Spatiotemporal transcriptomic maps of whole mouse embryos at the onset of organogenesis

Spatiotemporal orchestration of gene expression is required for proper embryonic development. The use of single-cell technologies has begun to provide improved resolution of early regulatory dynamics, including detailed molecular definitions of most cell states during mouse embryogenesis. Here we used Slide-seq to build spatial transcriptomic maps of complete embryonic day (E) 8.5 and E9.0, and partial E9.5 embryos. To support their utility, we developed sc3D, a tool for reconstructing and exploring three-dimensional ‘virtual embryos’, which enables the quantitative investigation of regionalized gene expression patterns. Our measurements along the main embryonic axes of the developing neural tube revealed several previously unannotated genes with distinct spatial patterns. We also characterized the conflicting transcriptional identity of ‘ectopic’ neural tubes that emerge in Tbx6 mutant embryos. Taken together, we present an experimental and computational framework for the spatiotemporal investigation of whole embryonic structures and mutant phenotypes

The 2017 French Presidential Election: Students\u27 Mock Party Platforms, Logos, and Campaign Ads (session conducted in French)

Students enrolled in FRN 221 analyze the build-up to the French presidential election scheduled on April 23 and May 7 2017. Grounding their work in the knowledge they have acquired thus far this semester (institutions, political history, current issues, etc.), they have created their own political parties, platforms, campaign ads and logos. In this session, students present their mock political parties. They will highlight the current French issues they are trying to address in their platform, present their party logo, and screen their campaign ads