Predictive scales and ECG indicators in the acute stroke period

The features of the ECG in patients with different values of the SAPS and APACHE II scales in the acute period of stroke were investigated. Despite the fact that the APACHE II and SAPS scales do not include ECG parameters, a number of ECG parameters significantly differ in patients with acute stroke at different values according to these scales. The prognosis aggravation according to the studied scales was not always accompanied by the same type of ECG parameters changes. Survived patients with high APACHE II scores were characterized by high dysmorphic values of ECG amplitude parameters. Died patients with high risks on both scales showed an increase in dysmorphism in many ECG parameters. The scientific novelty of the study is in the detection of ECG features at different values of the SAPS and APACHE II scales in the acute period of stroke.Изучены особенности ЭКГ у пациентов при разных значениях шкал SAPS и APACHE II в остром периоде инсульта. Несмотря на то, что шкалы APACHE II и SAPS не включают в себя параметры ЭКГ, у пациентов с острым инсультом при различных значениях по данным шкалам ряд показателей ЭКГ достоверно отличались. Ухудшение прогноза по изученным шкалам не всегда сопровождалось однотипными изменениями показателей ЭКГ. Выжившие пациенты с высокими значениями по шкале APACHE II характеризовались большими показателями дизморфности амплитудных значений ЭКГ. Умершие пациенты с высокими рисками по обеим шкалам демонстрировали увеличение дизморфности по многим показателям ЭКГ. Научная новизна исследования заключается в обнаружении особенностей ЭКГ при различных рисках осложнений по шкалам SAPS и APACHE II в остром периоде инсульта

STATUS OF ITEP DECABORANE ION SOURCE PROGRAM.

The joint research and development program is continued to develop steady-state ion source of decaborane beam for ion implantation industry. Both Freeman and Bemas ion sources for decaborane ion beam generation were investigated. Decaborane negative ion beam as well as positive ion beam were generated and delivered to the output of mass separator. Experimental results obtained in ITEP are presented

G-quadruplex structures mark human regulatory chromatin

G-quadruplex (G4) structural motifs have been linked to transcription, replication and genome instability and are implicated in cancer and other diseases. However, it is crucial to demonstrate the bona fide formation of G4 structures within an endogenous chromatin context. Herein we address this through the development of G4 ChIP-seq, an antibody-based G4 chromatin immunoprecipitation and high-throughput sequencing approach. We find ∼10,000 G4 structures in human chromatin, predominantly in regulatory, nucleosome-depleted regions. G4 structures are enriched in the promoters and 5' UTRs of highly transcribed genes, particularly in genes related to cancer and in somatic copy number amplifications, such as $\textit{MYC}$. Strikingly, $\textit{de novo}$ and enhanced G4 formation are associated with increased transcriptional activity, as shown by HDAC inhibitor-induced chromatin relaxation and observed in immortalized as compared to normal cellular states. Our findings show that regulatory, nucleosome-depleted chromatin and elevated transcription shape the endogenous human G4 DNA landscape.European Molecular Biology Organization (EMBO Long-Term Fellowship), University of Cambridge, Cancer Research UK (Grant ID: C14303/A17197), Wellcome Trust (Grant ID: 099232/z/12/z

Validation of the anabolic steroids determination method in biological active additives for sportsmen

The validation of the method of qualitative determination of anabolic steroids in biological active additives for sportsmen by gas chromatography with mass-detection has been carried out in this work. The experimental researches have been carried out at the gas chromatograph Agilent 7890 А and mass-spectrometer Agilent 7000 with triple quadrupole at health protection institution “National anti-doping laboratory”. The sample preparation has been done by the extraction of the analyzed compounds and their tr ansformation into volatile trimethylsilyl derivatives. The identification of these received compounds has been carried out by their reten-tion time and their mass-spectra. The de termination of validation characteristics of qualitative determination of some anabolic steroids in a biological active additive for sportsmen “Isolife isotonic” has been carried out: 17-α-metyltestosterone, dihydroepiandr osterone, nandrolone, methandienone, testosterone. As a result of validation of this method it has been confirmed that such characteristics as selectivity, limit of detection, sample carrying, robustness are in the accordance with acceptable criteria. Сonsequently, this method could be used for finding out anabolic steroids in biolog ical active additives and special food for sportsmen

Distinct Properties of Human HMGN5 Reveal a Rapidly Evolving but Functionally Conserved Nucleosome Binding Protein ▿

The HMGN family is a family of nucleosome-binding architectural proteins that affect the structure and function of chromatin in vertebrates. We report that the HMGN5 variant, encoded by a gene located on chromosome X, is a rapidly evolving protein with an acidic C-terminal domain that differs among vertebrate species. We found that the intranuclear organization and nucleosome interactions of human HMGN5 are distinct from those of mouse HMGN5 and that the C-terminal region of the protein is the main determinant of the chromatin interaction properties. Despite their apparent differences, both mouse and human HMGN5 proteins interact with histone H1, reduce its chromatin residence time, and can induce large-scale chromatin decompaction in living cells. Analysis of HMGN5 mutants suggests that distinct domains in HMGN5 affect specific steps in the interaction of H1 with chromatin. Elevated levels of either human or mouse HMGN5 affect the transcription of numerous genes, most in a variant-specific manner. Our study identifies HMGN5 as a rapidly evolving vertebrate nuclear protein with species-specific properties. HMGN5 has a highly disordered structure, binds dynamically to nucleosome core particles, modulates the binding of H1 to chromatin, reduces the compaction of the chromatin fiber, and affects transcription