1 research outputs found
Identification of Metabolic Pathways in <i>Daphnia magna</i> Explaining Hormetic Effects of Selective Serotonin Reuptake Inhibitors and 4‑Nonylphenol Using Transcriptomic and Phenotypic Responses
The molecular mechanisms explaining
hormetic effects of selective
serotonin reuptake inhibitors (SSRIs) and 4-nonylphenol in <i>Daphnia magna</i> reproduction were studied in juveniles and
adults. Transcriptome analyses showed changes in mRNA levels for 1796
genes in juveniles and 1214 genes in adults (out of 15 000
total probes) exposed to two SSRIs (fluoxetine and fluvoxamine) or
to 4-nonylphenol. Functional annotation of affected genes was improved
by assuming the annotations of putatively homologous <i>Drosophila</i> genes. Self-organizing map analysis and partial least-square regression
coupled with selectivity ratio procedures analyses allowed to define
groups of genes with specific responses to the different treatments.
Differentially expressed genes were analyzed for functional enrichment
using Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes databases.
Serotonin metabolism, neuronal developmental processes, and carbohydrates
and lipid metabolism functional categories appeared as selectively
affected by SSRI treatment, whereas 4-nonylphenol deregulated genes
from the carbohydrate metabolism and the ecdysone regulatory pathway.
These changes in functional and metabolic pathways are consistent
with previously reported SSRIs and 4-nonylphenol hormetic effects
in <i>D. magna</i>, including a decrease in reserve carbohydrates
and an increase in respiratory metabolism