projects have shown that in the monitoring of coastal marine ecosystems the sperimental techniques to supplement traditional methods can provide more accurate and data with reduced costs and times of work

Quantification of 7 cannabinoids in cannabis oil using GC-MS: Method development, validation and application to therapeutic preparations in Friuli Venezia Giulia region, Italy

The use of therapeutic cannabis preparations in Friuli Venezia Giulia is increasingly expanding. Even if cannabis oil finds its applications in several disorders affecting adults and children, it is not yet a standardized product and, to ensure the quality of the preparation, a quantitative analysis must be carried out before dispensing it to patients. Gas chromatography coupled to mass spectrometry (GC-MS) is a frequently used technique for quantification of cannabinoids, the active compounds of C. sativa. In this context, we developed a GC-MS method for the simultaneous quantification of 7 cannabinoids (CBD, CBDA, CBG, CBN, THCA, THCV and Δ9-THC) that is not time and sample consuming: 10 μL of cannabis oil were used for the sample preparation, that consists in derivatization of analytes through silylation. Calibration curves were built from 0.2 to 2 μg/mL. The percentage of accuracy and precision did not exceed the values recommended by validation guidelines. The limit of detection was 0.01 μg/mL; whereas the lower limit of quantification was 0.2 μg/mL. There was no carry over. The proposed GC-MS method showed good sensitivity, specificity, linearity, accuracy, precision and applicability to therapeutic preparations