Comparison of the <i>Vibrio</i> counts obtained using both the TCBS plate culturing and ELISA detection method.

<p>Comparison of the <i>Vibrio</i> counts obtained using both the TCBS plate culturing and ELISA detection method.</p

Affinity of the different <i>Vibrio</i> strains and commercial positive control to the functionalised surface using optimised conditions.

<p>Absorbance signals obtained after a 30 min cell capture step on the functionalised surface and a 30 min detection step using a 1/1000 dilution of horseradish peroxidase anti-<i>Vibrio</i> antibody (HRP-α<i>Vib</i> Pab).</p

LOD, IC50 and sensitivity values obtained for each <i>Vibrio</i> strain with or without functionalising the plate surface.

<p>LOD, IC50 and sensitivity values obtained for each <i>Vibrio</i> strain with or without functionalising the plate surface.</p

Effect of the capture medium.

<p>Signals obtained for increasing <i>Vibrio parahaemolyticus</i> (dashed line) or <i>S. marcescens</i> (solid line) cell concentrations when carrying out the capture step either in PBS (▪), filtered (▴) or unfiltered (•) natural seawater.</p

Surface Immuno-Functionalisation for the Capture and Detection of <i>Vibrio</i> Species in the Marine Environment: A New Management Tool for Industrial Facilities

<div><p>Bacteria from the genus <i>Vibrio</i> are a common and environmentally important group of bacteria within coastal environments and include species pathogenic to aquaculture organisms. Their distribution and abundance are linked to specific environmental parameters, including temperature, salinity and nutrient enrichment. Accurate and efficient detection of Vibrios in environmental samples provides a potential important indicator of overall ecosystem health while also allowing rapid management responses for species pathogenic to humans or species implicated in disease of economically important aquacultured fish and invertebrates. In this study, we developed a surface immuno-functionalisation protocol, based on an avidin-biotin type covalent binding strategy, allowing specific sandwich-type detection of bacteria from the <i>Vibrio</i> genus. The assay was optimized on 12 diverse <i>Vibrio</i> strains, including species that have implications for aquaculture industries, reaching detection limits between 7×10³ to 3×10⁴ cells mL⁻¹. Current techniques for the detection of total Vibrios rely on laborious or inefficient analyses resulting in delayed management decisions. This work represents a novel approach for a rapid, accurate, sensitive and robust tool for quantifying Vibrios directly in industrial systems and in the environment, thereby facilitating rapid management responses.</p></div

Linear part of the ELISA standard curve.

<p>Absorbance values obtained for increasing <i>V. parahaemolyticus</i> cell concentrations ranging from 1×10⁴ to 1×10⁸ cells ml⁻¹.</p