31 research outputs found
A ‘Dilute and Shoot’ Liquid Chromatography-Mass Spectrometry Method for Multiclass Drug Analysis in Pre-Cut Dried Blood Spots
Drugs able to affect the auditory and nervous systems and consumed by workers to
treatdifferent pathologies can represent a possible source of risk in the work environment. All the
target compounds involved in the presented project show ototoxic and/or narcoleptic side effects and,
for these reasons, occupational safety organizations have recognized them as potential causes of work
injuries. A multiclass method for the analysis of 15 drugs among the most widespread worldwide
(belonging to nine different classes including antihistamines, beta-blockers, antidepressants, Z-drugs
and opioids), was developed and validated. This study describes a rapid, sensitive and effective
method to analyse these substances in whole blood using tailored pre-cut dried blood spots. Detection
was achieved with a triple quadrupole mass spectrometer after an easy and simple ‘dilute and shoot’
solubilisation followed by an UPLC separation. All the issues linked to the use of the dried blood
spots and whole blood, such as haematocrit variability, volumetric evaluation and sample carrier
choice were carefully studied and managed during method development. From the validation study
results it emerged that this approach can be deemed successful thanks to its few pg L1 LOQs,
good linear intervals, absolute recoveries of no less than 75%, an almost negligible matrix effect and
accuracy and precision in line with the European and American guidelines for validation. All the
obtained goals have been specifically pursued in order to encourage method diffusion as a primary
prevention intervention, even in small private workplaces
Polymyxin-based photosensitizer for the potent and selective killing of Gram-negative bacteria
A Tetrazine-Labile Vinyl Ether Benzyloxycarbonyl Protecting Group (VeZ): An Orthogonal Tool for Solid-Phase Peptide Chemistry
The
vinyl ether benzyloxycarbonyl (VeZ) protecting group is selectively
cleaved by treatment with tetrazines via an inverse electron-demand
Diels–Alder reaction. This represents a new orthogonal protecting
group for solid-phase peptide synthesis, with Fmoc-LysÂ(VeZ)-OH as
a versatile alternative to Fmoc-LysÂ(Alloc)-OH and Fmoc-LysÂ(Dde)-OH,
as demonstrated by the synthesis of two biologically relevant cyclic
peptides