Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats

The aim of this study was to examine oxidative load and endothelium-dependent vasorelaxation in the serotonin pre-constricted femoral artery (FA) of Wistar-Kyoto (WKY) rats exposed to chronic social stress produced by crowding in the presence or absence of ascorbic acid (AsA) in working solution. Adult male rats were randomly divided into control (living space: 480 cm2/rat) or stressed (living space: 200 cm2/rat) groups for 8 weeks. Blood pressure and heart rate, determined using tail-cuff plethysmography, were not influenced by stress vs. control. Conjugated dienes (CD) and concentrations of thiobarbituric acid-reactive substances (TBARS) were measured in the left ventricle and liver (for assessment of oxidative load) and were found unchanged by chronic crowding. The nitric oxide (NO)-dependent component of endothelium-dependent relaxation was investigated in the FA using a wire myograph. In both the presence and absence of AsA, acetylcholine-induced relaxation of the FA of stressed rats significantly exceeded that of the controls, which was associated with an increase of the NO-dependent component. In conclusion, the data showed that chronic crowding did not produce oxidative stress in the organs investigated and indicate that elevation of NO production during chronic stress is an important way of adaptation, which may prevent normotensive rats from the development of stress-induced hypertension

First measurements with the CMS DAQ and timing hub prototype-1

The DAQ and Timing Hub is an ATCA hub board designed for the Phase-2 upgrade of the CMS experiment. In addition to providing high-speed Ethernet connectivity to all back-end boards, it forms the bridge between the sub-detector electronics and the central DAQ, timing, and trigger control systems. One important requirement is the distribution of several high-precision, phasestable, and LHC-synchronous clock signals for use by the timing detectors. The current paper presents first measurements performed on the initial prototype, with a focus on clock quality. It is demonstrated that the current design provides adequate clock quality to satisfy the requirements of the Phase-2 CMS timing detectors

Anaesthetic Impairment of Immune Function Is Mediated via GABAA Receptors

GABA(A) receptors are members of the Cys-loop family of neurotransmitter receptors, proteins which are responsible for fast synaptic transmission, and are the site of action of wide range of drugs. Recent work has shown that Cys-loop receptors are present on immune cells, but their physiological roles and the effects of drugs that modify their function in the innate immune system are currently unclear. We are interested in how and why anaesthetics increase infections in intensive care patients; a serious problem as more than 50% of patients with severe sepsis will die. As many anaesthetics act via GABA(A) receptors, the aim of this study was to determine if these receptors are present on immune cells, and could play a role in immunocompromising patients.We demonstrate, using RT-PCR, that monocytes express GABA(A) receptors constructed of α1, α4, β2, γ1 and/or δ subunits. Whole cell patch clamp electrophysiological studies show that GABA can activate these receptors, resulting in the opening of a chloride-selective channel; activation is inhibited by the GABA(A) receptor antagonists bicuculline and picrotoxin, but not enhanced by the positive modulator diazepam. The anaesthetic drugs propofol and thiopental, which can act via GABA(A) receptors, impaired monocyte function in classic immunological chemotaxis and phagocytosis assays, an effect reversed by bicuculline and picrotoxin.Our results show that functional GABA(A) receptors are present on monocytes with properties similar to CNS GABA(A) receptors. The functional data provide a possible explanation as to why chronic propofol and thiopental administration can increase the risk of infection in critically ill patients: their action on GABA(A) receptors inhibits normal monocyte behaviour. The data also suggest a potential solution: monocyte GABA(A) receptors are insensitive to diazepam, thus the use of benzodiazepines as an alternative anesthetising agent may be advantageous where infection is a life threatening problem

Estimation of Electromagnetic Compatibility Between DVB-T/DVB-T2 and 4G/5G in the 700 MHz Band for Co-Channel Case

Abstract The 694–790 MHz band (700 MHz) known also as the second digital dividend was allocated to the mobile radiocommunication service on a primary basis in Region 1 and identified to International Mobile Telecommunications by the World Radiocommunication Conference 2012 (WRC-12). The designation of mobile service in Europe and other countries of Region 1 in 700 MHz band was obtained after the World Radiocommunication Conference 2015 (WRC-15). Administrations of Region 1 will be able to plan and use these frequencies for mobile networks, including IMT. The goal of this study is to estimate the electromagnetic compatibility of Digital Video Broadcasting – Terrestrial (DVB-T/DVB-T2) and LTE (Long Term Evolution) technology operating both in 700 MHz band. The study assumes frequency division duplex (FDD) channel arrangement of 703–733 MHz (for uplink) and of 758–788 MHz (for downlink). The model contains two parts: a DVB-T/DVB-T2 system and LTE mobile broadband network. Co-channel scenario is considered in this paper, and possible impact of DVB-T/DVB-T2 on LTE base stations (receivers) is also investigated. The Monte Carlo simulations within SEAMCAT software and the Minimum Coupling Loss (MCL) method are used for interference investigation. The coordination trigger field strength value predetermined by GE06 Agreement is also used in this study. The Monte Carlo method presents more relaxed electromagnetic compatibility scenario in comparison with the MCL method. For SEAMCAT simulations, ITU-R P.1546-5 radio propagation model is used. The obtained results present the required minimum separation distance between DVB-T/DVB-T2 and LTE networks in the 694–790 MHz in order to provide the necessary performance of LTE mobile network.</jats:p