Contemporary analytical techniques reveal the secret composition of a 19th century Jerusalem Balsam

In 1719, Antonio Menzani di Cuna from the Saint Savior monastery published an alcoholic extract formula made from plant and herb resins under the name Jerusalem Balsam. The Balsam gained high popularity due to its remedial benefits. At the end of the 19 th century, Jerusalem Balsam produced by the hermit Johannes Treutler was found to be particularly popular. We analysed a sample of a valuable find coming from the last decade of the 19 th century, making it probably the oldest surviving Jerusalem Balsam in the world. The purpose of this work was to investigate the composition of the historical sample and to try to determine the origin of its components. This was achieved by comparing the profile of volatile compounds extracted from the balsam using HS-SPME technique with the profile characteristic for plant resins as classic ingredients of the Johannes Treutler formula. The use of two chromatographic columns of different polarity, as well as the transformation of the polar components of the sample into TMS derivatives, allowed to obtain new information on the historical composition of the Balsam. Also, it can be stated with high probability that plant resins were indeed used in the production of the Balsam as referred to in the original recipe of Johannes Treutler. We also discuss challenges in determining the original composition of the Balsam

Microbial diversity as an indicator of a diversified cropping system for luvisoils in a moderate climate. Case study – Long term experiments from Poland

The idea of agricultural sustainable in the EU is based on, both minimizing interference with the soil system as well as diversifying crop rotation what relates to the limited cultivation system (changed from plow to no-plowing tillage) as well as organic fertilization is often abandoned. Taking above into account, our goal was determined of the structure, composition, and metabolic profiles of soil microbiomes in various cultivation methods (under multiannual plow and no-plow cultivation) using metagenomic analysis. Having regard to the recommendations contained in EU report (European Commission et al., 2020) of the Mission board for Soil health and food, 2020 indicating the lack of microbiological indicators of “healthy soil”. So, we have tried to select of microbiological indicators showing sensitivity and resistance to use the methods of soil cultivation. The research object was located on almost 100-year field experiments at the Experimental Station of the Faculty of Agriculture and Biology in Skierniewice/near Warsaw on, luvisoils dominated in the temperate climate of Central Europe. Soil microorganisms respond with changes in their abundance and taxonomic composition depending on the methods of soil cultivation. Actinobacteria were the most abundant, while Planctomycetes were the least abundant in the metagenome of soil fertilized with manure, whereas the uncultivated soil was dominated by Nitrospirae. We can recommend the following taxa, including Gemmatimonas sp. as a microbiological indicator sensitive to the long-term lack of both plow cultivation of soil and organic fertilization, and Mycobacterium sp. as a resistancivity indicator to this soil cultivation method. Sorangium sp. could be recommended as microbiological indicators which responds by reducing the quantity under effect of the organically fertilized soil, while the plow and no-plow cultivation does not affect changes in its quantity. The use of various cultivation methods changed the biochemical functions in soil metagenoms, including nitrogen and sulfur metabolism and carbohydrate metabolism, and in the production of plant hormones and siderophores. Additionally, soil cultivation ways changed the response of microorganism’s stresses, including oxidative stress. The conducted research indicates the necessity to conduct further research on the influence of various cultivation methods, on the diversity of the microorganism community and soil metabolism. The result of which may be the selection of appropriate microbiological indicators for determining “soil health” depending on the type of soil under cultivation located in different climatic zones, not only presented in the paper