Apocynin: Molecular Aptitudes

Apocynin is a naturally occurring methoxy-substituted catechol, experimentally used as an inhibitor of NADPH-oxidase. It can decrease the production of superoxide (O2−) from activated neutrophils and macrophages while the ability of phagocytosis remains unaffected. The anti-inflammatory activity of apocynin has been demonstrated in a variety of cell and animal models of inflammation. Apocynin, after metabolic conversion, inhibits the assembly of NADPH-oxidase that is responsible for reactive oxygen species (ROS) production. It is, therefore, extensively used to reveal the role of this enzyme in cell and experimental models. Although some of the ROS serve as signaling molecules in the cells, excessive production is damaging and has been implicated to play an important role in the progression of many disease processes. This is why in many studies apocynin presents a promising potential treatment for some disorders; however, its utility with inflammatory diseases remains to be determined. Since its mode of action is not well defined, we tried to get a more precise insight into the mechanisms by which apocynin exerts its activity. Considering the anti-inflammatory activities of apocynin, we may conclude that this compound definitely deserves further study

Whiter Than White: The Art of Delaying Detection in Sexual Killers

The study investigated whether different types of sexual homicide perpetrators are more or less skilled at delaying detection. Using the newly proposed direct/indirect typology, the time of arrest, the frequency of specific precautions as well as the impact of forensic strategies used by the perpetrators were examined. The results indicated that the time from the killing to the arrest, as measured in days, was longer for the direct than the indirect sexual killers. Despite the fact that the direct aggressors were better at delaying detection, overall the indirect and the direct offenders did not differ in the frequency of use of most of the precautions. However, different forensic awareness strategies were more efficacious for the direct and the indirect offenders. These results are discussed in relation to the crime scripts for the two perpetrator groups

Sadism among sexual homicide offenders:Validation of the Sexual Sadism Scale

Sexual sadism is assumed to be a crucial factor in sexual homicide. Prevalence estimates vary greatly due to differences in the definition of sexual sadism. A nationwide sample of 350 male perpetrators who had committed a sexual homicide offense against a female 14 years of age or above in England or Wales was assessed based on archival records. Sexual sadism was assessed using the Sexual Sadism Scale (SeSaS). Item response theory (IRT) analyses were conducted focusing on the 2-parameter logistic model. The single-factor structure of the SeSaS Part 1 was tested using confirmatory factor analysis. Estimates of both internal consistency and interrater agreement were satisfactory to substantial. IRT analysis showed that the Part 1 items captured moderate to severe levels of the latent construct (i.e., theta levels >0). Based on the Posterior Probability of Diagnosis index, the prevalence of the disorder was estimated at 37% in the sample. The substantial correlation between the SeSaS Part 1 total score and original clinical diagnoses of sadism confirms the criterion validity of the scale. Exertion of control and infliction of torture were among the more informative items. In sum, the results support the usefulness of the SeSaS instrument for assessing forensically relevant forms of sadism

The developmental and criminal histories of subgroups of sexual murderers engaging, or not engaging, in post mortem sexual interference, compared to rapists

Purpose Identifying factors that may predict sexual aggression in the context of directly sexual murder, indirectly sexual murder, and non-fatal outcomes is necessary for advancing a field lacking a substantiated multifactorial theoretical model. Methods Eighty-nine sexual murderers engaging in post mortem sexual interference were compared to 92 non-post mortem sexual interference sexual murderers and 72 rapists on developmental factors, adult lifestyle, and criminal history. An overall model was built using a series of multinomial logistic regression analyses. Results Unlike rapists, both groups of sexual murderers experienced a lack of success in sexually intimate relationships. Perpetrators of post mortem sexual interference were rarely necrophiles, but having a history of sadistic behaviors or interests uniquely predicted sexual murder involving post mortem sexual interference. Chronic violent and sexual offending was characteristic of rapists. Psychopathy, measured using the screening version of the Psychopathy Checklist (Hart, Cox, & Hare, 1995), was not predictive of any outcome. Conclusion Results support criticism of existing theoretical models; that they do not apply to non-sadistic sexual murder. Findings are discussed in relation to gaps in theoretical understanding of sexual murder, and concerning implications for forensic policies and practice

A systematic review of the literature comparing male non-serial sexual killers and sexual aggressors: examining homogeneous and heterogeneous characteristics of these groups

This systematic review considers what is known about the characteristics of sexual killers and sexual aggressors. The aim was to identify which characteristics have consistently been shown to be similar (homogeneous) and which are different (heterogeneous) across these two groups. Ten studies, including approximately 300 sexual killers, were analysed. Only studies where the samples were directly compared and where sexual killers were male, predominantly non-serial and perpetrated against adult female victims were included. Results indicate that levels of loneliness and anger appear to be more prevalent in the sexual killer sample while mental health disorders, criminal history, family structure and history of sexual abuse as well as some crime scene behaviours seem to be similar across the two groups. The results of this review have also highlighted some methodological problems inherent to these types of studies and these issues are discussed from the perspective of improving research on these crimes

Subacute ruminal acidosis affects fermentation and endotoxin concentration in the rumen and relative expression of the CD14/TLR4/MD2 genes involved in lipopolysaccharide systemic immune response in dairy cows

The first objective of this study was to investigate the effects of subacute ruminal acidosis (SARA) on fermentation, ruminal free lipopolysaccharides (LPS), and expression of the cluster of differentiation 14 (CD14), toll-like receptor 4 (TLR4), and myeloid differentiation protein 2 (MD2) complex in white blood cells involved in the systemic immune response in dairy cows. The second objective was a study of whether increased expression of the LPS receptor complex led to increases in the concentrations of plasma high-density lipoprotein (HDL) and serum Ca. Three hundred five dairy cows located in 13 Polish high-yielding dairy commercial farms were selected according to their days in milk (40-150 d; average = 75), 305-d milk yield (10,070-12,041 kg; average = 10,940), and number of lactations (primiparous, n = 139 and multiparous, n = 166). Next, the herds were segregated into 3 groups based on the percentages of cows with an assigned value of ruminal fluid pH: SARA-positive, SARA-risk, and SARA-negative herds. Moreover, 305 selected dairy cows were divided according to the classification based on ruminal fluid pH into 3 groups as healthy (pH > 5.81), risk (pH 5.8-5.6) and acidotic cows (pH < 5.6). Rumen fluid samples were collected via rumenocentesis. In the AC group, we recorded higher concentrations of ruminal free LPS [4.57 Log(10) endotoxin units (EU)/mL; 42,206 EU/mL] compared with the healthy group (4.48 Log(10) EU/mL; 34,179 EU/mL). Similarly, the concentration of ruminal free LPS was higher in SARA-positive herds (4.60 Log(10) EU/mL; 43,000 EU/mL) compared with SARA-negative herds (4.47 Log(10) EU/mL; 32,225 EU/mL). The relative mRNA abundance of genes associated with the function of LPS receptors, such as CD14, TLR4, and MD2, in white blood cells differed between all experimental groups on both cow and herd levels. In the acidotic group, we recorded higher concentrations of HDL (78.16 vs. 68.32 mg/dL) and serum amyloid A (10.80 vs. 9.16 mu g/mL) and lower concentrations of Ca (8.26 vs. 10.16 mg/dL) and haptoglobin (470.19 vs. 516.85 ng/mL) compared with the healthy group. Similar results were obtained in the SARA herd status analysis, but the concentration of lipopolysaccharide-binding protein differed statistically. Moreover, the pH of ruminal fluid was negatively correlated with relative mRNA abundance of genes such as CD14, TLR4, MD2, and concentrations of serum HDL and serum amyloid A, although positively correlated with serum Ca. The results indicated that decreases in ruminal fluid pH increased the release of free LPS into the rumen and stimulated the expression of the LPS receptor complex and immune response. Moreover, an increase in the expression of the LPS receptor led to higher concentrations of plasma HDL and lower serum Ca, which may be a protective mechanism against endotoxemia. However, the biological significance of these results needs to be investigated further in larger field trials

“We boil at different degrees”: factors associated with severity of attack in sexual killing

Degree of injury, as measured by the Homicide Injury Scale (HIS), was examined to advance understanding of the dynamics of sexual killing. A total of 350 nonserial, male sexual killers were included, and the different ways that the sexual element of their offenses and the act of killing were connected was accounted for by determining that cases were either directly sexual (the sexual element and killing were closely bound), or indirectly sexual (killing was not a source of sexual stimulation). The two groups, direct and indirect sexual killers, were each subjected to multiple linear regression analyses to examine the group-specific relationship between level of injury and predictor variables previously found to be associated with increased severity of attack. No differences in the mean total HIS scores between the indirect and the direct cases were found, suggesting a comparable emotional intensity between the groups. However, given that the groups differed in terms of the functional role of fatal violence, severity of attack could not be sufficiently explained as driven by anger. In line with this hypothesis, different predictors appeared to be associated with increased degree of injury sustained by victims of indirect compared with direct sexual killers. As such, situational components appear to play a role in the behavior of indirect sexual killers, whereas the behavior of direct perpetrators tends to be linked with the enactment of existing deviant fantasies. The role of anger in sexual homicide is discussed further, and overall, it is argued that irrespective of whether violence was initially driven by anger, evidence of sexual arousal to severe violence must be scrutinized within sexual homicide research as well as in psycholegal contexts

The use of crime scene and demographic information in the identification of non-serial sexual homicide

As with other sexual offenders, sexual homicide perpetrators can be reluctant to talk about their criminal behavior. Therefore, in homicide cases, forensic practitioners frequently rely on crime scene information to identify any sexual behavior associated with the offense. This study aims to identify objective and readily available crime scene information, alongside information about victims and perpetrators, based on 65 cases from England and Wales in the United Kingdom of men convicted of homicide who had committed a non-serial sexual homicide and 64 cases of men convicted of homicide where the available evidence indicated that it was a non-serial non-sexual homicide. Chi-square tests and logistic regression were used to analyze the data. There were few differences in terms of demographic information and criminal histories between the two perpetrator groups. There were crime scene indicators supporting the use of Ressler et al.’s definition of sexual homicide. The victims of sexual homicide were generally found in their home with the lower half of the body exposed and with evidence of vaginal sex. Furthermore, extreme injuries and strangulation were more frequent in sexual homicides. Use of weapon was associated with a non-sexual homicide. Victims of sexual homicide were as likely to know the perpetrator as not. Potential benefits of the characteristics reported to investigators and forensic practitioners tasked with identifying sexual homicides are discussed and areas for further research suggested