Water and Nutrient Outflow From Contrasting Lodgepole Pine Forests in Wyoming

Factors affecting water and nutrient outflow beyond the rooting zone were studied during a 3—yr period, using data from eight contrasting stands of lodgepole pine (Pinus contorta ssp. latifolia) forest in southeastern Wyoming and the output of a hydrologic simulation model (H2OTRANS) based on tree physiology. Nutrient outflow during a specific time period was estimated by multiplying simulated water outflow times element concentrations in the soil solution, the latter determined from samples collected periodically near the bottom of the rooting zone. Estimates of actual evapotranspiration (ET) for the period from early spring to late fall ranged from 21 to 53 cm, which was 33—95% (x = 73%) of total annual precipitation. For all stands and years, transpiration accounted for 50—61% of ET, and 9—44% of the transpiration occurred during the spring drainage period (vernal transpiration, VT). Estimated VT and outflow varied considerably among the stands, with VT accounting for 4—20% of the snow water. Outflow occurred only during the snow melt period and accounted for 0—80% of the snow water. Snow water equivalent varied annually by 300% or more. Nutrient outflow from the different stands also varied greatly. Ratios between simulated annual outflow and atmospheric inputs (bulk precipitation) were consistently \u3e1.0 for Ca, Na, and Mg; were consistently \u3c1.0 for N; and ranged from 0.3 to 2.0 for P and from 0.2 to 3.3 for K. Much of the variability in water and nutrient outflow can be attributed to the degree of biotic control, with water outflow affected by a different combination of factors than nutrient outflow. H2OTRANS was used to simulate the effects on outflow of different snow water equivalents and different total leaf areas. One result of the simulations was that nitrogen appears to be retained even at the highest levels of water outflow. Another was that increases in water outflow following reduction in leaf area were proportional to the leaf area removed. The results indicate that stands differing in site or habitat type experience different rates of water and element losses at different times during the snow melt season, and contribute differentially to streamwater quality and hydrograph shape. Factors affecting outflow are discussed in the context of successional trends, common perturbations including timber harvest, and hypotheses pertaining to nutrient conservation in terrestrial ecosystems. Nutrient retention in the snow—dominated lodgepole pine ecosystem appears to be primarily dependent on evergreen leaf area, duration of the VT period, and high carbon/nutrient ratios of the forest floor. Net losses of limiting nutrients probably occur primarily in pulses after abiotic perturbations such as fire

Sustaining Educational Reforms in Introductory Physics

While it is well known which curricular practices can improve student performance on measures of conceptual understanding, the sustaining of these practices and the role of faculty members in implementing these practices are less well understood. We present a study of the hand-off of Tutorials in Introductory Physics from initial adopters to other instructors at the University of Colorado, including traditional faculty not involved in physics education research. The study examines the impact of implementation of Tutorials on student conceptual learning across eight first-semester, and seven second-semester courses, for fifteen faculty over twelve semesters, and includes roughly 4000 students. It is possible to demonstrate consistently high, and statistically indistinguishable, student learning gains for different faculty members; however, such results are not the norm, and appear to rely on a variety of factors. Student performance varies by faculty background - faculty involved in, or informed by physics education research, consistently post higher student learning gains than less-informed faculty. Student performance in these courses also varies by curricula used - all semesters in which the research-based Tutorials and Learning Assistants are used have higher student learning gains than those semesters that rely on non-research based materials and do not employ Learning Assistants.Comment: 21 pages, 4 figures, and other essential inf

Transpiration From 100-yr-old Lodgepole Pine Forests Estimated with Whole-Tree Potometers

Whole—tree potometers were used to estimate transpiration from two contrasting stands of 100—yr—old lodgepole pine (Pinus contorta Dougl. var. latifolia Engelm.) forest growing on the same site in southeastern Wyoming. Although one stand had nearly seven times as any trees per hectare and 29% less biomass, both stands had nearly identical leaf area indices (7.3 and 7.1) and clear—day transpiration rates (3.3 and 3.4 mm/d). Individual tree basal area and maximum observed 24—h uptake were highly correlated, with the largest trees (20—26 cm dbh) transpiring 40—44 L on clear days in early summer. Maximum observed hourly uptake for the larger trees was 2.5—3.5 L, with total nighttime uptake being about 12% of 25—h uptake. On overcast days potometer uptake was reduced by 30—44%; during rainy periods uptake was reduced to nearly zero. The results are compared to data obtained with different methods by other investigators, with the conclusion that whole—tree potometers can be a useful tool for studies on tree water relations and for estimating short—term forest transpiration when leaf water potential is not limiting leaf conductance

Bright Giant Pulses from the Crab Nebula Pulsar: Statistical Properties, Pulse Broadening and Scattering due to the Nebula

We report observations of Crab giant pulses made with the Australia Telescope Compact Array and a baseband recorder system, made simultaneously at two frequencies, 1300 and 1470 MHz. These observations were sensitive to pulses with amplitudes \ga 3 kJy and widths \ga 0.5 $\mu$s. Our analysis led to the detection of more than 700 such bright giant pulses over 3 hours, and using this large sample we investigate their amplitude, width, arrival time and energy distributions. The brightest pulse detected in our data has a peak amplitude of $\sim$ 45 kJy and a width of $\sim$ 0.5 $\mu$s, and therefore an inferred brightness temperature of $\sim 10^{35}$ K. The duration of giant-pulse emission is typically $\sim$1 $\mu$s, however it can also be as long as 10 $\mu$s. The pulse shape at a high time resolution (128 ns) shows rich diversity and complexity in structure and is marked by an unusually low degree of scattering. We discuss possible implications for scattering due to the nebula, and for underlying structures and electron densities.Comment: 8 pages, 8 figures, Accepted for publication in Ap

Expression of Integrin-αE by Mucosal Mast Cells in the Intestinal Epithelium and Its Absence in Nematode-Infected Mice Lacking the Transforming Growth Factor-β1-Activating Integrin αvβ6

Peak intestinal mucosal mast cell (MMC) recruitment coincides with expulsion of Trichinella spiralis, at a time when the majority of the MMCs are located within the epithelium in BALB/c mice. Although expression of integrin-α(E)β(7) by MMCs has not been formally demonstrated, it has been proposed as a potential mechanism to account for the predominantly intraepithelial location of MMCs during nematode infection. Co-expression of integrin-α(E)β(7) and the MMC chymase mouse mast cell protease-1, by mouse bone marrow-derived mast cells, is strictly regulated by transforming growth factor (TGF)-β(1). However, TGF-β(1) is secreted as part of a latent complex in vivo and subsequent extracellular modification is required to render it biologically active. We now show, for the first time, that intraepithelial MMCs express integrin-α(E)β(7) in Trichinella-infected BALB/c and S129 mice. In S129 mice that lack the gene for the integrin-β(6) subunit and, as consequence, do not express the epithelial integrin-α(v)β(6), integrin-α(E) expression is virtually abolished and recruitment of MMCs into the intestinal epithelium is dramatically reduced despite significant overall augmentation of the MMC population. Because a major function of integrin-α(v)β(6) is to activate latent TGF-β(1,) these findings strongly support a role for TGF-β(1) in both the recruitment and differentiation of murine MMCs during nematode infection

Stops making sense: translational trade-offs and stop codon reassignment

Background Efficient gene expression involves a trade-off between (i) premature termination of protein synthesis; and (ii) readthrough, where the ribosome fails to dissociate at the terminal stop. Sense codons that are similar in sequence to stop codons are more susceptible to nonsense mutation, and are also likely to be more susceptible to transcriptional or translational errors causing premature termination. We therefore expect this trade-off to be influenced by the number of stop codons in the genetic code. Although genetic codes are highly constrained, stop codon number appears to be their most volatile feature. Results In the human genome, codons readily mutable to stops are underrepresented in coding sequences. We construct a simple mathematical model based on the relative likelihoods of premature termination and readthrough. When readthrough occurs, the resultant protein has a tail of amino acid residues incorrectly added to the C-terminus. Our results depend strongly on the number of stop codons in the genetic code. When the code has more stop codons, premature termination is relatively more likely, particularly for longer genes. When the code has fewer stop codons, the length of the tail added by readthrough will, on average, be longer, and thus more deleterious. Comparative analysis of taxa with a range of stop codon numbers suggests that genomes whose code includes more stop codons have shorter coding sequences. Conclusions We suggest that the differing trade-offs presented by alternative genetic codes may result in differences in genome structure. More speculatively, multiple stop codons may mitigate readthrough, counteracting the disadvantage of a higher rate of nonsense mutation. This could help explain the puzzling overrepresentation of stop codons in the canonical genetic code and most variants

Plutonium Migration during the Leaching of Cemented Radioactive Waste Sludges

One of the most challenging components of the UK nuclear legacy is Magnox sludge, arising from the corrosion of Mg alloy-clad irradiated metallic U fuel that has been stored in high pH ponds. The sludges mainly comprise Mg hydroxide and carbonate phases, contaminated with fission products and actinides, including Pu. Cementation and deep geological disposal is one option for the long-term management of this material, but there is a need to understand how Pu may be leached from the waste, if it is exposed to groundwater. Here, we show that cemented Mg(OH)2 powder prepared with Pu(IV)aq is altered on contact with water to produce a visibly altered ‘leached zone’, which penetrates several hundred microns into the sample. In turn, this zone shows slow leaching of Pu, with long-term leaching rates between 1.8–4.4 × 10−5% of total Pu per day. Synchrotron micro-focus X-ray fluorescence mapping identified decreased Pu concentration within the ‘leached zone’. A comparison of micro-focus X-ray absorption spectroscopy (µ-XAS) spectra collected across both leached and unleached samples showed little variation, and indicated that Pu was present in a similar oxidation state and coordination environment. Fitting of the XANES spectra between single oxidation state standards and EXAFS modeling showed that Pu was present as a mixture of Pu(IV) and Pu(V). The change in Pu oxidation from the stock solution suggests that partial Pu oxidation occurred during sample ageing. Similarity in the XAS spectra from all samples, with different local chemistries, indicated that the Pu oxidation state was not perturbed by macro-scale variations in cement chemistry, surface oxidation, sample aging, or the leaching treatment. These experiments have demonstrated the potential for leaching of Pu from cementitious waste forms, and its underlying significance requires further investigation

Plutonium Migration during the Leaching of Cemented Radioactive Waste Sludges

One of the most challenging components of the UK nuclear legacy is Magnox sludge, arising from the corrosion of Mg alloy-clad irradiated metallic U fuel that has been stored in high pH ponds. The sludges mainly comprise Mg hydroxide and carbonate phases, contaminated with fission products and actinides, including Pu. Cementation and deep geological disposal is one option for the long-term management of this material, but there is a need to understand how Pu may be leached from the waste, if it is exposed to groundwater. Here, we show that cemented Mg(OH)2 powder prepared with Pu(IV)aq is altered on contact with water to produce a visibly altered ‘leached zone’, which penetrates several hundred microns into the sample. In turn, this zone shows slow leaching of Pu, with long-term leaching rates between 1.8–4.4 × 10−5% of total Pu per day. Synchrotron micro-focus X-ray fluorescence mapping identified decreased Pu concentration within the ‘leached zone’. A comparison of micro-focus X-ray absorption spectroscopy (µ-XAS) spectra collected across both leached and unleached samples showed little variation, and indicated that Pu was present in a similar oxidation state and coordination environment. Fitting of the XANES spectra between single oxidation state standards and EXAFS modeling showed that Pu was present as a mixture of Pu(IV) and Pu(V). The change in Pu oxidation from the stock solution suggests that partial Pu oxidation occurred during sample ageing. Similarity in the XAS spectra from all samples, with different local chemistries, indicated that the Pu oxidation state was not perturbed by macro-scale variations in cement chemistry, surface oxidation, sample aging, or the leaching treatment. These experiments have demonstrated the potential for leaching of Pu from cementitious waste forms, and its underlying significance requires further investigation