Quality assurance plan for the Objective Supply Capability Adaptive Redesign (OSCAR) project

This document establishes the Quality Assurance Plan (QAP) for the National Guard Bureau Objective Supply Capability Adaptive Redesign (OSCAR) project activities under the Oak Ridge National Laboratory (ORNL) management. It defines the requirements and assigns responsibilities for ensuring, with a high degree of confidence, that project objectives will be achieved as planned. The QAP outlined herein is responsive to and meets the Quality Assurance Program standards for the U.S. Department of Energy (DOE), Lockheed Martin Energy Research Corporation and ORNL and the ORNL Computing, Robotics, and Education Directorate (CRE). This document is intended to be in compliance with DOE Order 5700.6C, Quality Assurance Program, and the ORNL Standard Practice Procedure, SPP X-QA-8, Quality Assurance for ORNL Computing Software. This standard allows individual organizations to apply the stated requirements in a flexible manner suitable to the type of activity involved. Section I of this document provides an introduction to the OSCAR project QAP; Sections 2 and 3 describe the specific aspects of quality assurance as applicable to the OSCAR project. Section 4 describes the project approach to risk management. The Risk Management Matrix given in Appendix A is a tool to assess, prioritize, and prevent problems before they occur. Therefore, the matrix will be reviewed and revised on a periodic basis

ADAPTIVE SAMPLING IN DIGITAL CONTROL

This study provides a set of design curves from which effective digital control for an experiment can be obtained and at the same time efficient use of a digital computer shared with other experiments can be realized. The state variable technique is used as both a design method for obtaining the design curves for adaptive sampling and for predicting the states for control of the process. The adaptive sampling technique is defined to be that choice of constant prediction period that will satisfy control specifications over a specified range of system conditions. The complete range of system operation is divided into a number of classes for which criteria can be devised for determining when the system is in each class. In each class the prediction period is no smaller than that required over the range of the operation of the class. The objective of the adaptive sampling is to use the digital computer for control as little as possible yet maintain the system control specifications.</p

Spectrum of antibacterial activity and mode of action of a novel tris-stilbene bacteriostatic compound

The spectrum of activity and mode of action of a novel antibacterial agent, 135C, was investigated using a range of microbiological and genomic approaches. Compound 135C was active against Gram-positive bacteria with MICs for Staphylococcus aureus ranging from 0.12–0.5 μg/ml. It was largely inactive against Gram-negative bacteria. The compound showed bacteriostatic activity in time-kill studies and did not elicit bacterial cell leakage or cell lysis. Checkerboard assays showed no synergy or antagonism when 135C was combined with a range of other antibacterials. Multi-step serial passage of four S. aureus isolates with increasing concentrations of 135C showed that resistance developed rapidly and was stable after drug-free passages. Minor differences in the fitness of 135C-resistant strains and parent wildtypes were evident by growth curves, but 135C-resistant strains did not show cross-resistance to other antibacterial agents. Genomic comparison of resistant and wildtype parent strains showed changes in genes encoding cell wall teichoic acids. 135C shows promising activity against Gram-positive bacteria but is currently limited by the rapid resistance development. Further studies are required to investigate the effects on cell wall teichoic acids and to determine whether the issue of resistance development can be overcome

Influence of oxidation on fatigue crack initiation and propagation in turbine disc alloy N18

Fatigue crack initiation and propagation behaviour in subsolvus heat treated turbine disc alloy N18 has been assessed in air and vacuum at 650 and 725oC under three-point loading. Fatigue crack initiation processes have been evaluated using single edge U-notch specimens under a 1-1-1-1 trapezoidal loading waveform along with interrupted tests at 650oC to allow intermittent observations of the notch surface. The results show apparent grain boundary (GB) oxidation can occur under an oxygen partial pressure of 10-2?10-3Pa. Cracks mainly initiate from grain boundaries or ?/?? interfaces due to the formation and subsequent cracking of Cr-rich and/or Co-rich oxides, and occasionally initiate from surface pores. Fatigue life in these tests appears to be dominated by this crack initiation process and is significantly reduced by increasing temperature and/or application of an oxidizing environment. Crack growth tests conducted under 1-1-1-1 and 1-20-1-1 loading waveforms indicate that oxidation significantly degrades the crack growth resistance of N18 and is associated with more intergranular fracture surface features. Additional oxidation effects on propagation caused by higher temperature or prolonging dwell time appear limited, whereas a prolonged dwell period seems to instead promote additional creep process, which further enhance crack growth, especially at higher temperature

Nutrient‐dependent allometric plasticity in a male‐diphenic mite

Male secondary sexual traits often scale allometrically with body size. These allometries can be variable within species and may shift depending on environmental conditions, such as food quality. Such allometric plasticity has been hypothesized to initiate local adaptation and evolutionary diversification of scaling relationships, but is under-recorded, and its eco-evolutionary effects are not well understood. Here, we tested for allometric plasticity in the bulb mite (Rhizoglyphus robini), in which large males tend to develop as armed adult fighters with thickened third legs, while small males become adult scramblers without thickened legs. We first examined the ontogenetic timing for size- and growth-dependent male morph determination, using experimentally amplified fluctuations in growth rate throughout juvenile development. Having established that somatic growth and body size determine male morph expression immediately before metamorphosis, we examined whether the relationship between adult male morph and size at metamorphosis shifts with food quality. We found that the threshold body size for male morph expression shifts toward lower values with deteriorating food quality, confirming food-dependent allometric plasticity. Such allometric plasticity may allow populations to track prevailing nutritional conditions, potentially facilitating rapid evolution of allometric scaling relationships.Environmental Biolog