Additional file 6: Figure S6. of Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors

Reduced IGF1R but not downstream signalling in human astrocytes derived from temporal lobe resections using MAB391. Human astrocytes treated with MAB391 for 24 h. A) Immunoblots for IGF1R, pAkt, total Akt, pP44/42 MAPK and total p44/42 MAPK. α-tubulin was used as a loading control for blots. Molecular weight markers are indicated (kDa). (B) Bar charts showing quantification of blots, pAkt was normalised to Akt/tubulin. students unpaired t-test, **** p < 0.0001. (TIFF 1949 kb

() Effect of time of preincubation of ATL proteins with MGMT substrate DNA on MGMT activity (triangles, Atl1; squares, eATL; open symbols, ATL proteins alone; filled symbols, after addition of MGMT; filled circle, MGMT alone)

<p><b>Copyright information:</b></p><p>Taken from "A novel DNA damage recognition protein in "</p><p>Nucleic Acids Research 2006;34(8):2347-2354.</p><p>Published online 5 May 2006</p><p>PMCID:PMC1458281.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Effect of heat on ATL activity (triangles, Atl1; squares, eATL; continuous line, before heating; broken line, after heating; open circle, MGMT activity after heating). () Effect of preincubation with Atl1 on the ability of oligonucleotides containing -benzylguanine (filled diamonds) or -(4-bromothenyl)guanine (open diamonds) to inactivate MGMT. Inhibition of MGMT by Atl1is indicated by open triangles