19 research outputs found
Gallbladder microbiota in healthy dogs and dogs with mucocele formation
To date studies have not investigated the culture-independent microbiome of bile from dogs, a species where aseptic collection of bile under ultrasound guidance is somewhat routine. Despite frequent collection of bile for culture-based diagnosis of bacterial cholecystitis, it is unknown whether bile from healthy dogs harbors uncultivable bacteria or a core microbiota. The answer to this question is critical to understanding the pathogenesis of biliary infection and as a baseline to exploration of other biliary diseases in dogs where uncultivable bacteria could play a pathogenic role. A pressing example of such a disease would be gallbladder mucocele formation in dogs. This prevalent and deadly condition is characterized by excessive secretion of abnormal mucus by the gallbladder epithelium that can eventually lead to rupture of the gallbladder or obstruction of bile flow. The cause of mucocele formation is unknown as is whether uncultivable, and therefore unrecognized, bacteria play any systematic role in pathogenesis. In this study we applied next-generation 16S rRNA gene sequencing to identify the culture-negative bacterial community of gallbladder bile from healthy dogs and gallbladder mucus from dogs with mucocele formation. Integral to our study was the use of 2 separate DNA isolations on each sample using different extraction methods and sequencing of negative control samples enabling recognition and curation of contaminating sequences. Microbiota findings were validated by simultaneous culture-based identification, cytological examination of bile, and fluorescence in-situ hybridization (FISH) performed on gallbladder mucosa. Using culture-dependent, cytological, FISH, and 16S rRNA sequencing approaches, results of our study do not support existence of a core microbiome in the bile of healthy dogs or gallbladder mucus from dogs with mucocele formation. Our findings further document how contaminating sequences can significantly contribute to the results of sequencing analysis when performed on samples with low bacterial biomass
Two-species percolation and Scaling theory of the metal-insulator transition in two dimensions
Recently, a simple non-interacting-electron model, combining local quantum
tunneling via quantum point contacts and global classical percolation, has been
introduced in order to describe the observed ``metal-insulator transition'' in
two dimensions [1]. Here, based upon that model, a two-species-percolation
scaling theory is introduced and compared to the experimental data. The two
species in this model are, on one hand, the ``metallic'' point contacts, whose
critical energy lies below the Fermi energy, and on the other hand, the
insulating quantum point contacts. It is shown that many features of the
experiments, such as the exponential dependence of the resistance on
temperature on the metallic side, the linear dependence of the exponent on
density, the scale of the critical resistance, the quenching of the
metallic phase by a parallel magnetic field and the non-monotonic dependence of
the critical density on a perpendicular magnetic field, can be naturally
explained by the model.
Moreover, details such as the nonmonotonic dependence of the resistance on
temperature or the inflection point of the resistance vs. parallel magnetic are
also a natural consequence of the theory. The calculated parallel field
dependence of the critical density agrees excellently with experiments, and is
used to deduce an experimental value of the confining energy in the vertical
direction. It is also shown that the resistance on the ``metallic'' side can
decrease with decreasing temperature by an arbitrary factor in the degenerate
regime ().Comment: 8 pages, 8 figure
On the Protein Crystal Formation as an Interface-Controlled Process with Prototype Ion-Channeling Effect
A superdiffusive random-walk action in the depletion zone around a growing protein crystal is considered. It stands for a dynamic boundary condition of the growth process and competes steadily with a quasistatic, curvature-involving (thermodynamic) free boundary condition, both of them contributing to interpret the (mainly late-stage) growth process in terms of a prototype ion-channeling effect. An overall diffusion function contains quantitative signatures of both boundary conditions mentioned and indicates whether the new phase grows as an orderly phase or a converse scenario occurs. This situation can be treated in a quite versatile way both numerically and analytically, within a generalized Smoluchowski framework. This study can help in (1) elucidating some dynamic puzzles of a complex crystal formation vs biomolecular aggregation, also those concerning ion-channel formation, and (2) seeing how ion-channel-type dynamics of non-Markovian nature may set properly the pace of model (dis)ordered protein aggregation