2 research outputs found
Primary sequence of the glucanase gene from Oerskovia xanthineolytica. Expression and purification of the enzyme from Escherichia coli.
A 2.7-kilobase fragment of DNA from Oerskovia xanthineolytica containing the gene for a beta-1,3-glucanase has been isolated and its complete nucleotide sequence determined. The sequence was found to contain two large open reading frames. Purification of the mature native enzyme and subsequent amino-terminal sequencing defined the glucanase gene in one reading frame which potentially encodes a protein of 548 amino acids. We have expressed this glucanase gene in Escherichia coli under control of the lacUV5 promoter and found the product to be secreted into the periplasm as a mature enzyme of about the same molecular weight as that of the native protein. The recombinant enzyme was purified to near homogeneity by a single step of high performance liquid chromatography. The ability of the recombinant enzyme to digest beta-glucan substrates and to lyse viable yeast cells was found to be indistinguishable from that of the native protein. Deletion of the cysteine-rich carboxyl-terminal 117 amino acids of the enzyme, which also contain two duplicated segments, abolished the lytic activity but did not significantly affect the glucanase function of the protein. The possible involvement of this domain in interaction with the yeast cell wall is discussed
Recommended from our members
A NOVEL GENE TRANSFER SYSTEM FOR MAMMALIAN CELLS.
Productive infection of mouse cells with polyoma virus yields mainly two types of particles: Complete virions and empty capsids. Empty polyoma capsids have been shown to be capable of interacting with DNA, in vitro, to form what has been referred to as polyoma-like particles (PLP). The particles are stable in high concentrations of salt and contain DNA protected by the capsid against the action of pancreatic DNase. The development of PLP into a gene transfer vehicle is the subject of the investigations described in the present dissertation. The approach has been to first, characterize the process of PLP formation and second, determine whether the genetic information contained in a specific DNA fragment and assembled into PLP in vitro can be transferred to cells and subsequently be expressed. In terms of PLP characteristics, the experimental results described in this dissertation show that the DNA extracted from PLP is heterogeneous in size. It has a mean molecular weight of 1.2 x 10⁶ with a standard deviation of ±0.5 x 10⁶. In addition, analysis of PLP DNA with restriction endonucleases revealed that a specific primary sequence or higher order structure is not required for PLP formation. Either linear, circular or supercoiled polyoma DNA, as well as, single-stranded DNA, rRNA and the synthetic homopolymers poly(dA).poly(dT) and poly(dG).poly(dC) can be used for PLP formation. Transfer of genetic information by PLP has been accomplished by using a restriction fragment containing the transforming sequences of polyoma DNA as a model gene. This fragment of polyoma DNA, which consists of 1,831 base pairs (approximately 1.2 x 10⁶ daltons) and extends clockwise from the BclI site to the EcoRI site on the conventional polyoma map, causes the induction of the transformed phenotype in rat cells grown in culture. Infection of rat F111 cells by PLP, containing this DNA fragment, results in DNA-mediated oncogenic transformation of the cells as indicated by the formation of dense foci. This gene transfer activity of PLP is shown to be 50 to 150 times more efficient than the widely used calcium phosphate coprecipitation method of introducing DNA into mammalian cells