Summary of Materials and Methods.

<p>Summary of Materials and Methods.</p

Boxplots representing the median binding of each isolate and reference strain to mucin from the UC colon compared to controls.

<p>Direct comparison of the median binding of isolates of <i>A</i>. <i>mucinihpila</i> (a). Direct comparison of median binding of isolates of <i>Desulfovibrio</i> spp. (b).</p

Median binding values of each isolate to mucin from controls and the UC colon and Kruskal-Wallis tests comparing the binding of clinical isolates to that of the reference strain for <i>A</i>. <i>muciniphila</i> and <i>Desulfovibrio</i> spp.

<p>Significant values are highlighted in bold text. FU corresponds to measure of bacterial binding in fluorescent units. IQR corresponds to inter quartile range.</p><p>Median binding values of each isolate to mucin from controls and the UC colon and Kruskal-Wallis tests comparing the binding of clinical isolates to that of the reference strain for <i>A</i>. <i>muciniphila</i> and <i>Desulfovibrio</i> spp.</p

Boxplots illustrating the binding of clinical isolates and reference strains to mucin from different sources.

<p>Median binding of isolates of <i>A</i>. <i>muciniphila</i> to control mucin (a) Median binding of isolates of <i>A</i>. <i>muciniphila</i> to UC mucin (b) Median binding of isolates of <i>Desulfovibrio</i> spp. to control mucin (c) Median binding of isolates of <i>Desulfovibrio</i> spp. to UC mucin (d).</p

A preliminary study examining the binding capacity of akkermansia muciniphila and desulfovibrio spp., to colonic mucin in health and ulcerative colitis

Akkermansia muciniphila and Desulfovibrio spp. are commensal microbes colonising the mucus gel layer of the colon. Both species have the capacity to utilise colonic mucin as a substrate. A. muciniphila degrades colonic mucin, while Desulfovibrio spp. metabolise the sulfate moiety of sulfated mucins. Altered abundances of these microorganisms have been reported in ulcerative colitis (UC). However their capacity to bind to human colonic mucin, and whether this binding capacity is affected by changes in mucin associated with UC, remain to be defined

Comparison of the glycosylation profiles of human colonic mucins generated from lectin profiling of the mucin microarray.

<p>The maximum binding for colonic mucins is 8,335 RFU. The highest intensity binding is represented by red, followed by orange, yellow and white.</p

Comparison of bacterial binding to control and UC mucin as determined by the Mann-Whitney U test.

<p>Significant values are highlighted in bold text. FU corresponds to measure of bacterial binding in fluorescent units. IQR corresponds to inter quartile range.</p><p>Comparison of bacterial binding to control and UC mucin as determined by the Mann-Whitney U test.</p