Histology and histomorphometry ISA file (Metadata)

<p>The file contains (i) general information on the experiment and the protocols related to the dissection and histology and histomorphometry evaluations on the “i_Organism” tab, (ii) information on each animal in the “s_Organism” tab, (iii) information on the samples that were measured in the “s_DP_Histo” tab, (iv) the links between the metadata and the histopathology measurements given in the Data records 15 in the "a_DP_Histopathology” tab, (v) the links between<br>the metadata and the histomorphometry measurements given in the Data records 15<br>in the “a_DP_Histomorphometry” tab</p

Body weight ISA file (Metadata)

<p>The file contains (i) general information on the experiment and the protocols related to exposure and body weight measurement on the “i_Organism” tab, (ii) information on each animal in the “s_Organism” tab, (iii) information on the body weight measurement in the “a_Organism_BodyWeight”.</p

Hematology ISA file (Metadata)

<p>The file contains (i) general information on the experiment and the protocols related to the dissection and hematology measurement on the “i_Organism” tab, (ii) information on each animal in the “s_Organism” tab, (iii) information on the samples that were measured in the “s_DP_Hematology” tab, and (iv) information on the measurements performed for hematology in the “a_DP_Hematology” tab.</p

Lung function Raw Data

<p>Three consecutive perturbations were performed to record acceptable measurements (coefficient of determination >0.95) for each individual subject. FlexiVent software (SCIREQ) was used to analyze and calculate lung mechanics parameters. Measurements arepresented as a data matrix. Briefly, it has the animal number information on the first row and the exposure group information in the second row. Then, the first column has the name of the lung function parameter measured.</p

The SETD7 knock-down affects the cell cycle profile of differentiating cells.

<p>(A) Cell cycle profile of undifferentiated and after 15 days of <i>in vitro</i> differentiation of ES[4] transduced with a non target shRNA (shSCR) and a shRNA that targets SETD7 (shSETD7). (B) Mean of the percentage of cells in each phase of the cell cycle in three independent differentiation experiments. *Differences in the percentage of cells in S-phase between shSETD7 and shSCR in differentiated cells was found significant at a p-value<0.05.</p

SETD7 interacting proteins identified by mass spectrometry.

<p>SETD7 interacting proteins identified by mass spectrometry.</p

Body weight Raw Data

<p>The file shows the animal number information on the first row and the exposure group information in the second row. Then, the first column has the study day information, and each body weight recorded (in grams) is recorded in corresponding cells.</p

Residues modified by SETD7 in H1.0 and H1.4 found by mass spectrometry.

<p>Residues modified by SETD7 in H1.0 and H1.4 found by mass spectrometry.</p

The SETD7 knock-down causes defects in the silencing of pluripotency genes.

<p>(A) Western blot showing the levels of OCT4 at different days during the in vitro differentiation of ES[4] transduced with a non target shRNA (shSCR) and a shRNA that targets SETD7 (shSETD7) (B) Immunolocalization of SOX2 (green) and OCT4 (red) expression at day 4 and day 15 of in vitro differentiation of ES[4] transduced with a non target shRNA (shSCR) and a shRNA that targets SETD7 (shSETD7) (C) Quantification of the percentage of embryoid bodies negative or positive for OCT4 staining at day 4 and day 7 of differentiation of cells treated with vehicle (DMSO), 1μM or 5μM PFI-2. (D) Immunolocalization of OCT4 (red) in embryoid bodies at day 4 and day 7 of in vitro differentiation of ES[4] treated with vehicle (DMSO) or 5μM PFI-2.</p

BALF FACS analyses reports

<p>pdf reports of all BALF FACS analyses</p