53 research outputs found
Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of SmaI macrorestriction fragments: a multicenter study
Get PDFTwenty well-characterized isolates of methicillin-resistant Staphylococcus
aureus were used to study the optimal resolution and interlaboratory
reproducibility of pulsed-field gel electrophoresis (PFGE) of DNA
macrorestriction fragments. Five identical isolates (one PFGE type), 5
isolates that produced related PFGE subtypes, and 10 isolates with unique
PFGE patterns were analyzed blindly in 12 different laboratories by
in-house protocols. In several laboratories a standardized PFGE protocol
with a commercial kit was applied successfully as well. Eight of the
centers correctly identified the genetic homogeneity of the identical
isolates by both the in-house and standard protocols. Four of 12
laboratories failed to produce interpretable data by the standardized
protocol, due to technical problems (primarily plug preparation). With the
five rel
Cloning and Characterization of a cDNA Encoding Calcium/Calmodulin-Dependent Glutamate Decarboxylase from Scutellaria Baicalensis
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The Regulation of Somatic Embryo Development in Plant Cell Cultures: Suspension Culture Techniques and Hormone Requirements
No full textEffects of temperature and oxygen depletion on metabolic rates of tomato and carrot cell cultures and cuttings measured by calorimetry
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