Two-choice oviposition assays.

<p>Mean number (± SEM) of eggs laid by two-day old gravid female <i>L</i>. <i>ingenua</i> flies on various pure fungal cultures grown on potato dextrose agar in two-choice oviposition assays. Each horizontal bar shows the mean number of eggs laid on the two choices of fungi by 15 two-day-old gravid female flies, tested in groups of three in three individual chambers with 5 different cohorts of females being tested over 5 different nights. All data were non-normally distributed and differences between the mean number of eggs deposited on each pair of fungal cultures were analyzed via the Mann–Whitney <i>U</i> test (two-tailed, df = 14).</p

Larval survival assays.

<p>Mean survival (± SEM) of <i>Lycoriella ingenua</i> larvae to adults on various pure fungal cultures grown on potato dextrose agar. Each histogram is the mean survival of 5 newly emerged first instar larvae, replicated from 6 different cohorts of larvae. All data were non-normally distributed and differences among mean survival on different fungal cultures were first analyzed via the Kruskal-Wallis test. Differences between survival on different fungal cultures were compared with the Dunn’s Multiple Comparisons Test. Different letters above bars indicate a significant difference (df = 5; P < 0.05).</p

No-choice oviposition assays.

<p>Mean number (± SEM) of eggs laid by 0- (day of emergence) to-7 day-old female <i>L</i>. <i>ingenua</i> flies on various pure fungal cultures grown on potato dextrose agar in no-choice oviposition assays. A total of 15 flies of a particular age were tested for their tendency to lay eggs on each of the 8 fungal cultures under no-choice conditions, resulting in a total of 120 females (15 flies x 8 fungal cultures) of each age that were tested for oviposition on all fungal cultures. There were 8 age groups tested (panels a-h), and thus 840 different female flies were used in this experiment. All data were non-normally distributed and differences among mean egg numbers within each age group were analyzed via the Kruskal-Wallis test. Differences between mean egg numbers for each age group were compared using the Dunn’s Multiple Comparisons Test. No comparisons were made between mean numbers of eggs in different age groups. Different letters above histograms within the same age group indicate a significant difference (df = 14; P < 0.05).</p

Two-choice attraction assays.

<p>Mean number (± SEM) of two-day-old gravid female <i>Lycoriella ingenua</i> flies attracted to various pure fungal cultures grown on potato dextrose agar in two-choice, static-flow olfactometer assays. Each horizontal bar is the mean of responses of 5 females to each treatment over 12 replicates (N = 12). The mean number of non-responders (± SEM) for each combination is included in parentheses to the right. Female choices for each pair of cultures were analyzed via chi square. All non-responders were excluded from the analysis.</p