Splicing of porcine <i>NR3C1</i> shows no differences associated with SNP c.1829C>T.

<p>Splicing of the glucocorticoid receptor alpha isoform was examined using reverse-transcription PCR and cDNA from adrenal (A) and pituitary gland (B) of three individuals alternative homozygous for SNP c.1829C>T. Exons (denoted as E) of porcine <i>NR3C1</i> covered by the PCR amplicons are indicated above the corresponding PCR products.</p

Association of the three identified missense SNPs in porcine NR3C1 with plasma cortisol level (ng/ml) in three different commercial populations.

1<p>LR-German Landrace, PiF1-(Pietrain × (German Large White × German Landrace), LW-German Large White.</p>2<p>Least-squares means (LSM) with different superscripts ^{A,B; a,b} differ significantly at p≤0.01 and 0.05 respectively.</p>3<p>Allele substitution effect in fractions of phenotypic standard deviation.</p>4<p>Phenotypic variance in percent explained by the SNP.</p

Association of the three identified missense SNPs in porcine <i>NR3C1</i> with weight of the left adrenal gland (g) in three different commercial populations.

1<p>LR-German Landrace, PiF1-(Pietrain × (German Large White × German Landrace), LW-German Large White.</p>2<p>Least-squares means (LSM) with different superscripts ^A,B,C differ significantly at p≤0.01.</p>3<p>Allele substitution effect in fractions of phenotypic standard deviation.</p>4<p>Phenotypic variance in percent explained by the SNP.</p

Manhattan plot of the genome-wide association analysis of plasma cortisol levels (A) and adrenal weight (B).

<p>The red line indicates the significance threshold corresponding to a genome-wide false discovery rate q-value ≤0.05.</p

Position of the detected single nucleotide polymorphisms in porcine <i>NR3C1.</i>

<p>Exons are represented by numbered boxes. Coding region is highlighted by coloring according to the encoded functional domain. Single nucleotide polymorphisms (SNP) and resulting amino acid substitutions are designated according to human genome variation society nomenclature. Synonymous and non-synonymous SNPs are represented by dashed and solid arrows, respectively.</p

The p.Ala610Val substitution increases sensitivity of porcine GR to glucocorticoids <i>in vitro</i>.

<p>Transcriptional activity of the two GR-alpha variants was investigated in COS-7 cells cotransfected with the pGL4.36 reporter construct expressing firefly luciferase under the control of the glucocorticoid-inducible mouse mammary tumor virus promoter after induction with dexamethasone at the indicated concentrations. (A) Fold induction of normalized luciferase expression in dexamethasone- vs. vehicle-treated cells. Significant differences (p<0.05) are indicated by an asterisk; (B) Estimated dose-response curve and EC50. Data are means ± SEM of four separate experiments performed in triplicate.</p

Allelic expression imbalance of porcine <i>NR3C1</i>.

<p>The box plots show the log₂-transformed ratios of the T allele compared to the C allele of SNP c.1829C>T as measured in genomic DNA (gDNA) and in cDNA obtained from hippocampus, hypothalamus, pituitary, and adrenal tissues. The right side of the graph shows corresponding means ± standard deviations and p-values of the test for allelic expression imbalance.</p