Evidence of bacteriogenic iron and manganese oxyhydroxides in Albian-Cenomanian marine sediments of the Carpathian realm (Poland)

The Albian and Cenomanian marine sediments of the Silesian and Tatric basins in the Carpathian realm of the Western Tethys contain ferric and ferromanganese oxyhydroxides, visible macroscopically as brown stainings. They coat calcareous bioclasts and mineral clasts, fill pore spaces, or locally form continuous, parallel microlayers, tens of micrometers thick. Light-microscope (LM) and scanning-electron-microscope (SEM) obser- vations show that the coatings contain elongated capsules, approximately 3-5 \mum across and enriched in iron and manganese, which may be remnants of the original sheaths of iron-related bacteria (IRB). Moreover, the ferric and ferromanganese staining observed under LM is similar to bacterial structures, resembling the sheaths, filaments and rods formed by present-day bacteria of the Sphaerotilus-Leptothrix group. All of the possible bacteria-like structures are well preserved owing to processes of early diagenetic cementation. If the observed structures are fossil IRB, these organisms could have played an important role in iron and manganese accumulation on the sea floor during Albian-Cenomanian time. The most plausible source of metals for bacterial concentration in the Silesian Basin might have been submarine low-temperature hydrothermal vents, as previously was hypothesized for Cenomanian-Turonian deposits on the basis of geochemical indices

Successive stages of calcitization and silicification of Cenomanian spicule-bearing turbidites based on microfacies analysis, Polish Outer Carpathians

Mid-Cretaceous turbidites with large proportions of sponge spicules are widely distributed in the Silesian Nappe of the Outer Carpathians, giving rise to diversified types of sediments, from spiculites to spicule-bearing siliciclastics and calcarenites. Part of this succession, Middle–Late Cenomanian in age, was transformed into cherts. A microfacies study showed that these turbidite sediments underwent several stages of calcitization and silicification, which took place during Mid-Cretaceous times in different sedimentary environments, i.e., on a northern shelf bordering the Silesian Basin and on a deep sea floor. The first diagenetic changes were related to changes to the biotic components of the turbidite layers, dominated by siliceous sponge spicules. This process, which took place in the spiculitic carbonate mud on the shelves, was related to the calcitization of sponge spicules. Calcareous clasts and calcified skeletal elements also were corroded by bacteria. After transportation down the slope, the biogenic and siliciclastic particles were deposited below the carbonate compensation depth. Taphonomic processes on the basin floor and alternating phases of carbonate and silica cementations, recrystallization and dissolution occurred in these sediments and were related to the diversification in composition of successive turbidite layers. Silicification was related to the formation of quartz precipitates as fibrous chalcedony or microcrystalline quartz, which were derived from the earlier dissolution of amorphous silica, originating mostly from siliceous sponge spicules and radiolarian skeletons. However, a source of silica from hydrothermal vents was also possible. The initial silica precipitation could have taken place in a slightly acidic environment, where calcite was simultaneously dissolved. A number of silicification stages, visible as different forms of silica precipitate inside moulds after bioclasts, occur in the particular turbidite layers. They were related to changes in various elements of the pore-water profile after descending turbidity-current flows. A very low sedimentation rate during the Middle–Late Cenomanian in the Silesian Basin may have favoured the sequence of initial calcitization and silicification stages of the turbidite sediments

Bacteriogenic iron oxides as an evidence of hydrothermal vent activity : case study from the Polish part of the Silesian Nappe and Tatra Massif

Ferruginous (i.e. ferrous oxides/hydroxides) coatings occurring in the Upper Albian– Cenomanian marine sediments of the Carpathians, represent the occurrence of Iron Related Bacteria. The singular grain coatings consist of densely packed elongated capsules of 5 μm width, with a wall thickness of 0.1 μm. These structures are remnants of the original bacterial sheath. The chemical composition of the capsules and their morphology show that they may be representatives of the Sphaerotilus–Leptothrix group, living recently around low-temperature hydrothermal vents. Such vents are interpreted as the source of metals for bottom sediments in the Silesian Basin (Outer Carpathians) and most probably in the Tatric Basin (Inner Carpathians), too

Response to treatment with recombinant human growth hormone (rhGH) of short stature children born too small for gestational age (SGA) in selected centres in Poland

Short stature resulting from SGA is an obligatory indication for treatment with rhGH. The aim of the study was to assess the response to rhGH treatment in patients treated in the years 2016–2020 in six clinical centers in Poland. During the analysis, auxological data were collected, and anthropometrical parameters (Ht, SDS Ht, HV and ΔHV) were reassessed. Subgroups of patients with dysmorphic features (DYSM), fetal alcohol syndrome (FAS) and Silver-Russel syndrome (SRS) were selected. The study group consisted of 235 children (137 boys). The medium initial age was 9.08 years, and 190 patients were in the prepubertal stage. The poor response to treatment was defined as ΔHt SDS < 0.3 and/or ΔHV < 3 cm/year. Seventeen per cent of all patients after the first year and 44% after the second year met the ΔHt SDS < 0.3 criterion, and 56% during the first and 73% during the second year met the ΔHV < 3 cm/year criterion. Our data suggest that patients with SRS may show the best response to treatment, which was sustained throughout the follow-up period. The best response in all subgroups was observed during the first 12 months of therapy. Although the proportion of patients meeting the poor response criteria was high, only a few patients exceeded the 97th percentile for IGF-1 concentration during the first year of treatment. This might suggest that increasing the dose of rhGH in the second treatment year in order to sustain accelerated HV would be safe in these patients

Functional TSH receptor antibodies in children with autoimmune thyroid diseases

Introduction: The diagnostic value of the level of TSH receptor antibodies (TSHR-Ab) in the population of children with autoimmune thyroid diseases (AITDs) is still unknown. The aim of this cross-sectional study was to investigate the prevalence of TSHR-Ab in a paediatric cohort with AITD and healthy controls. Materials and methods: A total of 240 serum samples were obtained from 205 patients with AITD, type 1 diabetes (T1D), juvenile arthritis (JA), and healthy controls (C). TSHR stimulating (TSI) and -blocking (TBI) immunoglobulins were measured in cell-based bioassays using CHO cells expressing a chimeric TSHR and a c-AMP response-element-dependent luciferase. TSI was reported as percentage of specimen-to-reference ratio (cutoff 140SRR%). Blocking activity was defined as percent inhibition of luciferase expression relative to induction with bovine TSH alone (40% inhibition). Results: C as well as children with JA and T1D were both TSI and TBI negative. In contrast, children with Graves’ disease (GD) were positive for TSI in 47/53 samples (88.7%) while those with thyroidal and orbital GD showed TSI positivity in 95.8% (23/24 samples). Serum TSI levels were SRR% 320 ± 157 and 417 ± 135 in GD and GD + orbitopathy, respectively (p = .02). Children with Hashimoto’s thyroiditis (HT) were TSI positive in 4/83 (4.8%) samples, including two with orbital involvement. TSI levels were increased in HT children with vs. those without eye disease (SRR% 177 vs. 51, p < .01). In comparison, TBI were negative in all tested samples of children with GD but positive in one HT sample. Conclusions: In conclusion, TSI is prevalent in children with GD while the highest serum TSI levels were noted in children with AITD and orbitopathy