Plasma corticosterone (CORT) levels.

<p>(A) Basal CORT (ng/ml). (B) ND-groups. (C) LgA-groups. Data are expressed as mean±SEM, **p<0.01; *p<0.05.</p

Corticosteroid receptors gene expression in DG.

<p>(A) GR mRNA. γp<0.05 vs. ND-Day 1; *p<0.05 vs. LgA-Day 1. (B) GR mRNA by anatomical level at Day 28 of cocaine withdrawal. **p<0.01. (C) MR mRNA. (D) MR mRNA by anatomical level at Day 28 of cocaine withdrawal. *p<0.05. (E) MR/GR mRNA ratio. **p<0.01. (F) Representative X-ray image of GR and MR mRNA in anterior (−3.60 mm from Bregma) and posterior DG (−5.30 mm from Bregma). Data are expressed as mean±SEM.</p

Escalation of cocaine intake during LgA cocaine SA procedures.

<p>Data represent mean±SEM amount of cocaine intake (0.5 mg/kg cocaine per infusion) over the entire daily sessions (5-h session per day of cocaine SA for 14 sessions) for session day 1 vs. day 14, ***p<0.001.</p

Stress-related gene expression in DG.

<p>(A) HSP90AA1 mRNA. (B) HSP90AA1 mRNA by anatomical level at Day 28 of cocaine withdrawal. *p<0.05; **p<0.01. (C) Representative X-ray image of HSP90AA1 mRNA in anterior and posterior DG. (D) HSP90AB1 mRNA. (E) HSP90AB1 mRNA by anatomical level at Day 28 of cocaine withdrawal. (F) Representative X-ray image of HSP90AB1 mRNA in anterior and posterior DG. Data are expressed as mean±SEM. Anterior (−3.60 mm from Bregma) and posterior (−5.30 mm from Bregma) DG.</p

GR, MR, MR/GR, HSP90AA1 and HSP90AB1 mRNA in CA subfields of the hippocampus.

<p>Data are expressed as mean±SEM mRNA level (corrected IOD). *p<0.05 when comparing LgA-Day 1 vs. LgA-Day 28.</p

Prohormone hierarchical grammar of evolutionary MPHMM modules.

<p>Prohormone hierarchical grammar of evolutionary MPHMM modules.</p

Modules and their abbreviations.

<p>Modules and their abbreviations.</p

Matches Found in Swiss-Prot Database.

<p><b>False Positives</b></p><p><u>Other signaling molecules</u>: FGF-3,5,7,10,17,18; GDNF; CD8,28; PDGF-2; TGF; VEGF (vascular endothelial growth factor); HBNF-1; MIP; NGF (nerve growth factor); Cytokine A21, IFN-α (interferon alpha); IGF binding protein 1B,2,3; IL7 (interleukin 7).</p><p><u>Other</u>: MAGF (microfibril associated protein), MINK (K-channel), K-channel related peptide, L-type Ca²⁺ channel, gamma subunit, myelin Po protein, Dif-2, Eosinophil, Syntaxin 1B (vesicle docking), Syntaxin 2, TMP21 (vesicle trafficking protein), Coagulation factor III, PGD2 synthase, syndecans, FKBP12 (FK506 binding protein), Folate receptor, ERp29, COMT, Connexin 32, Cytostatin.</p

In situ hybridization of preproNPQ mRNA.

<p>Expression of preproNPQ mRNA at the level of the caudal ventrolateral periaqueductal gray (PAG). ISH autoradiograms were digitized and pseudocolored according to the same scheme as in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1000258#pcbi-1000258-g007" target="_blank">Figure 7</a>. NPQ signal was visible in the ventrolateral quadrant of the PAG as well as within the underlying reticular formation (A, D, G). ISHs for TH (B), ChAT (E) and TPH2 (H) were carried out on adjacent sections. Arrow in panel B indicates location of dopaminergic TH-positive neurons of the ventrolateral PAG that appear to overlap with a subset of NPQ mRNA (C). There is also close spatial relationship between NPQ and ChAT (F) and NPQ and TPH2 (I). Abbreviations are the same as in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1000258#pcbi-1000258-g007" target="_blank">Figure 7</a>.</p

Northern Blot Analysis of preproNPQ mRNA.

<p>Ambion's First Choice Human Blot was prehybridized and probed with human NPQ cDNA prepared from the human DNA clone in pOTB7 vector from ATCC (Cat # 6710068, Manassas, VA). This clone contained the putative sequence for human NPQ. Random-prime labeling was performed using ³²P-dCTP and Klenow DNA polymerase was conducted as described in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1000258#s4" target="_blank">Materials and Methods</a>. 1. Brain, 2. Placenta, 3. Skeletal muscle, 4. Heart, 5. Kidney, 6. Pancreas, 7. Liver, 8. Lung, 9. Spleen, 10. Colon.</p