1 research outputs found
Recombinant Human Fibrinogen That Produces Thick Fibrin Fibers with Increased Wound Adhesion and Clot Density
Human fibrinogen is a biomaterial used in surgical tissue
sealants,
scaffolding for tissue engineering, and wound healing. Here we report
on the post-translational structure and functionality of recombinant
human FI (rFI) made at commodity levels in the milk of transgenic
dairy cows. Relative to plasma-derived fibrinogen (pdFI), rFI predominately
contained a simplified, neutral carbohydrate structure and >4-fold
higher levels of the γ′-chain transcriptional variant
that has been reported to bind thrombin and Factor XIII. In spite
of these differences, rFI and pdFI were kinetically similar with respect
to the thrombin-catalyzed formation of protofibrils and Factor XIIIa-mediated
formation of cross-linked fibrin polymer. However, electron microscopy
showed rFI produced fibrin with much thicker fibers with less branching
than pdFI. In vivo studies in a swine liver transection model showed
that, relative to pdFI, rFI made a denser, more strongly wound-adherent
fibrin clot that more rapidly established hemostasis