12 research outputs found
Diagnostic imaging of cervical intraepithelial neoplasia based on hematoxylin and eosin fluorescence
Background Pathological classification of cervical intraepithelial neoplasia (CIN) is problematic as it relies on subjective criteria. We developed an imaging method that uses spectroscopy to assess the fluorescent intensity of cervical biopsies derived directly from hematoxylin and eosin (H&E) stained tissues. Methods Archived H&E slides were identified containing normal cervical tissue, CIN I, and CIN III cases, from a Community Hospital and an Academic Medical Center. Cases were obtained by consensus review of at least 2 senior pathologists. Images from H&E slides were captured first with bright field illumination and then with fluorescent illumination. We used a Zeiss Axio Observer Z1 microscope and an AxioVision 4.6.3-AP1 camera at excitation wavelength of 450â490 nm with emission captured at 515â565 nm. The 32-bit grayscale fluorescence images were used for image analysis. Results We reviewed 108 slides: 46 normal, 33 CIN I and 29 CIN III. Fluorescent intensity increased progressively in normal epithelial tissue as cells matured and advanced from the basal to superficial regions of the epithelium. In CIN I cases this change was less prominent as compared to normal. In high grade CIN lesions, there was a slight or no increase in fluorescent intensity. All groups examined were statistically different. Conclusion Presently, there are no markers to help in classification of CIN I-III lesions. Our imaging method may complement standard H&E pathological review and provide objective criteria to support the CIN diagnosis
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Alaska Natural Gas Pipelines: Interaction of the Natural Gas and Steel Markets
In 1976, Congress approved legislation to establish the regulatory framework for building a pipeline to bring natural gas from the Alaska North Slope to the lower 48 states. This report focuses on two major issues or uncertainties that may serve as economic constraints on a capital investment undertaking of this major scale
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Minerals Price Increases and Volatility: Causes and Consequences
This report discusses China's efforts to improve and increase its access to foreign mineral resources, which may have the effect of raising prices for U.S. domestic industrial users. The report examines in detail the relationship between prices, production, and availability of selected metal minerals essential to the U.S. economy. It focuses on iron ore, aluminum (bauxite/alumina), copper, manganese, molybdenum (moly), zinc, platinum group metals (PGMs), and uranium