Summary of results of the species delimitation analysis.

<p>Summary of results of the species delimitation analysis.</p

Phylogeny of iguanian lizards with emphasis on <i>Polychrus</i>.

<p>Maximum clade credibility tree obtained from a Bayesian analysis of 62 specimens, three mitochondrial genes (12S, 16S, ND2) and one nuclear gene (RAG1). Numbers above branches correspond to Bayesian posterior probability (PP) values. For specimens of <i>Polychrus</i>, voucher number and country of origin are indicated. GenBank accession numbers along with more detailed locality data are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0178139#pone.0178139.t001" target="_blank">Table 1</a> for all specimens included in this tree. Photographs: <i>P</i>. <i>peruvianus</i> (top; C. Koch), <i>P</i>. <i>femoralis</i> (bottom; F. Ayala-Varela).</p

Primers and protocols used for amplification and sequencing reactions.

<p>Primers and protocols used for amplification and sequencing reactions.</p

Distribution of <i>Polychrus</i> lizards.

<p>Bold-black delimited symbols represent localities from which DNA was used in our analyses. In accordance with Murphy et al. (2017) we restrict the name <i>P</i>. <i>marmoratus</i> to the populations in Guyana and Suriname and designate remaining populations as the “<i>marmoratus</i>” group. Except for <i>P</i>. <i>femoralis</i>, most locality data derived from the literature and unverified VertNet records.</p

16S gene tree of <i>Polychrus</i>.

<p>Maximum clade credibility tree obtained from a Starbeast analysis of 55 specimens. Numbers above branches correspond to Bayesian posterior probability (PP) values. Taxon name, voucher number, and locality are indicated for each terminal. Node numbers correspond to both currently recognized and putative species as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0178139#pone.0178139.t004" target="_blank">Table 4</a>.</p

<i>Polychrus</i> species tree.

<p>Maximum clade credibility tree obtained from a Starbeast analysis of 31 specimens, three mitochondrial genes (12S, 16S, ND2) and one nuclear gene (RAG1). Numbers above branches correspond to Bayesian posterior probability (PP) values. Branch lengths units are expected substitutions per site.</p

Vouchers, locality data, and GenBank accession numbers of taxa and gene regions included in this study.

<p>Vouchers, locality data, and GenBank accession numbers of taxa and gene regions included in this study.</p

Estimated ages of <i>Polychrus</i> from the literature.

<p>Estimated ages of <i>Polychrus</i> from the literature.</p