CD8+ T-cells infiltrating tumor tissue.

<p>Microscope: Carl Zeiss, Jena, Germany, 20-fold magnification, room temperature, Adobe Photoshop.</p

Log-Rank-Test FoxP3+.

<p>Peripheral accumulation of FoxP3+ Cells leads to reduced survival (p = 0.056).</p

Innate immune activation by <i>Mycoplasma hyorhinis</i>-infected B16 cells is mediated by a protein factor.

<p>Bone marrow cells were cultured in the presence of cell lysates of <i>Mycoplasma hyorhinis</i>-infected B16 cells. Some cell lysates were pre-treated either with DNase, RNase or proteinase K. 18 h later, IL-6 levels in the bone marrow cell culture supernatant were analyzed by ELISA. Protein digestion was performed at 55°C. In one condition, B16 cell lysates were heated to 55°C in the absence of proteinase K. Data give the mean values of triplicate samples + S.E.M. and are representative of two independent experiments. Asterisks indicate statistically significant difference to the appropriate non-inhibitor-treated conditions. ND, not determined.</p

Antibodys used in this study.

<p>Antibodys used in this study.</p

Plasmacytoid dendritic cells produce IFN-α and IL-6 in response to <i>Mycoplasma hyorhinis</i>-infected B16 cells.

<p>Bone marrow-derived plasmacytoid DCs were cultured in the presence of decreasing concentrations of lysates derived from <i>Mycoplasma hyorhinis</i>-infected B16 cells <b>(A, C)</b> or different TLR stimuli <b>(B, D)</b>. In some conditions, chloroquine was added to the pDC cultures. After 18 h, IL-6 and IFN-α levels in the pDC culture supernatant were analyzed by ELISA. Data give the mean + S.E.M. of triplicate samples and are representative of at least two independent experiments. Asterisks indicate statistically significant differences to the appropriate wild-type or untreated conditions.</p

Patient and tumor characteristics.

<p>Patient and tumor characteristics.</p

Amount of cells.

<p>Amount of cells.</p

Scoring System.

<p>Scoring System.</p

CD8+ T-cells: Location.

<p>CD8+ T-cells: Location.</p

Tumor infiltration of cells.

<p>Tumor infiltration of cells.</p