Correlation between miRNA plasma expression at timepoint V3 and plasma levels of troponin T at timepoint V3.

<p>A miR-1, B miR-133a, C miR-30a, D miR-26a, E miR-29b. * p< 0.05, ** p<0.01, *** p<0.001 Spearman correlation coefficient.</p

Correlation between miRNA plasma expression and Absorbance at 414 nm.

<p>Different time points (V1-V4) shown from top to bottom, different miRNAs (miR-1, -26a, -29b, -30a, -133a) are shown from left to right. Spearman correlation coefficient.</p

MicroRNA plasma expression over time.

<p>A miR-1, B miR-133a, C miR-30a, D miR-26a, E miR-29b. V1 baseline, V2 after a 10 week training period, V3 immediately after the marathon, V4 24 hours after the marathon. Data shown as MEAN±SEM, * p < 0.05, ** p<0.01, *** p<0.001 vs. timepoint V1, # p < 0.05, ## p<0.01, ### p<0.001 vs. timepoint V2, † p < 0.05, †† p<0.01, ††† p<0.001 vs. timepoint V4, Friedman-Test.</p

Correlation between miRNA plasma expression at timepoint V3 and LA diameter at timepoint V4.

<p>A miR-1, B miR-133a, C miR-30a, D miR-26a, E miR-29b. * p< 0.05, ** p<0.01, *** p<0.001 Spearman correlation coefficient.</p

Impact of delayed processing on separated blood fractions.

<p>Whole blood was collected into EDTA or serum separator containing tubes and incubated at room temperature for 0h, 24h, and 4 days before processed into plasma (A) and serum (B) or processed into plasma (C) and serum (D) immediately and then incubated at room temperature for 0h, 24h, and 4d. Data presented as normalized average ΔC_Tvalues±SEM. *p<0.05, **p<0.01, ***p<0.001.</p

Stability of miRNA in whole blood incubated at room temperature.

<p>Whole blood was collected into EDTA containing tubes and incubated for 0, 4, 8, 12, 24 and 72 h at room temperature before processed into plasma. qRT-PCR was performed. Statistically significant differences in miRNA expression are marked with asterisks (**). **p<0.01. A) miR-21. B) miR-29b.</p

MiRNA stability after repetitive freeze-thaw cycles within the separated fractions.

<p>Whole blood was collected into EDTA or serum separator containing tubes, immediately processed into plasma (A) and serum (B) and frozen at -80°C. Samples were thawed one or four times before RNA was isolated and RT-qPCR was performed. *p<0.05, **p<0.01, unpaired student t-test.</p

Incubation of plasma at -80°C for up to 9 months.

<p>There was no significant change in miRNA concentration up to 9 months after freezing for either miR-21 (A) or miR-29b (B) in plasma (p = 0.11 and p = 0.96). However, the concentration of both miRNAs in whole blood increased during storage at -80°C. *p<0.05, **p<0.01.</p

MiRNA levels in the 3 blood fractions; plasma, buffy coat and red blood cells (RBC).

<p>The two miRNAs, miR-21 and miR-29b, were differently distributed in the fractions; there are significant higher levels of miR-21 in the buffy coat and RBC compared to plasma, and miR-29b plasma levels are significantly lower than in the buffy coat. Statistically significant differences in miRNA expression are marked with asterisks (*).**p<0.01, ***p<0.001.</p

Illustration of alarm subtypes.

The pie chart illustrates the distribution of alarm subtypes generated by ICM remote monitoring. In total, 29,180 episodes were assessed.</p