64 research outputs found
Observation of superoxide production during catalysis of Bacillus subtilis oxalate decarboxylase at pH 4
This contribution describes the trapping of the hydroperoxyl radical at a pH of 4 during turnover of wild-type oxalate decarboxylase and its T165V mutant using the spin trap BMPO. Radicals were detected and identified by a combination of EPR and mass spectrometry. Superoxide, or its conjugate acid, the hydroperoxyl radical, is expected as an intermediate in the decarboxylation and oxidation reactions of the oxalate monoanion both of which are promoted by oxalate decarboxylase. Another intermediate, the carbon dioxide radical anion was also observed. The quantitative yields of superoxide trapping is similar in the wild type and the mutant while it is significantly different for the trapping of the carbon dioxide radical anion. This suggests that the two radicals are released from different sites of the protein
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Test procedure to evaluate scavenging of TBP plant solvent extraction waste with nickel ferrocyanide
This memorandum has been prepared to define the proposed plant waste scavenging test and to present an operating flow diagram and operating procedures for use during the test
RECURRENT CALCIUM OXALATE UROLITHIASIS IN A MAN WITH FAMILIAL PERIODIC PARALYSIS AND HYPOKALEMIA
RECURRENT CALCIUM OXALATE UROLITHIASIS IN A MAN WITH FAMILIAL PERIODIC PARALYSIS AND HYPOKALEMIA
Insights into the fragmentation pathways of gas-phase protonated sulfoserine
The fragmentation chemistry of protonated sulfoserine was probed using a combination of collision-induced dissociation (CID) mass spectrometry, infrared multiple photon dissociation (IRMPD) spectroscopy, and density functional theory (DFT) calculations. The IRMPD spectra of the dominant fragment ions at m/z 106 and 88 (Le., loss of SO3 and H2SO4) were obtained and used to determine the corresponding structures. By comparison to a synthetic standard and calculations, it was determined that the m/z 106 ion is structurally identical to protonated serine. The m/z 88 fragment ion was assigned an aziridine structure based on a comparison to theory, analogous to the structure previously proposed by others for phosphoric acid loss from phosphoserine. This work provides the first spectroscopic insights into the dissociation pathways of a sulfated amino acid, laying the groundwork for future studies on related amino acids and peptides with this important, labile post-translational modification
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