Additional file 4: Figure S3. of Enhanced in vitro osteogenic differentiation of human fetal MSCs attached to 3D microcarriers versus harvested from 2D monolayers

Kinetics of osteogenic gene expression during osteogenic differentiation of S27 hfMSC in PCL-TCP scaffold cultures. (A) Cultures seeded with 2D monolayer-harvested (2D MNL-harv) and microcarrier-harvested (3D MC-harv) cells, (B) Cultures seeded with microcarrier-harvested (2D MNL-harv) and microcarrier-bound (3D MC-bound) cells. Two-way repeated measures ANOVA with post-hoc Tukey correction was performed between 3D MC-harv, 2D MNL-harv and 3D MC-bound cells using Graphpad, (*p < 0.05 and at least a 2-fold difference in the means). Of the multiple comparisons performed, data representing a single comparison is shown: (A) 3D MC-harv vs 2D MNL-harv, (B) 3D MC-bound vs 2D MNL-harv. (TIFF 544 kb

Additional file 5: Table S1. of Enhanced in vitro osteogenic differentiation of human fetal MSCs attached to 3D microcarriers versus harvested from 2D monolayers

List of Taqman Gene Expression Assay IDs for genes investigated by qPCR in this study. (DOCX 13 kb

Additional file 1: Figure S1. of A method for near full-length amplification and sequencing for six hepatitis C virus genotypes

Successful amplification of near full-length HCV genomes from multiple genotypes. Amplicons (~9.2 kb) were run on an agarose gel (0.8 %) and visualized on a Gel Doc molecular imager (Bio-Rad); M represents the DNA marker HyperLadder 1 (Bioline). Lanes 1 to 8 represent GT1a amplicons. Lanes 9 to 11 represent GT1b amplicons. Lanes 12 to 16 represent GT3a amplicons. All amplicons were purified and successfully sequenced on the Illumina platform, including the faintly visible band in lane 7. (PDF 2448 kb