84 research outputs found
An hypothesis about the reproductive modes of Phaeoacremonium aleophilum and Phaeomoniella chlamydospora.
Isolation and characterization of non cerato-ulmin producing laboratory induced mutants of Ophiostoma novo-ulmi.
A Hypotesis about the Reproductive Modes of «Phaeoacremonium aleophilum» and «Phaeomoniella chlamydospora»
The mode of reproduction of Phaeomoniella chlamydospora (syn.: Phaeoacremonium chlamydosporum)
(Pch) and Phaeoacremonium aleophilum (Pal), two mitosporic fungi involved in producing symptoms of esca and a
decline of young grapevines, was studied by analysing the amplification profiles obtained in RAPD- and RAMS-PCR
experiments (RAPD= Random Amplified Polymorphic DNA; RAMS= Random Amplified Micro- or Mini-Satellites). A
low level of polymorphism was found in both species, but no relationship was detected between the genetic variation
of isolates and their geographic origin. However, greater variation was found in Pal than in Pch isolates, and more
with the RAPD than with the RAMS experiments, as shown by the percentage of polymorphic fragments and multilocus
genotypes obtained. The clonal fraction of the Pal and Pch populations, as well as the gametic disequilibrium tests
suggested the existence of sexual reproduction in both these species; nevertheless the contribution of asexual reproduction
to the genetic structure of the natural populations of Pch seemed to be greater than that of sexual reproduction.
With Pal the opposite is thought to be the case
Naturally occurring non cerato-ulmin producing mutants of Ophiostoma novo-ulmi are pathogenic but lack aerial mycelium
Sequence analysis of ITS ribosomal DNA in five Phaeoacremonium species and development of a PCR-based assay for the detection of P. chlamydosporum and P. aleophilum in grapevine tissue.
Variations among Italian isolates of the Heterobasidion annosum P Group detected using the M13 minisatellite.
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