Neuroendocrine and Behavioral Responses and Brain Pattern of c- fos Induction Associated with Audiogenic Stress

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72579/1/j.1365-2826.1997.00593.x.pd

Technology Transfer in the People\u27s Republic of China: An Assessment

Perhaps the most striking aspect of the trading relationship between the United States and the People\u27s Republic of China is its explosive growth over the last fifteen years. In 1973, the total value of bilateral trade between the U.S. and China was $805 million (up from a mere$5 million just two years earlier). In 1987, this figure reached $10.4 billion - an increase of over 1000 per cent. This growth notwithstanding, the United States is not a commanding presence in the PRC\u27s overall trade picture. The U.S. share of total PRC imports in 1986 (almost$43 billion) is only 7 .2 percent, and the PRC accounted for only slightly over 1 percent of U.S. imports

Glucocorticoid Regulation of Stress-Induced Mineralocorticoid Receptor Gene Transcription in Vivo

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73787/1/j.1749-6632.1994.tb39292.x.pd

Analysing co-evolution among artificial 3D creatures

This paper is concerned with the analysis of coevolutionary dynamics among 3D artificial creatures, similar to those introduced by Sims (1). Coevolution is subject to complex dynamics which are notoriously difficult to analyse. We introduce an improved analysis method based on Master Tournament matrices [2], which we argue is both less costly to compute and more informative than the original method. Based on visible features of the resulting graphs, we can identify particular trends and incidents in the dynamics of coevolution and look for their causes. Finally, considering that coevolutionary progress is not necessarily identical to global overall progress, we extend this analysis by cross-validating individuals from different evolutionary runs, which we argue is more appropriate than single-record analysis method for evaluating the global performance of individuals

Enkephalin systems in diencephalon and brainstem of the rat

The immunocytochemical distribution of [Leu]enkephalin and an adrenal enkephalin precursor fragment (BAM-22P) immunoreactivity was investigated in the diencephalon and brainstem of rats pretreated with relatively high doses of colchicine (300–400 Μg/10 Μl intracerebroventricularly). The higher ranges of colchicine pretreatment allowed the visualization of extensive enkephalin-containing systems in these brain regions, some of which are reported for the first time. Immunoreactive perikarya were found in many hypothalamic and thalamic nuclei, interpeduncular nucleus, substan-tia nigra, the colliculi, periaqueductal gray, parabrachial nuclei, trigeminal motor and spinal nuclei, nucleus raphe magnus and other raphe nuclei, nucleus reticularis paragigantocellularis, vestibular nuclei, several nor-adrenergic cell groups, nucleus tractus solitarius, as well as in the spinal cord dorsal horn. In addition to the above regions, immunoreactive fibers were also noted in the habenular nuclei, trigeminal sensory nuclei, locus coeruleus, motor facial nucleus, cochlear nuclei, dorsal motor nucleus of the vagus, and hypoglossal nucleus. When adjacent sections to those stained for [Leu]enkephalin were processed for BAM-22P immunoreactivity, it was found that these two immunoreactivities were distributed identically at almost all anatomical locations. B AM-22P immunoreactivity was generally less pronounced and was preferentially localized to neuronal perikarya. The results of the present as well as the preceding studies (Khachaturian et ai., '83) strongly suggest substantial structural similarity between the adrenal proenkephalin precursor and that which occurs in the brain. Also discussed are some differences and parallels between the distribution of [Leu]enkeph-alin and dynorphin immunoreactivities.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/50020/1/902200305_ftp.pd

An adjudicated hermeneutic single-case efficacy design study of experiential therapy for panic/phobia

This paper illustrates the application of an adjudicated form of Hermeneutic Single Case Efficacy Design (HSCED), a critical-reflective method for inferring change and therapeutic influence in single therapy cases. The client was a 61 year-old European-American male diagnosed with panic and bridge phobia. He was seen for 23 sessions of individual Process-Experiential/Emotion-Focused Therapy. In this study, affirmative and skeptic teams of researchers developed opposing arguments regarding whether the client changed over therapy and whether therapy was responsible for these changes. Three judges representing different theoretical orientations then assessed data and arguments, rendering judgments in favor of the affirmative side. We discuss clinical implications and recommendations for the future interpretive case study research

Stress systems in the brain: molecules, nuclei and circuits

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/31355/1/0000266.pd

A Comparison of Boosted-Discharge Hollow Cathode Lamps and an Inductively Coupled Plasma (ICP) as Excitation Sources in ICP Atomic Fluorescence Spectrometry

Copper, nickel and lead boosted-discharge hollow cathode lamps, run at recommended currents, have been compared with a high-powered inductively coupled plasma (ICP) as excitation sources in atomic fluorescence spectrometry (AFS). A similar comparison was made with a copper lamp run at higher currents. It was found that for lead and nickel, the fluorescence spectra differed in the relative intensities of the transitions observed with the two sources. No evidence was found for a difference in radiances between the two sources when the lamp was overrun. Although the lamps gave rise to lower blank standard deviation values, detection limits were worse because of poorer sensitivity due to the inability of the circular source to illuminate the required atom cell volume in the atomiser. It was concluded that the ICP was the better source, when the criterion is detection limits, but the lamps may be more convenient in some circumstances

Changes in Proopiomelanocortin Primary Transcript Levels in the Anterior Pituitary Accompany Increased Adrenocorticotropin Secretion During the Diurnal Surge

Proopiomelanocortin (POMC) gene transcription in the anterior pituitary varies during stress and glucocorticoid feedback. These changes appear to parallel alterations in peptide release. The diurnal rhythm of the hypothalamo-pituitary-adrenal axis also involves the periodic excursion of adrenocorticotropin (ACTH) levels in plasma, but it is not clear whether the diurnal release is accompanied by changes at the transcriptional level. In the present study, we have initially characterized the heteronuclear species of POMC (hnPOMC) RNA found in the anterior pituitary by a Northern blot analysis and subsequently used this method to quantitate relative changes in the levels of heteronuclear transcript during diurnal stimulation. Two species of RNA migrating at 6.0 kb and 4.1 kb were found in the nuclear fraction of the anterior pituitary. Successive probing by various POMC cRNAs indicated that the 6.0 kb fragment was the primary transcript and the 4.1 kb fragment corresponded to the intron A-containing processing intermediate of POMC. The nuclear species were quantitated after acute swim stress and during the diurnal ACTH secretion. Acute swim increased plasma ACTH levels by 243% after 30 min. This was paralleled by a 214% increase in the primary transcript RNA levels. Endogenous circadian stimulation in the evening produced a smaller rise of plasma ACTH (79%), and was accompanied by a 34% increase in POMC hnRNA levels. Nuclear processing intermediate (4.1 kb) and the mRNA levels did not vary during the evening. These results suggest that the diurnal mechanism transiently increases ACTH release as well as POMC gene transcription in the anterior pituitary. Release and transcription appear to be tightly coupled during circadian activation as well as during stress.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75121/1/j.1365-2826.1992.tb00340.x.pd